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[175999_at]

Description K12H4.5 /REP_DB=WormBase Gene ID /WP=CE00270 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 24      6grp_high1_10_10 > 32      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 15      6grp_total > 31      4grp_total > 59      6grp_high1_sub > 02_03      6grp_total_sub > Hat      6grp_rule_based > S3      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3348
1 2 3 4 range
A 4483.4
(0.5)
P 		5690.2
(3)
P 		5303.9
(2.2)
P 		2799.9
(-3)
P 		2890
B 4200.4
(0.6)
P 		5110.8
(3)
P 		5099.9
(3)
P 		2872.7
(-3)
P 		2238
C 4203.4
(-0.4)
P 		5542.8
(2)
P 		6075
(3)
P 		2727.1
(-3)
P 		3348
avr 4295.7
(0.3) 		5447.9
(2.9) 		5492.9
(3) 		2799.9
(-3) 		2693

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3245
1 2 3 4 5 6 range
A 4462.5
(-1)
P 		4901
(-0.1)
P 		6386.2
(3)
P 		5556.2
(1.3)
P 		4016.2
(-2)
P 		3534.9
(-3)
P 		2851
B 5549.7
(1.1)
P 		6058.1
(2.3)
P 		6337.7
(3)
P 		4874.6
(-0.4)
P 		4946.5
(-0.3)
P 		3785.9
(-3)
P 		2552
C 5064.4
(0.9)
P 		6075.6
(3)
P 		5994.8
(2.8)
P 		4888
(0.6)
P 		4482.6
(-0.3)
P 		3141.7
(-3)
P 		2934
avr 5025.5
(0.4) 		5678.2
(1.8) 		6239.6
(3) 		5106.3
(0.5) 		4481.8
(-0.8) 		3487.5
(-3) 		2752
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1707
1 2 3 4 range
A 1632.4
(3)
P 		1235.2
(1.1)
P 		1386.5
(1.8)
P 		409.9
(-3)
P 		1223
B 1339.5
(3)
P 		1262.2
(2.4)
P 		1181.8
(1.7)
P 		612.2
(-3)
P 		727
C 1391.4
(0.4)
P 		1593.6
(1.1)
P 		2117.3
(3)
P 		459.8
(-3)
P 		1658
avr 1454.4
(2.4) 		1363.7
(1.9) 		1561.9
(3) 		494
(-3) 		1068

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1194
1 2 3 4 5 6 range
A 822.6
(-3)
P 		1743
(3)
P 		1390.8
(0.7)
P 		1409.2
(0.8)
P 		1211.9
(-0.5)
P 		860.6
(-2.8)
P 		920
B 1931
(2.3)
P 		2016.5
(3)
P 		1539
(-0.9)
P 		1291.1
(-3)
P 		2009.7
(2.9)
P 		1411.7
(-2)
P 		725
C 1877.1
(3)
P 		1853.8
(2.8)
P 		1525.8
(0.6)
P 		983
(-3)
P 		1450.5
(0.1)
P 		1051.7
(-2.5)
P 		894
avr 1543.6
(0.4) 		1871.1
(3) 		1485.2
(-0) 		1227.8
(-2.1) 		1557.4
(0.5) 		1108
(-3) 		763
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175999_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7631
Sequence Type Exemplar sequence
Representative Public ID K12H4.5 NCBI
Target Description K12H4.5 /REP_DB=WormBase Gene ID /WP=CE00270 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066353(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066353 NCBI Caenorhabditis elegans K12H4.5 (K12H4.5) mRNA, complete cds. 11/11 None
GENEFINDER00000000475 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8042396:8044702:1 11/11 None
SNAP00000000451 ENSEMBL cdna:SNAP chromosome:CEL140:III:8044409:8044702:1 11/11 None
K12H4.5.2 ENSEMBL cdna:known chromosome:CEL140:III:8044409:8045125:1 gene:K12H4.5 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8044406-8044700(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl K12H4.5
Entrez Gene 187337 Entrez gene
SwissProt P34526 EMBL-EBI
WormBase CE00270 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_498754 K12H4.5 [Caenorhabditis elegans] gb|AAA28095.1| Hypothetical protein K12H4.5 [Caenorhabditis elegans] sp|P34526|YM65_CAEEL Hypothetical protein K12H4.5 in chromosome III 4.0E-53
blast NP_498754 K12H4.5 [Caenorhabditis elegans] gb|AAA28095.1| Hypothetical protein K12H4.5 [Caenorhabditis elegans] sp|P34526|YM65_CAEEL Hypothetical protein K12H4.5 in chromosome III 8.0E-53
blast CAE75063 Hypothetical protein CBG22978 [Caenorhabditis briggsae] 8.0E-50
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498754.1 1 30-47
K12H4.5.2 1 30-47
SNAP00000000451 1 30-47
Sequence
Target Sequence 
>C. ELEGANS:175999_AT
gatcaagtaccacgtgtcgcgaagcgcccgactctgggcttactggagcaccctgccaca
tcgaatgctcaagaaatacccggagcaaactatcttctacgcgactttcggagtagccac
actattgattggcggatataaaatgaagaagtacttgacagaaagtgataagccattcta
ccgcggatactacgacgtcgtccgttccaacgacccaatcgcacaaaattggagaaaacc
agaagaatatccagcaccgta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GATCAAGTACCACGTGTCGCGAAGC 84 423 15 Antisense
GCCCGACTCTGGGCTTACTGGAGCA 263 285 40 Antisense
ATCGAATGCTCAAGAAATACCCGGA 305 35 74 Antisense
TACCCGGAGCAAACTATCTTCTACG 133 649 91 Antisense
CTATCTTCTACGCGACTTTCGGAGT 691 223 104 Antisense
GACTTTCGGAGTAGCCACACTATTG 235 373 117 Antisense
GTAGCCACACTATTGATTGGCGGAT 180 457 127 Antisense
AGTGATAAGCCATTCTACCGCGGAT 561 57 178 Antisense
TTCTACCGCGGATACTACGACGTCG 82 667 190 Antisense
TTCCAACGACCCAATCGCACAAAAT 166 699 219 Antisense
AACCAGAAGAATATCCAGCACCGTA 391 147 251 Antisense
Sequence Source Affymetrix Proprietary Database