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[175972_at]

Description E01A2.1 /REP_DB=WormBase Gene ID /WP=CE24869 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 37      6grp_high1_10_10 > 73      4grp_highest_8_8 > 09      4grp_high_8_8 > 53      6grp_highest_nonorm > 65      6grp_total > 63      4grp_total > 58      6grp_highest_sub > 03_04      6grp_high1_sub > 03_04      6grp_total_sub > Hat      4grp_rule_based > L3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 565
1 2 3 4 range
A 837.8
(-0.1)
P 		1064.5
(3)
P 		1051.1
(2.8)
P 		630.7
(-3)
P 		434
B 908.2
(-0.3)
P 		1143.9
(2.4)
P 		1195.9
(3)
P 		680
(-3)
P 		516
C 968.9
(-0.1)
P 		1120.7
(2.6)
P 		1143.7
(3)
P 		801.6
(-3)
P 		342
avr 905
(-0.2) 		1109.7
(2.7) 		1130.2
(3) 		704.1
(-3) 		426

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 547
1 2 3 4 5 6 range
A 1009.3
(-1.9)
P 		1001.5
(-2.1)
P 		1309
(3)
P 		1173.7
(0.8)
P 		1231.2
(1.7)
P 		943.8
(-3)
P 		365
B 787.5
(-3)
P 		903.5
(-1.7)
P 		1331.4
(3)
P 		994.4
(-0.7)
P 		1005.9
(-0.6)
P 		978.3
(-0.9)
P 		544
C 831.5
(-3)
P 		1064.4
(-0.2)
P 		1086.5
(0)
P 		1334.3
(3)
P 		929.5
(-1.8)
P 		1218.8
(1.6)
P 		503
avr 876.1
(-3) 		989.8
(-1.1) 		1242.3
(3) 		1167.5
(1.8) 		1055.5
(-0.1) 		1047
(-0.2) 		366
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 306
1 2 3 4 range
A 305
(3)
P 		231.1
(0.9)
P 		274.8
(2.1)
P 		92.3
(-3)
P 		213
B 289.6
(3)
P 		282.5
(2.7)
P 		277.1
(2.5)
P 		144.9
(-3)
P 		145
C 320.7
(1.2)
P 		322.2
(1.3)
P 		398.6
(3)
P 		135.2
(-3)
P 		263
avr 305.1
(2.6) 		278.6
(1.8) 		316.8
(3) 		124.1
(-3) 		193

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 223
1 2 3 4 5 6 range
A 186.1
(-3)
P 		356.2
(2.5)
P 		285.1
(0.2)
P 		297.7
(0.6)
P 		371.5
(3)
P 		229.8
(-1.6)
P 		185
B 274
(-2.6)
P 		300.7
(-1.5)
P 		323.3
(-0.5)
P 		263.4
(-3)
P 		408.7
(3)
P 		364.8
(1.2)
P 		145
C 308.2
(-1.3)
P 		324.8
(-0.6)
P 		276.5
(-2.6)
P 		268.3
(-3)
P 		300.8
(-1.6)
P 		408
(3)
P 		140
avr 256.1
(-3) 		327.2
(1.1) 		295
(-0.8) 		276.5
(-1.8) 		360.3
(3) 		334.2
(1.5) 		104
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175972_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.2087
Sequence Type Exemplar sequence
Representative Public ID E01A2.1 NCBI
Target Description E01A2.1 /REP_DB=WormBase Gene ID /WP=CE24869 /SUBMIT=ST.LOUIS /CHR=1 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_058904(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_058904 NCBI Caenorhabditis elegans E01A2.1 (E01A2.1) mRNA, complete cds. 11/11 None
GENEFINDER00000008590 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:4140667:4142271:1 10/11 None
SNAP00000008540 ENSEMBL cdna:SNAP chromosome:CEL140:I:4140765:4144164:1 11/11 None
E01A2.1 ENSEMBL cdna:known chromosome:CEL140:I:4140650:4142321:1 gene:E01A2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:4140666-4142271(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl E01A2.1
Entrez Gene 171998 Entrez gene
SwissProt Q966L2 EMBL-EBI
WormBase CE24869 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1605503_AT similar to Glutamate--cysteine ligase regulatory subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS light chain) (Glutamate--cysteine ligase modifier subunit) cfa
CANINE_2:CFA.2376.1.A1_AT similar to Glutamate--cysteine ligase regulatory subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS light chain) (Glutamate--cysteine ligase modifier subunit) cfa
CANINE_2:CFAAFFX.30811.1.S1_S_AT similar to Glutamate--cysteine ligase regulatory subunit (Gamma-glutamylcysteine synthetase) (Gamma-ECS) (GCS light chain) (Glutamate--cysteine ligase modifier subunit) cfa
DROSOPHILA_2:1640552_AT Glutamate-cysteine ligase modifier subunit dm
DROSGENOME1:151897_AT Glutamate-cysteine ligase modifier subunit dm
CHICKEN:GGA.2525.1.S1_S_AT glutamate-cysteine ligase, modifier subunit gga
CHICKEN:GGAAFFX.12034.1.S1_AT glutamate-cysteine ligase, modifier subunit gga
HG-U133_PLUS_2:236140_AT glutamate-cysteine ligase, modifier subunit hs
HG-U133B:236140_AT glutamate-cysteine ligase, modifier subunit hs
HG-U133_PLUS_2:203925_AT glutamate-cysteine ligase, modifier subunit hs
HG-U133A:203925_AT glutamate-cysteine ligase, modifier subunit hs
HG-FOCUS:203925_AT glutamate-cysteine ligase, modifier subunit hs
HG-U133A_2:203925_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBA:L35546_AT glutamate-cysteine ligase, modifier subunit hs
HUGENEFL:L35546_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBA:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBB:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBC:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBD:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HUGENEFL:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HC-G110:HUM_ALU_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBD:RC_AA454177_I_AT glutamate-cysteine ligase, modifier subunit hs
HU35KSUBD:RC_AA454177_R_AT glutamate-cysteine ligase, modifier subunit hs
U133_X3P:G4504010_3P_AT glutamate-cysteine ligase, modifier subunit hs
U133_X3P:HS.193452.0.A1_3P_AT glutamate-cysteine ligase, modifier subunit hs
HG-U95AV2:33163_R_AT glutamate-cysteine ligase, modifier subunit hs
HG-U95E:90801_AT glutamate-cysteine ligase, modifier subunit hs
HG-U133_PLUS_2:234986_AT Glutamate-cysteine ligase, modifier subunit hs
HG-U133B:234986_AT Glutamate-cysteine ligase, modifier subunit hs
HU35KSUBA:RC_D60670_AT Glutamate-cysteine ligase, modifier subunit hs
U133_X3P:HS.315562.0.A1_3P_AT Glutamate-cysteine ligase, modifier subunit hs
HG-U95C:55923_AT Glutamate-cysteine ligase, modifier subunit hs
HG-U95E:91740_R_AT Glutamate-cysteine ligase, modifier subunit hs
MOE430A:1418627_AT glutamate-cysteine ligase , modifier subunit mm
MOUSE430A_2:1418627_AT glutamate-cysteine ligase , modifier subunit mm
MOUSE430_2:1418627_AT glutamate-cysteine ligase , modifier subunit mm
MU11KSUBB:MSA.27785.0_S_AT glutamate-cysteine ligase , modifier subunit mm
MG-U74AV2:160335_AT glutamate-cysteine ligase , modifier subunit mm
MG-U74BV2:112204_AT Glutamate-cysteine ligase , modifier subunit (Gclm), mRNA mm
MU11KSUBB:MSA.23872.0_AT Glutamate-cysteine ligase , modifier subunit (Gclm), mRNA mm
MU19KSUBA:TC17854_AT Glutamate-cysteine ligase , modifier subunit (Gclm), mRNA mm
MU19KSUBB:TC30785_AT Glutamate-cysteine ligase , modifier subunit (Gclm), mRNA mm
RG-U34A:S65555_G_AT glutamate cysteine ligase, modifier subunit rn
RG-U34A:RC_AI233261_I_AT glutamate cysteine ligase, modifier subunit rn
RG-U34A:S65555_AT glutamate cysteine ligase, modifier subunit rn
RAE230A:1370030_AT glutamate cysteine ligase, modifier subunit rn
RAT230_2:1370030_AT glutamate cysteine ligase, modifier subunit rn
RG-U34B:RC_AA964814_AT Glutamate cysteine ligase, modifier subunit rn
Protein Similarities
Method ID Description E-Value
blast AAK85458 Hypothetical protein E01A2.1 [Caenorhabditis elegans] ref|NP_491305.1| E01A2.1 [Caenorhabditis elegans] 1.0E-159
blast AAK85458 Hypothetical protein E01A2.1 [Caenorhabditis elegans] ref|NP_491305.1| E01A2.1 [Caenorhabditis elegans] 1.0E-156
blast CAE66715 Hypothetical protein CBG12061 [Caenorhabditis briggsae] 1.0E-150
blast CAE66715 Hypothetical protein CBG12061 [Caenorhabditis briggsae] 1.0E-147
blast AAK85458 Hypothetical protein E01A2.1 [Caenorhabditis elegans] ref|NP_491305.1| E01A2.1 [Caenorhabditis elegans] 1.0E-135
blast CAE66715 Hypothetical protein CBG12061 [Caenorhabditis briggsae] 1.0E-126
blast AAQ96653 glutamate-cysteine ligase modifier subunit [Branchiostoma belcheri tsingtaunese] 5.0E-31
Sequence
Target Sequence 
>C. ELEGANS:175972_AT
accatgaaggttttcctgtcggcttgggatgttcagcaaattgatcaagccgtgacaagt
ctgaaagagcagctgaaaacgaaggatattgaggttctcattttgtcattccctgaactt
gacctcatcgacggcgaatccgaagaggacgagaacaatcgatggtttgagaaggtgaag
cctttgtacacctacatggagaagctcgtcgagacatccgagatcgcgtcaattggagtt
tccgatttctctgctcgtcaacttaaaacaattatcgaccatttcgacgtgaagccgagt
atcaaccacgttagattggatggatgctgtcaagttcctccagagctgcaagcactggcg
aatgataatgatgtgcagcttctagttcacaacgatccaactccattcccaacaaataat
attttcaagactttctgtgagatcgacagtggatcccagaaagccgtttgcagcccgctc
ttcgaaacgacatggctctcccgatactcagtttgggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ACCATGAAGGTTTTCCTGTCGGCTT 277 87 262 Antisense
TGAGGTTCTCATTTTGTCATTCCCT 515 567 351 Antisense
TGACCTCATCGACGGCGAATCCGAA 188 569 381 Antisense
TCGTCGAGACATCCGAGATCGCGTC 542 605 467 Antisense
ATTGGAGTTTCCGATTTCTCTGCTC 305 3 493 Antisense
ATTTCTCTGCTCGTCAACTTAAAAC 584 15 506 Antisense
GAAGCCGAGTATCAACCACGTTAGA 489 331 552 Antisense
TCAAGTTCCTCCAGAGCTGCAAGCA 218 631 591 Antisense
GCAGCTTCTAGTTCACAACGATCCA 422 313 636 Antisense
AGCCCGCTCTTCGAAACGACATGGC 445 83 733 Antisense
GCTCTCCCGATACTCAGTTTGGGTG 391 299 756 Antisense
Sequence Source Affymetrix Proprietary Database