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[175988_at]

Description Y5H2B.4 /REP_DB=WormBase Gene ID /WP=CE21318 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 18      6grp_cgc > 45      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 9
1 2 3 4 range
A 3.1
(-0.7)
A 		4.1
(3)
A 		2.8
(-1.9)
A 		2.5
(-3)
A 		2
B 2.9
(1.6)
A 		1.9
(-3)
A 		2
(-2.5)
A 		3.2
(3)
A 		1
C 2.8
(-3)
A 		5.6
(-0.9)
A 		3.4
(-2.5)
A 		10.7
(3)
A 		8
avr 2.9
(-2.6) 		3.9
(-0.4) 		2.7
(-3) 		5.5
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 5
1 2 3 4 5 6 range
A 4.4
(3)
A 		1.4
(-3)
A 		1.7
(-2.4)
A 		3.3
(0.8)
A 		2
(-1.8)
A 		3.2
(0.6)
A 		3
B 1.4
(-2.6)
A 		1.6
(-2.2)
A 		4.1
(3)
A 		1.3
(-2.8)
A 		3.4
(1.6)
A 		1.2
(-3)
A 		3
C 1.6
(-2.4)
A 		5.8
(3)
A 		2.3
(-1.5)
A 		2.6
(-1.1)
A 		1.1
(-3)
A 		4.1
(0.8)
A 		5
avr 2.5
(-0.4) 		2.9
(3) 		2.7
(1.3) 		2.4
(-1.3) 		2.2
(-3) 		2.8
(2.1) 		1
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 1.1
(3)
A 		0.9
(1.3)
A 		0.7
(-0.4)
A 		0.4
(-3)
A 		1
B 0.9
(3)
A 		0.5
(-3)
A 		0.5
(-3)
A 		0.7
(-0)
A 		0
C 0.9
(-3)
A 		1.6
(1.7)
A 		1.2
(-1)
A 		1.8
(3)
A 		1
avr 1
(3) 		1
(3) 		0.8
(-3) 		1
(3) 		0

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2
1 2 3 4 5 6 range
A 0.8
(3)
A 		0.5
(-1.5)
A 		0.4
(-3)
A 		0.8
(3)
A 		0.6
(-0)
A 		0.8
(3)
A 		0
B 0.5
(-1.9)
A 		0.5
(-1.9)
A 		1
(0.8)
A 		0.3
(-3)
A 		1.4
(3)
A 		0.4
(-2.5)
A 		1
C 0.6
(-2.1)
A 		1.8
(3)
A 		0.6
(-2.1)
A 		0.5
(-2.6)
A 		0.4
(-3)
A 		1.4
(1.3)
A 		1
avr 0.6
(-1.5) 		0.9
(3) 		0.7
(-0) 		0.5
(-3) 		0.8
(1.5) 		0.9
(3) 		0
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175988_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.15543
Sequence Type Exemplar sequence
Representative Public ID Y5H2B.4 NCBI
Target Description Y5H2B.4 /REP_DB=WormBase Gene ID /WP=CE21318 /SUBMIT=ST.LOUIS /CHR=5 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_071195(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_071195 NCBI Caenorhabditis elegans Serpentine Receptor, class BC (class B-like) family member (srbc-42) (srbc-42) mRNA, complete cds. 11/11 None
GENEFINDER00000028133 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:2189273:2191050:-1 11/11 None
Y5H2B.4 ENSEMBL cdna:known chromosome:CEL140:V:2189414:2191050:-1 gene:Y5H2B.4 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000028126 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:2189413-2191050(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol srbc-42
Ensembl Y5H2B.4
Entrez Gene 189363 Entrez gene
SwissProt Q9N4Q8 EMBL-EBI
WormBase CE38124 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_503596 Serpentine Receptor, class BC (class B-like) family member (srbc-42) [Caenorhabditis elegans] gb|AAF59631.2| Hypothetical protein Y5H2B.4 [Caenorhabditis elegans] 1.0E-152
blast NP_503596 Serpentine Receptor, class BC (class B-like) family member (srbc-42) [Caenorhabditis elegans] gb|AAF59631.2| Hypothetical protein Y5H2B.4 [Caenorhabditis elegans] 1.0E-150
blast AAC25876 Serpentine receptor, class bc (class b-like) protein 24 [Caenorhabditis elegans] ref|NP_503582.1| Serpentine Receptor, class BC (class B-like) family member (srbc-24) [Caenorhabditis elegans] 2.0E-59
blast CAE74165 Hypothetical protein CBG21838 [Caenorhabditis briggsae] 4.0E-58
blast AAC25876 Serpentine receptor, class bc (class b-like) protein 24 [Caenorhabditis elegans] ref|NP_503582.1| Serpentine Receptor, class BC (class B-like) family member (srbc-24) [Caenorhabditis elegans] 2.0E-57
blast CAE74165 Hypothetical protein CBG21838 [Caenorhabditis briggsae] 1.0E-56
Protein Domains
Database ID Description E-Value
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 4.2E-76
Pfam IPR002892 EMBL-EBI Protein of unknown function DUF40 4.2E-76
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_503596.2 6 10-32,52-74,125-147,154-176,180-202,215-233
Y5H2B.4 6 10-32,52-74,125-147,154-176,180-202,215-233
GENEFINDER00000028133 7 9-31,62-84,93-115,135-157,164-186,190-212,225-243
Sequence
Target Sequence 
>C. ELEGANS:175988_AT
gttagccgatcacttattactttagcaatttcaattgaaagatttgtcgccgtttatttc
ccaatattattcctccaaaaacatccattcttcccgaactcggtgattccgatcattgca
atcggctacggtgtcttcgagtatccagttttgtatagtttttgcaattttgaactcaat
attccatacggatgcgtaactcttagctgctcactgaatttatgcttttatcaatattgg
acaacttataaaacgatcgtgtatattctgacttttttatccacgattgttttgacctgt
aagcttttgctgaaaatccacgaaagtcagagcaacaatttgactcgagcgaatcgactt
gcactcatcgactgtattgtcatatttttgtttgattttttcccaatattatttgaaaaa
ctagtcactcagaaaaatatattcactcctcggaatttgggctcctacagcaccgtcacc
aaactgagcggttgcgct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTAGCCGATCACTTATTACTTTAG 219 447 328 Antisense
CTCCAAAAACATCCATTCTTCCCGA 531 233 400 Antisense
TTCTTCCCGAACTCGGTGATTCCGA 702 687 415 Antisense
TTGCAATCGGCTACGGTGTCTTCGA 355 703 443 Antisense
GTGTCTTCGAGTATCCAGTTTTGTA 364 485 458 Antisense
TGCGTAACTCTTAGCTGCTCACTGA 553 583 520 Antisense
TTATCCACGATTGTTTTGACCTGTA 8 671 604 Antisense
GACTCGAGCGAATCGACTTGCACTC 534 373 669 Antisense
GACTTGCACTCATCGACTGTATTGT 354 371 683 Antisense
TATTCACTCCTCGGAATTTGGGCTC 421 663 767 Antisense
CGTCACCAAACTGAGCGGTTGCGCT 137 241 801 Antisense
Sequence Source Affymetrix Proprietary Database