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[175991_s_at]

Description ZK686.3 /REP_DB=WormBase Gene ID /WP=CE00457 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 05      6grp_high1_10_10 > 20      4grp_highest_8_8 > 17      4grp_high_8_8 > 61      6grp_highest_nonorm > 46      6grp_total > 50      4grp_total > 59      6grp_highest_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 3794
1 2 3 4 range
A 3435.8
(0.5)
P 		4700.1
(2.5)
P 		5037.2
(3)
P 		1243.7
(-3)
P 		3794
B 3105.6
(0.1)
P 		3862.5
(1.8)
P 		4392.7
(3)
P 		1728.3
(-3)
P 		2664
C 3145.5
(0.2)
P 		3758
(1.3)
P 		4729.8
(3)
P 		1287
(-3)
P 		3443
avr 3229
(0.3) 		4106.9
(1.9) 		4719.9
(3) 		1419.7
(-3) 		3300

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1383
1 2 3 4 5 6 range
A 3163
(-0.4)
P 		3178.7
(-0.3)
P 		3143.1
(-0.6)
P 		3560.5
(3)
P 		2946.3
(-2.2)
P 		2857
(-3)
P 		704
B 3358.1
(2.1)
P 		3314.1
(1.8)
P 		3492.4
(3)
P 		3447.2
(2.7)
P 		2610.1
(-3)
P 		2685.8
(-2.5)
P 		882
C 3350.4
(-0.3)
P 		3052.5
(-1.8)
P 		3993.3
(3)
P 		3269.9
(-0.7)
P 		2835.9
(-2.9)
P 		2812.9
(-3)
P 		1180
avr 3290.5
(1) 		3181.8
(0.1) 		3542.9
(3) 		3425.9
(2.1) 		2797.4
(-2.9) 		2785.2
(-3) 		758
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1466
1 2 3 4 range
A 1251
(2.7)
P 		1020.3
(1.4)
P 		1316.8
(3)
P 		182.1
(-3)
P 		1135
B 990.3
(2.7)
P 		953.9
(2.4)
P 		1017.9
(3)
P 		368.3
(-3)
P 		650
C 1041.2
(0.5)
P 		1080.4
(0.6)
P 		1648.5
(3)
P 		217
(-3)
P 		1432
avr 1094.2
(1.7) 		1018.2
(1.3) 		1327.7
(3) 		255.8
(-3) 		1072

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 659
1 2 3 4 5 6 range
A 583.1
(-3)
P 		1130.5
(3)
P 		684.5
(-1.9)
P 		903
(0.5)
P 		889.1
(0.4)
P 		695.6
(-1.8)
P 		547
B 1168.4
(3)
P 		1103.1
(1.8)
P 		848.1
(-3)
P 		913
(-1.8)
P 		1060.5
(1)
P 		1001.5
(-0.1)
P 		320
C 1241.8
(3)
P 		931.4
(-0.2)
P 		1016.4
(0.7)
P 		657.6
(-3)
P 		917.6
(-0.3)
P 		941.6
(-0.1)
P 		584
avr 997.8
(1.5) 		1055
(3) 		849.7
(-2.3) 		824.5
(-3) 		955.7
(0.4) 		879.6
(-1.6) 		231
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175991_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6124
Sequence Type Exemplar sequence
Representative Public ID ZK686.3 NCBI
Target Description ZK686.3 /REP_DB=WormBase Gene ID /WP=CE00457 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066290(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066290 NCBI Caenorhabditis elegans ZK686.3 (ZK686.3) mRNA, complete cds. 11/11 None
SNAP00000038836 ENSEMBL cdna:SNAP chromosome:CEL140:III:7767738:7768986:-1 11/11 None
GENEFINDER00000038844 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7767738:7768986:-1 11/11 None
ZK686.3 ENSEMBL cdna:known chromosome:CEL140:III:7767494:7768986:-1 gene:ZK686.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7767735-7768957(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl ZK686.3
Entrez Gene 176089 Entrez gene
SwissProt P34669 EMBL-EBI
WormBase CE00457 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1587369_AT similar to tumor suppressor candidate 3 isoform b cfa
CANINE_2:CFA.1243.1.A1_AT similar to tumor suppressor candidate 3 isoform b cfa
CANINE_2:CFA.17390.1.S1_S_AT similar to tumor suppressor candidate 3 isoform b cfa
CANINE_2:CFAAFFX.11143.1.S1_AT similar to tumor suppressor candidate 3 isoform b cfa
DROSOPHILA_2:1634945_AT dm
DROSGENOME1:152401_AT dm
CHICKEN:GGA.12250.1.S1_AT tumor suppressor candidate 3 gga
HG-U133_PLUS_2:213423_X_AT tumor suppressor candidate 3 hs
HG-U133A_2:213423_X_AT tumor suppressor candidate 3 hs
HG-U133A:213423_X_AT tumor suppressor candidate 3 hs
HG-U133_PLUS_2:232770_AT tumor suppressor candidate 3 hs
HG-U133B:232770_AT tumor suppressor candidate 3 hs
HG-U133A:209228_X_AT tumor suppressor candidate 3 hs
HG-U133_PLUS_2:209228_X_AT tumor suppressor candidate 3 hs
HG-U133A_2:209228_X_AT tumor suppressor candidate 3 hs
HG-U95AV2:36852_AT tumor suppressor candidate 3 hs
HUGENEFL:U42359_AT tumor suppressor candidate 3 hs
HUGENEFL:U42360_CDS2_AT tumor suppressor candidate 3 hs
U133_X3P:HS.71119.1.A1_3P_A_AT tumor suppressor candidate 3 hs
U133_X3P:HS.71119.0.A2_3P_AT tumor suppressor candidate 3 hs
U133_X3P:HS.255425.0.S1_3P_AT tumor suppressor candidate 3 hs
HG-U133A_2:209227_AT tumor suppressor candidate 3 hs
HG-FOCUS:209227_AT tumor suppressor candidate 3 hs
HG-U133_PLUS_2:209227_AT tumor suppressor candidate 3 hs
HG-U133A:209227_AT tumor suppressor candidate 3 hs
HG-U95AV2:36850_AT tumor suppressor candidate 3 hs
HG-U95D:72839_AT Tumor suppressor candidate 3 hs
HU35KSUBC:RC_AA136469_AT Tumor suppressor candidate 3 hs
HG-U95AV2:36851_G_AT Tumor suppressor candidate 3 hs
HG-U133_PLUS_2:235801_AT Tumor suppressor candidate 3 hs
HG-U133B:235801_AT Tumor suppressor candidate 3 hs
HG-U95E:76622_AT Tumor suppressor candidate 3 hs
HG-U95D:80015_AT Tumor suppressor candidate 3 hs
HG-U95E:81131_AT Tumor suppressor candidate 3 hs
HG-U95D:84692_AT Tumor suppressor candidate 3 hs
U133_X3P:HS.283526.0.A1_3P_AT Tumor suppressor candidate 3 hs
MOUSE430_2:1421662_A_AT tumor suppressor candidate 3 mm
MOE430A:1421662_A_AT tumor suppressor candidate 3 mm
MOUSE430A_2:1421662_A_AT tumor suppressor candidate 3 mm
MG-U74BV2:108025_AT tumor suppressor candidate 3 mm
MG-U74CV2:140694_AT Tumor suppressor candidate 3, mRNA (cDNA clone IMAGE:3154952) mm
MOE430B:1458809_AT Tumor suppressor candidate 3, mRNA (cDNA clone IMAGE:3154952) mm
MOUSE430_2:1458809_AT Tumor suppressor candidate 3, mRNA (cDNA clone IMAGE:3154952) mm
MG-U74CV2:170246_AT Tumor suppressor candidate 3, mRNA (cDNA clone IMAGE:3154952) mm
RAE230A:1390419_A_AT similar to N33 protein rn
RAT230_2:1390419_A_AT similar to N33 protein rn
YEAST_2:1777628_AT Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins Sc
Protein Similarities
Method ID Description E-Value
blast AAA28222 Hypothetical protein ZK686.3 [Caenorhabditis elegans] ref|NP_498691.1| ZK686.3 [Caenorhabditis elegans] sp|P34669|YO13_CAEEL Hypothetical protein ZK686.3 in chromosome III 0.0
blast CAE57187 Hypothetical protein CBG00031 [Caenorhabditis briggsae] 1.0E-166
blast CAE57187 Hypothetical protein CBG00031 [Caenorhabditis briggsae] 1.0E-164
blast AAH69898 Putative prostate cancer tumor suppressor [Mus musculus] dbj|BAE21346.1| unnamed protein product [Mus musculus] dbj|BAC40455.1| unnamed protein product [Mus musculus] 8.0E-77
Protein Domains
Database ID Description E-Value
Pfam IPR006844 EMBL-EBI OST3/OST6 1.0E-126
Pfam IPR006844 EMBL-EBI OST3/OST6 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_498691.1 4 166-185,192-214,245-267,288-310
ZK686.3 4 166-185,192-214,245-267,288-310
SNAP00000038836 4 175-194,201-223,254-276,297-319
GENEFINDER00000038844 4 175-194,201-223,254-276,297-319
Sequence
Target Sequence 
>C. ELEGANS:175991_S_AT
gaagttcatgttcgtgttatccgcccaccaaactatactgctccagtcgtaattgctctt
tttgttgcactgctccttggaatgttgtacatgaagagaaacagtttggacttcctcttc
aataggactgtgtggggatttgtctgcctcgctatcactttcatcttcatgtccggacaa
atgtggaatcatattcgtggaccaccattcatgattaccaacccgaacaccaaagaacca
tcattcattcacggatccactcaattccaactcatcgctgaaacttacatcgtcggactt
ttgtatgctttgatcgctattggattcatttgtgtcaatgaagcagccgaccagtcgaac
agcaaagatagaaagaatgctggaaagaagctgaacccactttccttgttgaatatccct
acaaacactttggcaatcgctggacttgtgtgcatttgcgtcttct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAAGTTCATGTTCGTGTTATCCGCC 263 337 475 Antisense
ATACTGCTCCAGTCGTAATTGCTCT 680 23 509 Antisense
GTTGCACTGCTCCTTGGAATGTTGT 527 439 538 Antisense
GAAACAGTTTGGACTTCCTCTTCAA 682 355 572 Antisense
GACTGTGTGGGGATTTGTCTGCCTC 451 375 600 Antisense
ACTTTCATCTTCATGTCCGGACAAA 596 101 631 Antisense
GGACCACCATTCATGATTACCAACC 99 523 673 Antisense
GAACCATCATTCATTCACGGATCCA 684 345 709 Antisense
ATCGCTGAAACTTACATCGTCGGAC 461 37 748 Antisense
GAACCCACTTTCCTTGTTGAATATC 170 347 867 Antisense
GGACTTGTGTGCATTTGCGTCTTCT 622 525 916 Antisense
Sequence Source Affymetrix Proprietary Database