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[176046_at]

Description Y41D4A.C /REP_DB=WormBase Gene ID /WP=CE21848 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 57      6grp_high1_10_10 > 74      4grp_highest_8_8 > 01      4grp_high_8_8 > 45      6grp_highest_nonorm > 90      6grp_total > 64      4grp_total > 50      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 143
1 2 3 4 range
A 142.3
(-0.7)
P 		152.9
(-0.1)
P 		208
(3)
P 		101.4
(-3)
P 		107
B 152.6
(-3)
P 		244.3
(3)
P 		154.4
(-2.9)
P 		180.1
(-1.2)
P 		92
C 147.3
(-3)
P 		191.5
(3)
P 		176.4
(1)
P 		150.1
(-2.6)
P 		44
avr 147.4
(-2.6) 		196.2
(3) 		179.6
(1.1) 		143.9
(-3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 105
1 2 3 4 5 6 range
A 126.5
(-3)
P 		140.5
(2.6)
P 		140.8
(2.8)
P 		141.4
(3)
P 		133.9
(-0)
P 		141.3
(3)
P 		15
B 96
(-2.7)
P 		113.9
(0)
P 		108.5
(-0.8)
P 		119.6
(0.9)
P 		133.9
(3)
P 		93.9
(-3)
P 		40
C 153.7
(-0.1)
P 		111.3
(-3)
P 		183
(1.9)
P 		123.7
(-2.2)
P 		130.8
(-1.7)
P 		199.3
(3)
P 		88
avr 125.4
(-2.1) 		121.9
(-3) 		144.1
(2.8) 		128.2
(-1.3) 		132.9
(-0.1) 		144.8
(3) 		23
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 47
1 2 3 4 range
A 51.8
(2.6)
P 		33.2
(-0.2)
P 		54.4
(3)
P 		14.8
(-3)
P 		40
B 48.7
(0.2)
P 		60.3
(3)
P 		35.8
(-3)
P 		38.4
(-2.4)
P 		25
C 48.7
(0.9)
P 		55.1
(1.9)
P 		61.5
(3)
P 		25.3
(-3)
P 		36
avr 49.7
(2.8) 		49.5
(2.7) 		50.6
(3) 		26.2
(-3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 43
1 2 3 4 5 6 range
A 23.3
(-3)
P 		50
(3)
P 		30.7
(-1.3)
P 		35.9
(-0.2)
P 		40.4
(0.8)
P 		34.4
(-0.5)
P 		27
B 33.4
(-1.5)
P 		37.9
(-0.5)
P 		26.3
(-3)
P 		31.7
(-1.8)
P 		54.4
(3)
P 		35
(-1.1)
P 		28
C 57
(1.6)
P 		34
(-1.7)
P 		46.6
(0.1)
P 		24.9
(-3)
P 		42.3
(-0.5)
P 		66.7
(3)
P 		42
avr 37.9
(-0.1) 		40.6
(0.9) 		34.5
(-1.5) 		30.8
(-3) 		45.7
(3) 		45.4
(2.9) 		15
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176046_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19278
Sequence Type Exemplar sequence
Representative Public ID Y41D4A.C NCBI
Target Description Y41D4A.C /REP_DB=WormBase Gene ID /WP=CE21848 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067728(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067728 NCBI Caenorhabditis elegans Y41D4A.6 (Y41D4A.6) mRNA, complete cds. 11/11 A 
SNAP00000028254 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1728418:1759946:-1 11/11 None
GENEFINDER00000028263 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1741094:1745773:-1 11/11 None
Y41D4A.6 ENSEMBL cdna:known chromosome:CEL140:IV:1741094:1745517:-1 gene:Y41D4A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1743946-1746072(-) 97.97 100.0
Public Domain and Genome References
Gene Title
Ensembl Y41D4A.6
Entrez Gene 176982 Entrez gene
SwissProt Q95Y25 EMBL-EBI
WormBase CE27621 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256728_AT glutamyl-tRNA(Gln) amidotransferase, putative at
CANINE_2:CFA.18543.1.S1_S_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
CANINE_2:CFA.2234.1.S1_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
CANINE_2:CFAAFFX.6541.1.S1_S_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
DROSGENOME1:141724_AT dm
DROSOPHILA_2:1628959_AT dm
CHICKEN:GGA.11968.1.S1_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 gga
HG-U133_PLUS_2:233089_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133B:233089_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HU35KSUBB:RC_N51340_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133_PLUS_2:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A_2:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133_PLUS_2:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A_2:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:G8922807_3P_X_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:G12052881_3P_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:HS.210778.1.A1_3P_X_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:HS.210778.1.A1_3P_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:218949_3P_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95C:62794_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95E:78338_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95E:90930_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
MOE430A:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430A_2:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430_2:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MU11KSUBA:C80053_RC_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MG-U74AV2:161071_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOE430A:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430A_2:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430_2:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MU19KSUBC:TC38735_AT Glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1, mRNA (cDNA clone MGC:91336 IMAGE:30605375) mm
RG-U34B:RC_AA997173_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAE230B:1380842_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAT230_2:1380842_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAE230B:1383831_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAT230_2:1383831_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4040 amidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68456 Hypothetical protein Y41D4A.6 [Caenorhabditis elegans] ref|NP_500129.1| Y41D4A.6 [Caenorhabditis elegans] 0.0
blast CAE63839 Hypothetical protein CBG08395 [Caenorhabditis briggsae] 1.0E-166
blast CAE63839 Hypothetical protein CBG08395 [Caenorhabditis briggsae] 1.0E-165
blast AAK68455 Hypothetical protein Y41D4A.5 [Caenorhabditis elegans] ref|NP_500128.1| Y41D4A.5 [Caenorhabditis elegans] 1.0E-138
blast EAA05381 ENSANGP00000011128 [Anopheles gambiae str. PEST] ref|XP_309611.2| ENSANGP00000011128 [Anopheles gambiae str. PEST] 2.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 1.2E-114
Pfam IPR000242 EMBL-EBI Tyrosine specific protein phosphatase 1.1E-78
Pfam IPR000120 EMBL-EBI Amidase 1.4E-105
Pfam IPR000120 EMBL-EBI Amidase 1.2E-111
Sequence
Target Sequence 
>C. ELEGANS:176046_AT
ttctgtatgggaacttcgtcggctctcggacattttggaccggtgaagagtgatttgacg
gagcctggtggcactgaggattggactattccgggaggaagctctgggggctctgcagtt
gccgttcagactggaatcgctgatttcgctctcggctcggataccggcggttcaaccaga
aatcctgctgcttttaacggaattttcggtttcaagccaacttatggagttttatcacgc
aatggcttgattcctctggtgaattcactggatgctccgtcgattttcgcgaaaaccgct
caaaaatgttgggaattgctggaattatcgaagggcattgataaaaacgactcaacaagt
gtagaacttccagaaaaagctggaattagctctctggaagggctgaaaatcggaattcct
gtggaatatcacaacgaatgcctgacagaagacgcgtggaggcactggaatcgggttaca
gggcttttgaagagccggggagctgctgaaattcatgtgctgaaccttccgacaactaga
ttctcgctggcttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTCTGTATGGGAACTTCGTCGGCTC 18 693 493 Antisense
GTCGGCTCTCGGACATTTTGGACCG 603 471 510 Antisense
GACGGAGCCTGGTGGCACTGAGGAT 399 377 549 Antisense
GTTGCCGTTCAGACTGGAATCGCTG 442 439 610 Antisense
CTCGGATACCGGCGGTTCAACCAGA 694 209 648 Antisense
CCAGAAATCCTGCTGCTTTTAACGG 560 271 668 Antisense
ATCACGCAATGGCTTGATTCCTCTG 133 29 726 Antisense
TGGTGAATTCACTGGATGCTCCGTC 583 551 749 Antisense
ATGCTCCGTCGATTTTCGCGAAAAC 181 41 764 Antisense
ATTCATGTGCTGAACCTTCCGACAA 119 11 1003 Antisense
CGACAACTAGATTCTCGCTGGCTTG 641 251 1022 Antisense
Sequence Source Affymetrix Proprietary Database