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[175970_at]

Description Y105E8B.V /REP_DB=WormBase Gene ID /WP=CE25164 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_highest_10_10 > 31      6grp_high1_10_10 > 13      4grp_highest_8_8 > 21      4grp_high_8_8 > 07      6grp_highest_nonorm > 41      6grp_total > 14      4grp_total > 0      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 1018.1
(-1.4)
P 		927.8
(-3)
P 		993.2
(-1.9)
P 		1272.9
(3)
P 		345
B 1051.4
(-2.2)
P 		1024.2
(-3)
P 		1186.8
(1.6)
P 		1235.7
(3)
P 		212
C 1033
(-2.4)
P 		1015.7
(-3)
P 		1165.9
(2.2)
P 		1187.5
(3)
P 		172
avr 1034.2
(-1.9) 		989.2
(-3) 		1115.3
(0.1) 		1232
(3) 		243

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 392
1 2 3 4 5 6 range
A 842.6
(1.5)
P 		940.8
(3)
P 		636.9
(-1.7)
P 		684.1
(-0.9)
P 		549.2
(-3)
P 		604.2
(-2.2)
P 		392
B 921.4
(3)
P 		828.5
(1.4)
P 		785.8
(0.7)
P 		766.6
(0.4)
P 		580.8
(-2.8)
P 		568.6
(-3)
P 		353
C 939.5
(3)
P 		834.4
(1.1)
P 		813.8
(0.7)
P 		745.3
(-0.5)
P 		610.7
(-3)
P 		625.6
(-2.7)
P 		329
avr 901.2
(3) 		867.9
(2.4) 		745.5
(0.1) 		732
(-0.2) 		580.2
(-3) 		599.5
(-2.6) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 220
1 2 3 4 range
A 370.7
(3)
P 		201.4
(-2.5)
P 		259.6
(-0.6)
P 		186.3
(-3)
P 		184
B 335.3
(3)
P 		253
(-3)
P 		275
(-1.4)
P 		263.3
(-2.2)
P 		82
C 342
(1.1)
P 		292
(-0.3)
P 		406.3
(3)
P 		200.2
(-3)
P 		206
avr 349.3
(3) 		248.8
(-1.5) 		313.6
(1.4) 		216.6
(-3) 		133

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 210
1 2 3 4 5 6 range
A 155.3
(-2.5)
P 		334.6
(3)
P 		138.7
(-3)
P 		173.5
(-1.9)
P 		165.7
(-2.2)
P 		147.1
(-2.7)
P 		196
B 320.6
(3)
P 		275.8
(0.9)
P 		190.8
(-3)
P 		203
(-2.4)
P 		236
(-0.9)
P 		212
(-2)
P 		130
C 348.2
(3)
P 		254.6
(0.2)
P 		207.1
(-1.3)
P 		149.9
(-3)
P 		197.6
(-1.6)
P 		209.4
(-1.2)
P 		198
avr 274.7
(2.3) 		288.3
(3) 		178.9
(-2.8) 		175.5
(-3) 		199.8
(-1.7) 		189.5
(-2.3) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175970_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18782
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.V NCBI
Target Description Y105E8B.V /REP_DB=WormBase Gene ID /WP=CE25164 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170951(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_170951 NCBI Caenorhabditis elegans Y105E8A.19 (Y105E8A.19) mRNA, complete cds. 11/11 None
SNAP00000013344 ENSEMBL cdna:SNAP chromosome:CEL140:I:14485037:14506409:-1 11/11 None
GENEFINDER00000013488 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14487406:14494868:-1 11/11 None
Y105E8A.19 ENSEMBL cdna:known chromosome:CEL140:I:14484792:14494880:-1 gene:Y105E8A.19 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14487336-14494799(-) 96.06 100.0
Public Domain and Genome References
Gene Title
Ensembl Y105E8A.19
Entrez Gene 173311 Entrez gene
SwissProt Q8WQA5 EMBL-EBI
WormBase CE33722 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATH1-121501:267446_S_AT tRNA synthetase class I (W and Y) family protein at
CANINE:1591677_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
CANINE_2:CFA.17812.1.S1_S_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
CANINE_2:CFA.19844.1.S1_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
CANINE_2:CFAAFFX.16411.1.S1_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
CANINE_2:CFAAFFX.16428.1.S1_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
CANINE_2:CFAAFFX.16428.1.S1_S_AT similar to Tyrosyl-tRNA synthetase, cytoplasmic (Tyrosyl--tRNA ligase) (TyrRS) cfa
DROSOPHILA_2:1632234_AT Tyrosyl-tRNA synthetase dm
DROSGENOME1:142649_AT Tyrosyl-tRNA synthetase dm
CHICKEN:GGA.4637.1.S1_S_AT tyrosyl-tRNA synthetase gga
CHICKEN:GGA.4637.2.S1_S_AT tyrosyl-tRNA synthetase gga
HG-U133A_2:212048_S_AT tyrosyl-tRNA synthetase hs
HG-FOCUS:212048_S_AT tyrosyl-tRNA synthetase hs
HG-U133_PLUS_2:212048_S_AT tyrosyl-tRNA synthetase hs
HG-U133A:212048_S_AT tyrosyl-tRNA synthetase hs
HU35KSUBA:AA232121_R_AT tyrosyl-tRNA synthetase hs
HU35KSUBA:AA232121_S_AT tyrosyl-tRNA synthetase hs
HU35KSUBA:RC_AA257977_AT tyrosyl-tRNA synthetase hs
HU35KSUBC:RC_AA102053_AT tyrosyl-tRNA synthetase hs
HUGENEFL:U40714_AT tyrosyl-tRNA synthetase hs
U133_X3P:HS.239307.1.A2_3P_A_AT tyrosyl-tRNA synthetase hs
U133_X3P:HS.168020.0.A1_3P_AT tyrosyl-tRNA synthetase hs
HG-U133_PLUS_2:238760_AT tyrosyl-tRNA synthetase hs
HG-U133B:238760_AT tyrosyl-tRNA synthetase hs
HG-U95AV2:38977_AT tyrosyl-tRNA synthetase hs
HG-U95B:43172_AT tyrosyl-tRNA synthetase hs
HG-U95D:71297_AT Tyrosyl-tRNA synthetase hs
HG-U95E:72663_AT Tyrosyl-tRNA synthetase hs
HG-U95E:84414_AT Tyrosyl-tRNA synthetase hs
HU35KSUBC:RC_AA146671_AT Tyrosyl-tRNA synthetase hs
HU35KSUBD:RC_R06129_AT Tyrosyl-tRNA synthetase hs
MU11KSUBA:AA673272_S_AT tyrosyl-tRNA synthetase mm
MU11KSUBB:MSA.16669.0_S_AT tyrosyl-tRNA synthetase mm
MU11KSUBB:MSA.18695.0_S_AT tyrosyl-tRNA synthetase mm
MG-U74AV2:93564_AT tyrosyl-tRNA synthetase mm
MOE430A:1460638_AT tyrosyl-tRNA synthetase mm
MOUSE430A_2:1460638_AT tyrosyl-tRNA synthetase mm
MOUSE430_2:1460638_AT tyrosyl-tRNA synthetase mm
MG-U74AV2:162093_AT Tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:37380 IMAGE:4976999) mm
MU11KSUBA:AA492894_AT Tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:37380 IMAGE:4976999) mm
MU19KSUBA:TC14996_AT Tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:37380 IMAGE:4976999) mm
MU19KSUBA:TC14996_G_AT Tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:37380 IMAGE:4976999) mm
MU19KSUBB:TC33433_AT Tyrosyl-tRNA synthetase, mRNA (cDNA clone MGC:37380 IMAGE:4976999) mm
RG-U34C:RC_AI235277_AT tyrosyl-tRNA synthetase (predicted) rn
RAE230A:1372009_AT tyrosyl-tRNA synthetase (predicted) rn
RAT230_2:1372009_AT tyrosyl-tRNA synthetase (predicted) rn
YEAST_2:1774768_AT Cytoplasmic tyrosyl-tRNA synthetase, class I aminoacyl-tRNA synthetase that aminoacylates tRNA(Tyr), required for cytoplasmic protein synthesis, interacts with positions 34 and 35 of the anticodon of tRNATyr Sc
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6418 tRNA aminoacylation for protein translation inferred from electronic annotation QuickGO AmiGO
6437 tyrosyl-tRNA aminoacylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4812 tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
4831 tyrosine-tRNA ligase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAD21668 Hypothetical protein Y105E8A.19 [Caenorhabditis elegans] ref|NP_740947.2| Y105E8A.19 [Caenorhabditis elegans] dbj|BAC76736.1| tyrosyl-tRNA synthetase (cytoplasmic) [Caenorhabditis elegans] 0.0
blast CAE72594 Hypothetical protein CBG19783 [Caenorhabditis briggsae] 0.0
blast BAC76732.1 methionyl-tRNA synthetase (mitochondrial) [Caenorhabditis elegans] 0.0
blast CAG32285 hypothetical protein [Gallus gallus] ref|NP_001006314.1| tyrosyl-tRNA synthetase [Gallus gallus] 2.0E-85
Protein Domains
Database ID Description E-Value
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.8E-56
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.8E-56
Pfam IPR002305 EMBL-EBI Aminoacyl-tRNA synthetase, class Ib 9.8E-56
Sequence
Target Sequence 
>C. ELEGANS:175970_AT
gagaaaggctacaatcactccacggactccactgagccagccatcccaccatcggaagct
ccaaaaattccatcgctgagctccgaacaaaagcaatggaaagatcaattgctgaatgga
aatgagattcttatcggtgaagagcttgatcgtgtgcttgccgatgtttcaccccaaaaa
ccgcttcatttgatgttcattgcacctggaaaaggacgttttcacttgggattcattgct
ccattgctcaaaatcaaagaaatctccgatgcaggtggtgttccagtcattgcaacaatt
cttgtttcaccacttgaagcattcctggataatgagaaggttgcgtgggcggctattcaa
tcgagagcagtctattttagagaaatgttcatttctttgattaaacatttgaagctggat
ggtctcgtgaaggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGAAAGGCTACAATCACTCCACGG 357 393 1039 Antisense
AAAAATTCCATCGCTGAGCTCCGAA 485 127 1101 Antisense
GAAGAGCTTGATCGTGTGCTTGCCG 417 339 1177 Antisense
TTCACCCCAAAAACCGCTTCATTTG 208 687 1206 Antisense
GCTTCATTTGATGTTCATTGCACCT 597 305 1221 Antisense
TTGGGATTCATTGCTCCATTGCTCA 281 709 1264 Antisense
AAATCTCCGATGCAGGTGGTGTTCC 659 117 1298 Antisense
GGTGGTGTTCCAGTCATTGCAACAA 446 499 1312 Antisense
AACAATTCTTGTTTCACCACTTGAA 205 135 1332 Antisense
TGCGTGGGCGGCTATTCAATCGAGA 406 579 1380 Antisense
GAAGCTGGATGGTCTCGTGAAGGTT 330 343 1449 Antisense
Sequence Source Affymetrix Proprietary Database