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[176019_at]

Description Y41D4A.D /REP_DB=WormBase Gene ID /WP=CE21835 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 4grp_high_8_8 > 38      6grp_total > 54      4grp_total > 33      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 174
1 2 3 4 range
A 250.4
(-3)
P 		261.5
(-2.1)
P 		283.8
(-0.2)
P 		321.8
(3)
P 		71
B 157.9
(-3)
P 		248.1
(3)
P 		246
(2.9)
P 		232.4
(2)
P 		90
C 221
(-3)
M 		331.7
(3)
P 		257.9
(-1)
P 		241.5
(-1.9)
P 		111
avr 209.8
(-3) 		280.4
(3) 		262.6
(1.5) 		265.2
(1.7) 		71

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 209
1 2 3 4 5 6 range
A 160.8
(-2.5)
P 		304.2
(3)
P 		177.5
(-1.9)
P 		147.5
(-3)
A 		214.4
(-0.4)
A 		233.8
(0.3)
M 		157
B 150.7
(-0.1)
P 		98.7
(-2.8)
M 		172.8
(1)
P 		95
(-3)
A 		152.2
(-0.1)
M 		211.8
(3)
P 		117
C 152.8
(-1.5)
P 		118.6
(-3)
A 		254.2
(3)
P 		172.5
(-0.6)
P 		129.7
(-2.5)
M 		121.6
(-2.9)
P 		136
avr 154.8
(-1.4) 		173.8
(0.4) 		201.5
(3) 		138.3
(-3) 		165.4
(-0.4) 		189.1
(1.8) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 55
1 2 3 4 range
A 91.2
(3)
P 		56.8
(-1.7)
P 		74.2
(0.7)
P 		47.1
(-3)
P 		44
B 50.3
(-2.6)
P 		61.3
(3)
P 		57
(0.8)
P 		49.5
(-3)
P 		12
C 73.2
(0.6)
M 		95.4
(3)
P 		89.9
(2.4)
P 		40.7
(-3)
P 		55
avr 71.6
(2.5) 		71.2
(2.5) 		73.7
(3) 		45.8
(-3) 		28

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 83
1 2 3 4 5 6 range
A 29.6
(-3)
P 		108.2
(3)
P 		38.6
(-2.3)
P 		37.4
(-2.4)
A 		64.7
(-0.3)
A 		56.9
(-0.9)
M 		79
B 52.4
(0)
P 		32.8
(-2.2)
M 		42
(-1.1)
P 		25.2
(-3)
A 		61.8
(1.1)
M 		79
(3)
P 		54
C 56.6
(1.4)
P 		36.2
(-2.7)
A 		64.7
(3)
P 		34.7
(-3)
P 		42
(-1.5)
M 		40.7
(-1.8)
P 		30
avr 46.2
(0.1) 		59.1
(3) 		48.4
(0.6) 		32.4
(-3) 		56.2
(2.3) 		58.9
(3) 		27
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176019_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19171
Sequence Type Exemplar sequence
Representative Public ID Y41D4A.D NCBI
Target Description Y41D4A.D /REP_DB=WormBase Gene ID /WP=CE21835 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067728(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067728 NCBI Caenorhabditis elegans Y41D4A.6 (Y41D4A.6) mRNA, complete cds. 11/11 A 
SNAP00000028254 ENSEMBL cdna:SNAP chromosome:CEL140:IV:1728418:1759946:-1 11/11 None
GENEFINDER00000028263 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:1741094:1745773:-1 11/11 None
Y41D4A.6 ENSEMBL cdna:known chromosome:CEL140:IV:1741094:1745517:-1 gene:Y41D4A.6 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:1741099-1741572(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y41D4A.6
Entrez Gene 176982 Entrez gene
SwissProt Q95Y25 EMBL-EBI
WormBase CE27621 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:256728_AT glutamyl-tRNA(Gln) amidotransferase, putative at
CANINE_2:CFA.18543.1.S1_S_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
CANINE_2:CFA.2234.1.S1_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
CANINE_2:CFAAFFX.6541.1.S1_S_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 cfa
DROSGENOME1:141724_AT dm
DROSOPHILA_2:1628959_AT dm
CHICKEN:GGA.11968.1.S1_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 gga
HG-U133_PLUS_2:233089_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133B:233089_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HU35KSUBB:RC_N51340_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133_PLUS_2:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A_2:218948_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133_PLUS_2:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U133A_2:218949_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:G8922807_3P_X_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:G12052881_3P_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:HS.210778.1.A1_3P_X_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:HS.210778.1.A1_3P_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
U133_X3P:218949_3P_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95C:62794_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95E:78338_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
HG-U95E:90930_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 hs
MOE430A:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430A_2:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430_2:1456542_S_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MU11KSUBA:C80053_RC_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MG-U74AV2:161071_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOE430A:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430A_2:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MOUSE430_2:1429317_AT glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 mm
MU19KSUBC:TC38735_AT Glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1, mRNA (cDNA clone MGC:91336 IMAGE:30605375) mm
RG-U34B:RC_AA997173_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAE230B:1380842_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAT230_2:1380842_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAE230B:1383831_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
RAT230_2:1383831_AT similar to glutaminyl-tRNA synthase (glutamine-hydrolyzing)-like 1 rn
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4040 amidase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68456 Hypothetical protein Y41D4A.6 [Caenorhabditis elegans] ref|NP_500129.1| Y41D4A.6 [Caenorhabditis elegans] 0.0
blast CAE63839 Hypothetical protein CBG08395 [Caenorhabditis briggsae] 1.0E-166
blast CAE63839 Hypothetical protein CBG08395 [Caenorhabditis briggsae] 1.0E-165
blast AAK68455 Hypothetical protein Y41D4A.5 [Caenorhabditis elegans] ref|NP_500128.1| Y41D4A.5 [Caenorhabditis elegans] 1.0E-138
blast EAA05381 ENSANGP00000011128 [Anopheles gambiae str. PEST] ref|XP_309611.2| ENSANGP00000011128 [Anopheles gambiae str. PEST] 2.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000120 EMBL-EBI Amidase 1.2E-114
Pfam IPR000242 EMBL-EBI Tyrosine specific protein phosphatase 1.1E-78
Pfam IPR000120 EMBL-EBI Amidase 1.4E-105
Pfam IPR000120 EMBL-EBI Amidase 1.2E-111
Sequence
Target Sequence 
>C. ELEGANS:176019_AT
tcttcccggtcagaagccctgaatacaattgttcgacggcggattttcgctggaaattat
ttcctgatgaagcaatatcgccaaaagtactttgaaagggcgctaaaagtgcggagaatc
atccacgacgagttgcgccaggctttccgggaagtcgatttgattgtcacgccgacagcg
tctggaacagcgccgaaatattctgaattacgggatacgttgttctcgaaggaggataat
gatgattatttcactcaggccgccaatttggccgggatcccatcgatttctgttccagtt
ggagttgcggaagatggactgccgattggtgttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCTTCCCGGTCAGAAGCCCTGAATA 508 615 22 Antisense
GTTCGACGGCGGATTTTCGCTGGAA 282 439 52 Antisense
AGAATCATCCACGACGAGTTGCGCC 707 75 136 Antisense
CGCCAGGCTTTCCGGGAAGTCGATT 235 255 157 Antisense
GAAGTCGATTTGATTGTCACGCCGA 101 337 172 Antisense
CGACAGCGTCTGGAACAGCGCCGAA 28 253 194 Antisense
TACGGGATACGTTGTTCTCGAAGGA 178 645 230 Antisense
TGATGATTATTTCACTCAGGCCGCC 250 567 261 Antisense
TCAGGCCGCCAATTTGGCCGGGATC 182 619 276 Antisense
TCCCATCGATTTCTGTTCCAGTTGG 7 599 299 Antisense
GAAGATGGACTGCCGATTGGTGTTC 81 339 331 Antisense
Sequence Source Affymetrix Proprietary Database