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[176010_at]

Description K12H4.3 /REP_DB=WormBase Gene ID /WP=CE00268 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 76      6grp_high1_10_10 > 87      4grp_highest_8_8 > 26      4grp_high_8_8 > 58      6grp_highest_nonorm > 78      6grp_total > 76      4grp_total > 61      6grp_highest_sub > Da      6grp_high1_sub > In      6grp_total_sub > Increase      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1164
1 2 3 4 range
A 2265.9
(2.2)
P
2066.5
(0.3)
P
2356.1
(3)
P
1717.3
(-3)
P
639
B 2649.9
(3)
P
2030.2
(-1)
P
2248.1
(0.4)
P
1725.5
(-3)
P
924
C 2258.2
(1.4)
P
2124.6
(0.7)
P
2534.5
(3)
P
1485.9
(-3)
P
1049
avr 2391.3
(3)
2073.8
(0.5)
2379.6
(2.9)
1642.9
(-3)
748

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1238
1 2 3 4 5 6 range
A 1487.1
(-1.4)
P
1588.9
(-0.8)
P
1231.3
(-3)
P
1940.9
(1.3)
P
1478.1
(-1.5)
P
2211
(3)
P
980
B 1293.2
(-0.8)
P
1481.4
(0.6)
P
973
(-3)
P
1518.7
(0.8)
P
1469.7
(0.5)
P
1830.5
(3)
P
858
C 1526.5
(-1.9)
P
1412.9
(-3)
P
1880.6
(1.5)
P
1676.6
(-0.5)
P
1536.9
(-1.8)
P
2039.8
(3)
P
627
avr 1435.6
(-2.3)
1494.4
(-1.8)
1361.6
(-3)
1712.1
(0.2)
1494.9
(-1.8)
2027.1
(3)
666
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 633
1 2 3 4 range
A 825
(3)
P
448.6
(-0.9)
P
615.9
(0.8)
P
251.4
(-3)
P
574
B 845
(3)
P
501.4
(-1.3)
P
520.9
(-1.1)
P
367.7
(-3)
P
477
C 747.5
(1.7)
P
610.8
(0.4)
P
883.4
(3)
P
250.6
(-3)
P
633
avr 805.8
(3)
520.3
(-0.3)
673.4
(1.5)
289.9
(-3)
516

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 447
1 2 3 4 5 6 range
A 274.1
(-2.9)
P
565.1
(3)
P
268.2
(-3)
P
492.3
(1.5)
P
446
(0.6)
P
538.3
(2.5)
P
297
B 450
(-0.1)
P
493.1
(0.5)
P
236.3
(-3)
P
402.3
(-0.8)
P
597.1
(1.9)
P
682.6
(3)
P
446
C 565.8
(1)
P
431.1
(-1.4)
P
478.6
(-0.5)
P
337.2
(-3)
P
497.3
(-0.2)
P
682.8
(3)
P
346
avr 430
(-1)
496.4
(0.3)
327.7
(-3)
410.6
(-1.4)
513.5
(0.6)
634.6
(3)
307
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176010_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7825
Sequence Type Exemplar sequence
Representative Public ID K12H4.3 NCBI
Target Description K12H4.3 /REP_DB=WormBase Gene ID /WP=CE00268 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066355(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_066355 NCBI Caenorhabditis elegans K12H4.3 (K12H4.3) mRNA, complete cds. 11/11 None
SNAP00000000453 ENSEMBL cdna:SNAP chromosome:CEL140:III:8045967:8047418:1 11/11 None
GENEFINDER00000000471 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8045967:8047418:1 11/11 None
K12H4.3 ENSEMBL cdna:known chromosome:CEL140:III:8045956:8047525:1 gene:K12H4.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8045964-8047416(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K12H4.3
Entrez Gene 176134 Entrez gene
SwissProt P34524 EMBL-EBI
WormBase CE00268 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:260157_AT brix domain-containing protein at
DROSOPHILA_2:1624779_AT dm
DROSGENOME1:154073_AT dm
CHICKEN:GGAAFFX.11728.1.S1_S_AT brix domain containing 2 gga
HU35KSUBA:RC_AA454607_AT brix domain containing 2 hs
HG-U133A:219177_AT brix domain containing 2 hs
HG-U133_PLUS_2:219177_AT brix domain containing 2 hs
HG-U133A_2:219177_AT brix domain containing 2 hs
U133_X3P:G8922864_3P_AT brix domain containing 2 hs
HG-U95B:51968_AT brix domain containing 2 hs
MU11KSUBA:AA275260_S_AT brix domain containing 2 mm
MG-U74AV2:161746_I_AT brix domain containing 2 mm
MG-U74AV2:160266_R_AT brix domain containing 2 mm
MG-U74CV2:165682_R_AT brix domain containing 2 mm
MOE430A:1451144_AT brix domain containing 2 mm
MOUSE430A_2:1451144_AT brix domain containing 2 mm
MOUSE430_2:1451144_AT brix domain containing 2 mm
MOE430A:1423841_AT brix domain containing 2 mm
MOUSE430A_2:1423841_AT brix domain containing 2 mm
MOUSE430_2:1423841_AT brix domain containing 2 mm
MOE430B:1446733_AT Brix domain containing 2 (Bxdc2), mRNA mm
MOUSE430_2:1446733_AT Brix domain containing 2 (Bxdc2), mRNA mm
RG-U34C:RC_AI233407_AT similar to BRIX (predicted) rn
RAE230A:1389569_AT similar to BRIX (predicted) rn
RAT230_2:1389569_AT similar to BRIX (predicted) rn
RAE230B:1395479_AT similar to BRIX (predicted) rn
RAT230_2:1395479_AT similar to BRIX (predicted) rn
YEAST_2:1771396_AT Nucleolar protein, constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif Sc
Protein Similarities
Method ID Description E-Value
blast NP_498756 K12H4.3 [Caenorhabditis elegans] gb|AAA28097.1| Hypothetical protein K12H4.3 [Caenorhabditis elegans] sp|P34524|YM63_CAEEL Hypothetical protein K12H4.3 in chromosome III 0.0
blast CAE75065 Hypothetical protein CBG22980 [Caenorhabditis briggsae] 1.0E-166
blast AAH73014 LOC398268 protein [Xenopus laevis] 3.0E-75
Protein Domains
Database ID Description E-Value
Pfam IPR007109 EMBL-EBI Brix domain 4.7E-73
Sequence
Target Sequence
>C. ELEGANS:176010_AT
gaccagtcctttcatttgacgatgcttttgataagaaaccacaattgaagcttatcaagg
ccgtgcttatgcagactcttggaacaccacatcatcatccacgcagtcagccatttgtgg
atcacgtctttaatttttctgttggagaaggtgataaaatctggttccgcaattttcaaa
ttgttgatgagagtcttcaacttcaggaagttggaccacgcttcgttttggaaatggttc
gtttgttcgctggctcattcgaaggagccgtactgtacgacaacccaaactacgtctctc
caaatgtgatccgccgtgaacaccgtaaaggacaacattcgtatatcgagaaacagttgg
ctgtgaaagcatcaaatatcaaacaagctaaagtcacagaaattctcgctgaaaagactg
ttgatttggttggaaaggaattcgatacacaaaacaatgccgctgctgactcagaggctg
ctgctcagatc


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GACCAGTCCTTTCATTTGACGATGC 34 383 509 Antisense
AAGGCCGTGCTTATGCAGACTCTTG 603 161 565 Antisense
GACTCTTGGAACACCACATCATCAT 579 373 582 Antisense
GGATCACGTCTTTAATTTTTCTGTT 484 511 627 Antisense
TTGGACCACGCTTCGTTTTGGAAAT 436 707 719 Antisense
GGAAATGGTTCGTTTGTTCGCTGGC 516 527 738 Antisense
GTACGACAACCCAAACTACGTCTCT 669 455 783 Antisense
AACTACGTCTCTCCAAATGTGATCC 50 141 796 Antisense
TGATCCGCCGTGAACACCGTAAAGG 584 565 815 Antisense
ACAAAACAATGCCGCTGCTGACTCA 209 111 957 Antisense
TGACTCAGAGGCTGCTGCTCAGATC 367 571 975 Antisense
Sequence Source Affymetrix Proprietary Database