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[176084_at]

Description Y65B4A.F /REP_DB=WormBase Gene ID /WP=CE25532 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 76      4grp_total > 04      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 113
1 2 3 4 range
A 34
(-2.9)
A 		37.1
(-2.7)
A 		31.5
(-3)
A 		139.7
(3)
A 		108
B 60.2
(3)
A 		36.6
(-3)
A 		43
(-1.4)
A 		51.5
(0.8)
A 		24
C 57.3
(-1)
A 		26.9
(-3)
A 		30.2
(-2.8)
A 		119.9
(3)
A 		93
avr 50.5
(-1.5) 		33.5
(-3) 		34.9
(-2.9) 		103.7
(3) 		70

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 90
1 2 3 4 5 6 range
A 17.7
(-3)
A 		45
(-1.2)
A 		57.2
(-0.4)
A 		79.5
(1.1)
A 		61.6
(-0.1)
A 		107.9
(3)
P 		90
B 41.9
(-3)
A 		42.3
(-2.9)
A 		59.5
(-0.1)
A 		42.5
(-2.9)
A 		48.3
(-1.9)
P 		77.8
(3)
P 		36
C 60.1
(-0.6)
A 		71
(0.8)
A 		73.3
(1.1)
A 		41.8
(-3)
A 		60.3
(-0.6)
A 		87.4
(3)
P 		46
avr 39.9
(-3) 		52.8
(-1.5) 		63.3
(-0.3) 		54.6
(-1.3) 		56.7
(-1) 		91
(3) 		51
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 13
1 2 3 4 range
A 12.4
(-0.9)
A 		8.1
(-3)
A 		8.2
(-3)
A 		20.5
(3)
A 		12
B 19.2
(3)
A 		9
(-3)
A 		10
(-2.4)
A 		11
(-1.8)
A 		10
C 19
(2.4)
A 		7.7
(-3)
A 		10.5
(-1.7)
A 		20.2
(3)
A 		13
avr 16.9
(2.8) 		8.3
(-3) 		9.6
(-2.1) 		17.2
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 26
1 2 3 4 5 6 range
A 3.3
(-3)
A 		16
(0.3)
A 		12.5
(-0.6)
A 		20.2
(1.4)
A 		18.6
(1)
A 		26.3
(3)
P 		23
B 14.6
(-1.9)
A 		14.1
(-2.1)
A 		14.5
(-1.9)
A 		11.3
(-3)
A 		19.6
(-0.2)
P 		29
(3)
P 		18
C 22.3
(1)
A 		21.7
(0.8)
A 		18.7
(-0)
A 		8.4
(-3)
A 		19.5
(0.2)
A 		29.3
(3)
P 		21
avr 13.4
(-3) 		17.3
(-1.4) 		15.2
(-2.2) 		13.3
(-3) 		19.2
(-0.6) 		28.2
(3) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176084_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18871
Sequence Type Exemplar sequence
Representative Public ID Y65B4A.F NCBI
Target Description Y65B4A.F /REP_DB=WormBase Gene ID /WP=CE25532 /CHR=1 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058356(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058356 NCBI Caenorhabditis elegans F56A6.4 (F56A6.4) mRNA, complete cds. 11/11 None
F56A6.4 ENSEMBL cdna:known chromosome:CEL140:I:614726:629447:-1 gene:F56A6.4 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000032534 4/11 Cross Hyb Matching Probes None
GENEFINDER00000032550 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:623630-630345(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F56A6.4
Entrez Gene 171652 Entrez gene
SwissProt Q9GZG5 EMBL-EBI
WormBase CE34319 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF99975 Hypothetical protein F56A6.4 [Caenorhabditis elegans] ref|NP_490757.2| F56A6.4 [Caenorhabditis elegans] 0.0
blast T33081 hypothetical protein F56A6.4 - Caenorhabditis elegans 4.0E-81
blast CAE61312 Hypothetical protein CBG05147 [Caenorhabditis briggsae] 2.0E-36
Sequence
Target Sequence 
>C. ELEGANS:176084_AT
gctcatactcgttgtgctcaagctcacaagagaatactgacataaatgtcgtggcgagac
ccgaaaaacggaaactaaccgacgatgagaaggagacgcagaaagtccacgggtctcaca
gcgaaaatatttactctccaaatagcaaatttccccttaaaaatttaaaacttattcagg
aactccgagaaaatgcgaaacgagctcgggaagctgaaaaagagagaaaaacgctggaaa
aggataaaaaaaagagcgaaatggcgcggagaagagaggaaaaagagaaagacaaggaaa
ctcgaaaaatcgaacgcgaaatttcggcggcaaccaattcgaaatgcgagctctacacgt
tttgccacgtggggaagactgtaattgacacgtggcatggcctcgaagcggaactcagtg
tactgtacgtggagcgaaaaatcgataatcaactgaaaatcgatacatcactgggaaccc
gcatcgaatggcgacgaaaatgtgtcgaattgcgtgaagatgatagcgggagaatcaaca
caaaacttgttcgctgtcgtggttccggcggagacgttga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCTCATACTCGTTGTGCTCAAGCTC 473 301 440 Antisense
AGACGCAGAAAGTCCACGGGTCTCA 128 71 533 Antisense
GAAATTTCGGCGGCAACCAATTCGA 515 361 757 Antisense
CGAAATGCGAGCTCTACACGTTTTG 679 251 779 Antisense
CGTTTTGCCACGTGGGGAAGACTGT 204 245 797 Antisense
GTAATTGACACGTGGCATGGCCTCG 155 461 820 Antisense
TGGCCTCGAAGCGGAACTCAGTGTA 544 543 837 Antisense
AATCGATACATCACTGGGAACCCGC 111 169 897 Antisense
GGAACCCGCATCGAATGGCGACGAA 211 529 913 Antisense
CAAAACTTGTTCGCTGTCGTGGTTC 29 191 980 Antisense
GTCGTGGTTCCGGCGGAGACGTTGA 446 471 995 Antisense
Sequence Source Affymetrix Proprietary Database