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[175965_at]

Description Y50D4C.I /REP_DB=WormBase Gene ID /WP=CE26549 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 57      4grp_total > 13      6grp_cgc > 38      6grp_cgc_sub > Da      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 69
1 2 3 4 range
A 14.7
(1.1)
A 		18.1
(3)
A 		7.2
(-2.9)
A 		7.1
(-3)
A 		11
B 37.1
(3)
A 		7.5
(-2.4)
A 		4.4
(-3)
A 		12
(-1.6)
A 		33
C 9.4
(-2.9)
A 		10.5
(-2.8)
A 		8.8
(-3)
A 		72.9
(3)
A 		64
avr 20.4
(0.4) 		12
(-1.7) 		6.8
(-3) 		30.7
(3) 		24

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 17
1 2 3 4 5 6 range
A 13.5
(0.2)
A 		6.3
(-2.8)
A 		5.7
(-3)
A 		8.9
(-1.7)
A 		6.7
(-2.6)
A 		20.5
(3)
A 		15
B 3.3
(-3)
A 		3.5
(-2.6)
A 		4.1
(-1.3)
A 		6.1
(3)
A 		4
(-1.5)
A 		4.9
(0.4)
A 		3
C 3.4
(-2.7)
A 		3.8
(-2.1)
A 		7.2
(3)
A 		3.2
(-3)
A 		3.7
(-2.3)
A 		3.7
(-2.3)
A 		4
avr 6.7
(-0.5) 		4.5
(-3) 		5.7
(-1.6) 		6.1
(-1.2) 		4.8
(-2.7) 		9.7
(3) 		5
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 5.4
(3)
A 		3.9
(1)
A 		1.9
(-1.8)
A 		1
(-3)
A 		4
B 11.8
(3)
A 		1.9
(-2.5)
A 		1
(-3)
A 		2.6
(-2.1)
A 		11
C 3.1
(-2.9)
A 		3
(-3)
A 		3.1
(-2.9)
A 		12.3
(3)
A 		9
avr 6.8
(3) 		2.9
(-1.9) 		2
(-3) 		5.3
(1.1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 4
1 2 3 4 5 6 range
A 2.5
(-1.1)
A 		2.2
(-1.5)
A 		1.3
(-3)
A 		2.3
(-1.4)
A 		2
(-1.9)
A 		5
(3)
A 		4
B 1.1
(-2.3)
A 		1.2
(-1.5)
A 		1
(-3)
A 		1.6
(1.5)
A 		1.6
(1.5)
A 		1.8
(3)
A 		1
C 1.2
(-0)
A 		1.2
(-0)
A 		1.8
(3)
A 		0.6
(-3)
A 		1.2
(-0)
A 		1.2
(-0)
A 		1
avr 1.6
(-2.1) 		1.5
(-2.5) 		1.4
(-3) 		1.5
(-2.5) 		1.6
(-2.1) 		2.7
(3) 		1
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175965_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19548
Sequence Type Exemplar sequence
Representative Public ID Y50D4C.I NCBI
Target Description Y50D4C.I /REP_DB=WormBase Gene ID /WP=CE26549 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070959(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070959 NCBI Caenorhabditis elegans UNCoordinated family member (unc-34) (unc-34) mRNA, complete cds. 11/11 None
SNAP00000044736 ENSEMBL cdna:SNAP chromosome:CEL140:V:992766:995220:1 9/11 None
GENEFINDER00000044744 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:992176:995234:1 9/11 None
Y50D4C.1a ENSEMBL cdna:known chromosome:CEL140:V:982364:995234:1 gene:Y50D4C.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_001029083 2/11 Cross Hyb Matching Probes None
GENEFINDER00000044737 2/11 Cross Hyb Matching Probes None
Y50D4C.1b 2/11 Cross Hyb Matching Probes None
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
190098 2 1 NM_070959 175965_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:984962-995234(+) 89.51 100.0
Public Domain and Genome References
Ensembl Y50D4C.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAO12398 Uncoordinated protein 34, isoform b [Caenorhabditis elegans] ref|NP_001024254.1| UNCoordinated family member (unc-34) [Caenorhabditis elegans] 2.0E-83
blast AAO12397 Uncoordinated protein 34, isoform a [Caenorhabditis elegans] ref|NP_503360.3| UNCoordinated family member (unc-34) [Caenorhabditis elegans] 2.0E-83
blast AAN33048 UNC-34 [Caenorhabditis elegans] 2.0E-83
blast AAO12397 Uncoordinated protein 34, isoform a [Caenorhabditis elegans] ref|NP_503360.3| UNCoordinated family member (unc-34) [Caenorhabditis elegans] 5.0E-49
blast AAN33048 UNC-34 [Caenorhabditis elegans] 5.0E-49
blast AAO12397 Uncoordinated protein 34, isoform a [Caenorhabditis elegans] ref|NP_503360.3| UNCoordinated family member (unc-34) [Caenorhabditis elegans] 9.0E-45
blast AAN33048 UNC-34 [Caenorhabditis elegans] 9.0E-45
Protein Domains
Database ID Description E-Value
Pfam IPR001960 EMBL-EBI WH1 3.8E-14
Sequence
Target Sequence 
>C. ELEGANS:175965_AT
gggttaaagctgccgctgcagagcccgagaagcctgctgcgaaaggaggtaaaaatattg
gaaaatgtatgctaaaacaatttccaagtgaaaaattatgtattcagtgggcaagcagcg
gtgaaaccggcaatctaatgtccgaattggaggctcaactaaataagcgaaaaatgacac
aagcgaaatcggatgcagttgattcgaaatcaaatacgagcaatggatcatcggatagcg
gatgtggtacggcgacgtcgacgaatggatgtggatcatcgaatggtggatcggttggat
cggcggccgccaaaaaatggtctgtttcagatgcaacgaagcccatggatagtccgaaga
cgcatagaaaacttccatccgcctcatcactattctcacaagaagattcatcatcatcat
catcatctacgctgacaccttcaaaaccaacaacaaatggaacgtcatcggcgattccga
atgatctactcgagagactccgcgctgacataatggtcgaaatgcgactggagatgaaca
agctcgagcaacgaatcgtcgaggccaacgatcgggccgtcga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGGTTAAAGCTGCCGCTGCAGAGCC 261 497 938 Antisense
GCCCGAGAAGCCTGCTGCGAAAGGA 50 287 960 Antisense
ATGTGGTACGGCGACGTCGACGAAT 116 27 1179 Antisense
TGGTGGATCGGTTGGATCGGCGGCC 547 553 1221 Antisense
GGCGGCCGCCAAAAAATGGTCTGTT 449 539 1239 Antisense
GCAACGAAGCCCATGGATAGTCCGA 519 317 1270 Antisense
GACGCATAGAAAACTTCCATCCGCC 230 381 1296 Antisense
GCCTCATCACTATTCTCACAAGAAG 94 283 1318 Antisense
TCATCTACGCTGACACCTTCAAAAC 514 619 1360 Antisense
AATGATCTACTCGAGAGACTCCGCG 121 165 1417 Antisense
TCGAGGCCAACGATCGGGCCGTCGA 11 601 1496 Antisense
Sequence Source Affymetrix Proprietary Database