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[176060_at]

Description Y50D4A.I /REP_DB=WormBase Gene ID /WP=CE25348 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 55      4grp_total > 22      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 168.5
(2.9)
A 		171.6
(3)
A 		28.6
(-3)
A 		118.9
(0.8)
A 		143
B 192.4
(0.8)
M 		97.6
(-3)
A 		118.2
(-2.2)
A 		248.9
(3)
P 		151
C 179.5
(2.2)
A 		31.5
(-3)
A 		119
(0.1)
A 		202.5
(3)
M 		171
avr 180.1
(2.4) 		100.2
(-2.3) 		88.6
(-3) 		190.1
(3) 		102

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 118
1 2 3 4 5 6 range
A 151.8
(3)
A 		121.3
(0.4)
A 		83.3
(-2.8)
A 		128.2
(1)
A 		90.1
(-2.2)
A 		80.9
(-3)
A 		71
B 95.5
(-2.8)
A 		98.4
(-2.6)
A 		156.2
(2.2)
A 		104.2
(-2.1)
A 		93.1
(-3)
A 		166.6
(3)
A 		74
C 81.6
(-0)
A 		98.6
(1.5)
A 		105.1
(2.1)
A 		115.1
(3)
A 		48.2
(-3)
A 		108
(2.4)
M 		67
avr 109.6
(1.7) 		106.1
(1.2) 		114.9
(2.5) 		115.8
(2.6) 		77.1
(-3) 		118.5
(3) 		41
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 54
1 2 3 4 range
A 61.3
(3)
A 		37.3
(0.3)
A 		7.5
(-3)
A 		17.4
(-1.9)
A 		54
B 61.4
(3)
M 		24.1
(-3)
A 		27.4
(-2.5)
A 		53
(1.6)
P 		37
C 59.4
(3)
A 		9.1
(-3)
A 		41.5
(0.9)
A 		34.1
(-0)
M 		50
avr 60.7
(3) 		23.5
(-3) 		25.5
(-2.7) 		34.8
(-1.2) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 47
1 2 3 4 5 6 range
A 28
(-0.6)
A 		43.1
(3)
A 		18.1
(-3)
A 		32.5
(0.5)
A 		27.2
(-0.8)
A 		19.7
(-2.6)
A 		25
B 33.2
(-2)
A 		32.7
(-2.1)
A 		37.9
(-1.2)
A 		27.6
(-3)
A 		37.8
(-1.2)
A 		62.1
(3)
A 		35
C 30.2
(1.3)
A 		30.1
(1.2)
A 		26.8
(0.3)
A 		23.1
(-0.8)
A 		15.6
(-3)
A 		36.1
(3)
M 		21
avr 30.5
(-1.3) 		35.3
(1.1) 		27.6
(-2.7) 		27.7
(-2.6) 		26.9
(-3) 		39.3
(3) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176060_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19466
Sequence Type Exemplar sequence
Representative Public ID Y50D4A.I NCBI
Target Description Y50D4A.I /REP_DB=WormBase Gene ID /WP=CE25348 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070993(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070993 NCBI Caenorhabditis elegans Y50D4A.1 (Y50D4A.1) mRNA, complete cds. 10/11 A 
SNAP00000016433 ENSEMBL cdna:SNAP chromosome:CEL140:V:1207139:1219619:1 10/11 None
GENEFINDER00000016435 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:1210185:1219619:1 10/11 None
Y50D4A.1 ENSEMBL cdna:known chromosome:CEL140:V:1207719:1219676:1 gene:Y50D4A.1 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:1210184-1214952(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4A.1
Entrez Gene 178618 Entrez gene
SwissProt Q95Y57 EMBL-EBI
WormBase CE27637 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6412 protein biosynthesis inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5622 intracellular inferred from electronic annotation QuickGO AmiGO
5840 ribosome inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3735 structural constituent of ribosome inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68502 Hypothetical protein Y50D4A.1 [Caenorhabditis elegans] ref|NP_503394.1| Y50D4A.1 [Caenorhabditis elegans] 0.0
blast CAE66580 Hypothetical protein CBG11897 [Caenorhabditis briggsae] 1.0E-128
blast CAE66580 Hypothetical protein CBG11897 [Caenorhabditis briggsae] 1.0E-124
blast CAE58014 Hypothetical protein CBG01084 [Caenorhabditis briggsae] 1.0E-103
blast CAE58014 Hypothetical protein CBG01084 [Caenorhabditis briggsae] 2.0E-98
Sequence
Target Sequence 
>C. ELEGANS:176060_AT
aatcggacttcagcttcacatttccgagcaaaaatgtaaagatcttctgaagatagtcat
cgaaacgattcgaaccaagtacacagaaagcctagaagaagccggcctggatgaggatga
agcttggagcaaagctcttgctctagtggactctaggccaccaatcgaagagtcacaact
ctacaaggctttggaaattatttgtaaacacattgagaaggccgagaccactggaagctt
gctgagccacgaacaacttccaagcataattcgaaaaacggaaagtttcgattttttgaa
tttttttagttcgaaaaatagacgaaataaaaaagtgcgcgagggaagtgcaaactccgc
ccatttctccgcccctttccctcgactaactaccggcttttttgaacaccttcgccgaga
atttcctcttcaaatcaagctacactgccttctatggagctacaaacgattagatgtttg
ggagaaaatttgtgtggaagaaggaaaaactcggtacgaaactgtcctaaaatccgagat
cgccaacccgctggccagcaagatgacct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATCGGACTTCAGCTTCACATTTCC 153 169 387 Antisense
GCAAAGCTCTTGCTCTAGTGGACTC 178 317 515 Antisense
CTAGGCCACCAATCGAAGAGTCACA 234 213 539 Antisense
GAGACCACTGGAAGCTTGCTGAGCC 328 395 610 Antisense
GCTGAGCCACGAACAACTTCCAAGC 389 295 627 Antisense
CGCGAGGGAAGTGCAAACTCCGCCC 522 241 724 Antisense
TGAACACCTTCGCCGAGAATTTCCT 72 573 789 Antisense
GAATTTCCTCTTCAAATCAAGCTAC 290 325 805 Antisense
GCTACACTGCCTTCTATGGAGCTAC 334 301 825 Antisense
CCTAAAATCCGAGATCGCCAACCCG 646 195 912 Antisense
AACCCGCTGGCCAGCAAGATGACCT 96 147 931 Antisense
Sequence Source Affymetrix Proprietary Database