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[176073_at]

Description ZK370.6 /REP_DB=WormBase Gene ID /WP=CE00398 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 20      6grp_high1_10_10 > 14      4grp_highest_8_8 > 39      4grp_high_8_8 > 12      6grp_highest_nonorm > 80      6grp_total > 03      4grp_total > 12      6grp_highest_sub > De      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 667
1 2 3 4 range
A 386.7
(0.3)
P 		168.6
(-2.6)
P 		136.8
(-3)
P 		592
(3)
P 		455
B 304.3
(-1.3)
P 		228.5
(-2.1)
P 		149.3
(-3)
P 		708.1
(3)
P 		559
C 378.7
(-1)
P 		203.2
(-2.6)
P 		160.6
(-3)
P 		803.8
(3)
P 		643
avr 356.6
(-0.7) 		200.1
(-2.4) 		148.9
(-3) 		701.3
(3) 		552

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 214
1 2 3 4 5 6 range
A 212.9
(0.7)
P 		283
(3)
P 		200.1
(0.2)
P 		239.2
(1.5)
P 		211.9
(0.6)
P 		103.4
(-3)
P 		180
B 317.1
(3)
P 		261.2
(1.4)
P 		191.1
(-0.6)
P 		157.7
(-1.6)
P 		200.9
(-0.4)
P 		109.5
(-3)
P 		208
C 275.1
(3)
P 		265.4
(2.5)
P 		198.4
(-1.2)
P 		250.4
(1.6)
P 		173.6
(-2.6)
P 		166
(-3)
P 		109
avr 268.4
(2.9) 		269.9
(3) 		196.5
(-0.1) 		215.8
(0.7) 		195.5
(-0.1) 		126.3
(-3) 		144
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 116
1 2 3 4 range
A 140.8
(3)
P 		36.6
(-3)
P 		35.8
(-3)
P 		86.7
(-0.1)
P 		105
B 97
(0.2)
P 		56.4
(-1.9)
P 		34.6
(-3)
P 		150.9
(3)
P 		116
C 125.4
(2.2)
P 		58.4
(-2.8)
P 		56
(-3)
P 		135.5
(3)
P 		80
avr 121.1
(2.8) 		50.5
(-2.4) 		42.1
(-3) 		124.4
(3) 		82

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 85
1 2 3 4 5 6 range
A 39.3
(-1.9)
P 		100.7
(3)
P 		43.6
(-1.5)
P 		60.7
(-0.2)
P 		63.9
(0.1)
P 		25.2
(-3)
P 		76
B 110.3
(3)
P 		86.9
(1)
P 		46.4
(-2.5)
P 		41.8
(-2.9)
P 		81.6
(0.5)
P 		40.8
(-3)
P 		70
C 102
(3)
P 		81
(0.6)
P 		50.5
(-3)
P 		50.4
(-3)
P 		56.2
(-2.3)
P 		55.6
(-2.4)
P 		52
avr 83.9
(2.3) 		89.5
(3) 		46.8
(-2.2) 		51
(-1.7) 		67.2
(0.3) 		40.5
(-3) 		49
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176073_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6976
Sequence Type Exemplar sequence
Representative Public ID ZK370.6 NCBI
Target Description ZK370.6 /REP_DB=WormBase Gene ID /WP=CE00398 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066528(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066528 NCBI Caenorhabditis elegans ZK370.6 (ZK370.6) mRNA, complete cds. 11/11 None
GENEFINDER00000004307 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8737945:8743742:-1 9/11 None
ZK370.6 ENSEMBL cdna:known chromosome:CEL140:III:8741638:8743742:-1 gene:ZK370.6 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000004297 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8742402-8743738(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK370.6
Entrez Gene 191302 Entrez gene
SwissProt Q02333 EMBL-EBI
WormBase CE00398 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA28207 Hypothetical protein ZK370.5 [Caenorhabditis elegans] ref|NP_498928.1| ZK370.5 [Caenorhabditis elegans] sp|Q02332|PDK_CAEEL Probable [pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial precursor (Pyruvate dehydrogenase kinase) 0.0
blast CAE62763 Hypothetical protein CBG06929 [Caenorhabditis briggsae] 0.0
blast O02623 [Pyruvate dehydrogenase [lipoamide]] kinase, mitochondrial precursor (Pyruvate dehydrogenase kinase) gb|AAB52573.1| pyruvate dehydrogenase kinase [Ascaris suum] 0.0
blast AAA28208 Hypothetical protein ZK370.6 [Caenorhabditis elegans] ref|NP_498929.1| ZK370.6 [Caenorhabditis elegans] sp|Q02333|YOL6_CAEEL Hypothetical protein ZK370.6 in chromosome III 2.0E-90
blast CAE62761 Hypothetical protein CBG06927 [Caenorhabditis briggsae] 2.0E-68
Protein Domains
Database ID Description E-Value
Pfam IPR003594 EMBL-EBI ATP-binding region, ATPase-like 8.3E-19
Sequence
Target Sequence 
>C. ELEGANS:176073_AT
gattctacttgcttttctaatgttcatacctggtgtggagacaaaagaaagtccagtgaa
atgtgaatattctgatgagaagaaagttagcgagtgtctacaggtattcttatatttcaa
gacgcgccaatgtcttacaatgaaaaaattacagccaatgcttgactacgctacaaaact
tcaagcggagaccggagctatgcagtttccacttcaaggtggccaagttttcaaccaact
ttgctctatctacaccgatttcaaggtcgaagcccaaaaagagtatatcagctgtaagat
agcagccactcaagcaatttctgaagcacaaggagcaaaaggatcatcaacagaagcgta
tttgactgaaatgtgtcgagcgatggatggatatttgagatgttctcatccaattatttt
ggcaaagtgtggttccgatgcatggacatta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATTCTACTTGCTTTTCTAATGTTC 673 431 15 Antisense
GTTAGCGAGTGTCTACAGGTATTCT 178 447 100 Antisense
GACGCGCCAATGTCTTACAATGAAA 450 381 135 Antisense
TACAGCCAATGCTTGACTACGCTAC 421 643 164 Antisense
GCGGAGACCGGAGCTATGCAGTTTC 126 293 199 Antisense
AGCTATGCAGTTTCCACTTCAAGGT 65 79 210 Antisense
GTTTTCAACCAACTTTGCTCTATCT 554 447 241 Antisense
TTTGCTCTATCTACACCGATTTCAA 152 669 254 Antisense
CACCGATTTCAAGGTCGAAGCCCAA 344 201 267 Antisense
GATAGCAGCCACTCAAGCAATTTCT 675 423 312 Antisense
GTGTGGTTCCGATGCATGGACATTA 698 487 441 Antisense
Sequence Source Affymetrix Proprietary Database