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[176003_at]

Description M02H5.1 /REP_DB=WormBase Gene ID /WP=CE25947 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 03      6grp_total > 26      4grp_total > 22      6grp_cgc > 04      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 83
1 2 3 4 range
A 100.7
(3)
P 		66.3
(-1.2)
A 		51.9
(-3)
A 		94.5
(2.2)
A 		49
B 116
(3)
P 		73.7
(-3)
P 		86.1
(-1.2)
A 		99.4
(0.6)
M 		42
C 110.3
(0.4)
P 		77.7
(-3)
A 		86.1
(-2.1)
P 		134.4
(3)
P 		57
avr 109
(2.9) 		72.6
(-3) 		74.7
(-2.7) 		109.4
(3) 		37

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 97
1 2 3 4 5 6 range
A 84.1
(2.3)
A 		91.6
(3)
A 		38.2
(-1.7)
A 		23.5
(-3)
A 		77.2
(1.7)
P 		23.3
(-3)
A 		68
B 103.1
(3)
P 		100.6
(2.8)
P 		21.6
(-3)
A 		21.7
(-3)
A 		63.1
(0.1)
P 		60.6
(-0.1)
P 		82
C 118.1
(3)
P 		86.9
(0.9)
P 		62.1
(-0.7)
A 		27.7
(-3)
A 		41.2
(-2.1)
A 		64.9
(-0.5)
P 		90
avr 101.8
(3) 		93
(2.3) 		40.6
(-1.7) 		24.3
(-3) 		60.5
(-0.2) 		49.6
(-1) 		78
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 36.7
(3)
P 		14.4
(-2.8)
A 		13.6
(-3)
A 		13.8
(-2.9)
A 		23
B 37
(3)
P 		18.2
(-3)
P 		20
(-2.4)
A 		21.2
(-2)
M 		19
C 36.5
(3)
P 		22.3
(-3)
A 		30
(0.3)
P 		22.7
(-2.8)
P 		14
avr 36.7
(3) 		18.3
(-3) 		21.2
(-2.1) 		19.2
(-2.7) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 39
1 2 3 4 5 6 range
A 15.5
(-0.8)
A 		32.6
(3)
A 		8.3
(-2.4)
A 		6
(-2.9)
A 		23.3
(0.9)
P 		5.7
(-3)
A 		27
B 35.9
(3)
P 		33.5
(2.5)
P 		5.2
(-3)
A 		5.7
(-2.9)
A 		25.6
(1)
P 		22.6
(0.4)
P 		31
C 43.8
(3)
P 		26.5
(0.3)
P 		15.8
(-1.4)
A 		5.6
(-3)
A 		13.3
(-1.8)
A 		21.7
(-0.5)
P 		38
avr 31.7
(3) 		30.9
(2.8) 		9.8
(-2.1) 		5.8
(-3) 		20.7
(0.5) 		16.7
(-0.5) 		26
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 176003_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15947
Sequence Type Exemplar sequence
Representative Public ID M02H5.1 NCBI
Target Description M02H5.1 /REP_DB=WormBase Gene ID /WP=CE25947 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070813(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070813 NCBI Caenorhabditis elegans Nuclear Hormone Receptor family member (nhr-99) (nhr-99) mRNA, complete cds. 11/11 None
M02H5.1 ENSEMBL cdna:known chromosome:CEL140:V:474638:476932:1 gene:M02H5.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
AF332213 9/11 Negative Strand Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:474637-476932(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol nhr-99
Ensembl M02H5.1
Entrez Gene 178569 Entrez gene
SwissProt Q966H4 EMBL-EBI
WormBase CE25947 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5634 nucleus inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3700 transcription factor activity inferred from electronic annotation QuickGO AmiGO
3707 steroid hormone receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast NP_503214 Nuclear Hormone Receptor family member (nhr-99) [Caenorhabditis elegans] gb|AAK84548.1| Nuclear hormone receptor family protein 99 [Caenorhabditis elegans] 0.0
blast AAK17984 nuclear receptor NHR-99 [Caenorhabditis elegans] 1.0E-112
blast AAG24179 Nuclear hormone receptor family protein 55 [Caenorhabditis elegans] ref|NP_503215.2| Nuclear Hormone Receptor family member (nhr-55) [Caenorhabditis elegans] gb|AAO39175.1| nuclear receptor NHR-55 [Caenorhabditis elegans] sp|O16962|NHR55_CAEEL Nuclear hormone receptor family member nhr-55 1.0E-111
Protein Domains
Database ID Description E-Value
Pfam IPR001628 EMBL-EBI Zn-finger, C4-type steroid receptor 3.5E-11
Sequence
Target Sequence 
>C. ELEGANS:176003_AT
tttagcgagtttcagcagctgcctgacgggttacagatgaaatttctcaaatcaatttgg
aaattgtggagcagatttgaaagacttgtcaccacaatgatagggaggagaaatagcgca
tgcagtagtaacatggtcctgataaagttggatgacacccaagtagtttttgatattaaa
aagatcgatgtagacgtgtcgtggcagtcgagatataatgtggagcaattgaaactattt
ggctacagttacctagacgaaaagtacgatgaaattatccaagaaatgatagatctggat
cccacaaatgtggagctctccttcatggtctgccaactgtgcctccaccacattggtaaa
aaattccaaggggatattctaaaagttgctgaacggctacaaagctctatagccaataat
ctacacgactactacgtgaataaagagaaaaggcccagatattcggggaggatcgccaaa
atgatgaagatcaataattatattcagaagggaatatacgagcggcgagtgaaagcagat
ttgatgagggtgtttgatgttttccacgtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTAGCGAGTTTCAGCAGCTGCCTG 253 673 664 Antisense
GCAGCTGCCTGACGGGTTACAGATG 356 311 678 Antisense
GGATGACACCCAAGTAGTTTTTGAT 521 511 813 Antisense
GTAGACGTGTCGTGGCAGTCGAGAT 678 455 853 Antisense
ATTTGGCTACAGTTACCTAGACGAA 527 15 900 Antisense
AATGTGGAGCTCTCCTTCATGGTCT 367 163 970 Antisense
CTGTGCCTCCACCACATTGGTAAAA 489 239 1000 Antisense
GTTGCTGAACGGCTACAAAGCTCTA 295 437 1048 Antisense
GCCAATAATCTACACGACTACTACG 26 275 1075 Antisense
GGCCCAGATATTCGGGGAGGATCGC 689 541 1115 Antisense
GAGGGTGTTTGATGTTTTCCACGTG 75 403 1209 Antisense
Sequence Source Affymetrix Proprietary Database