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[175963_at]

Description Y67D8A.G /REP_DB=WormBase Gene ID /WP=CE26229 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 62      4grp_high_8_8 > 18      6grp_highest_nonorm > 60      6grp_total > 17      4grp_total > 30      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 373
1 2 3 4 range
A 568.1
(2.1)
P 		398.6
(-2.9)
P 		394.2
(-3)
P 		600.5
(3)
P 		206
B 483.6
(3)
P 		403.3
(1.1)
P 		227.5
(-3)
P 		263.2
(-2.2)
P 		256
C 491.4
(2.5)
P 		339.4
(-0.9)
P 		241.6
(-3)
P 		515.2
(3)
P 		274
avr 514.4
(3) 		380.4
(-0.5) 		287.8
(-3) 		459.6
(1.5) 		227

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 536
1 2 3 4 5 6 range
A 784.2
(3)
P 		345.7
(-2.4)
P 		403.7
(-1.7)
P 		300.3
(-3)
P 		397.7
(-1.8)
P 		408.2
(-1.7)
P 		484
B 630.2
(3)
P 		440.2
(0)
P 		258
(-2.8)
P 		425.1
(-0.2)
P 		325.8
(-1.8)
P 		248
(-3)
P 		382
C 398.6
(0.8)
P 		458
(3)
P 		333.9
(-1.7)
P 		316.5
(-2.3)
P 		298.9
(-3)
P 		350.8
(-1)
P 		159
avr 604.3
(3) 		414.6
(-1.2) 		331.9
(-3) 		347.3
(-2.7) 		340.8
(-2.8) 		335.7
(-2.9) 		272
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 154
1 2 3 4 range
A 206.8
(3)
P 		86.5
(-3)
P 		103.1
(-2.2)
P 		87.9
(-2.9)
P 		120
B 154.2
(3)
P 		99.6
(-0.2)
P 		52.7
(-3)
P 		56.1
(-2.8)
P 		102
C 162.7
(3)
P 		97.6
(-2)
P 		84.2
(-3)
P 		86.9
(-2.8)
P 		79
avr 174.6
(3) 		94.6
(-1.9) 		80
(-2.8) 		77
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 157
1 2 3 4 5 6 range
A 144.6
(3)
P 		123
(1.1)
P 		87.9
(-2)
P 		76.2
(-3)
P 		120
(0.8)
P 		99.4
(-1)
P 		68
B 219.3
(3)
P 		146.5
(0.2)
P 		62.7
(-3)
P 		112.6
(-1.1)
P 		132.4
(-0.3)
P 		92.5
(-1.9)
P 		157
C 147.8
(3)
P 		139.7
(2.4)
P 		85
(-1.5)
P 		63.6
(-3)
P 		96.7
(-0.6)
P 		117.4
(0.8)
P 		84
avr 170.6
(3) 		136.4
(0.8) 		78.5
(-3) 		84.1
(-2.6) 		116.4
(-0.5) 		103.1
(-1.4) 		92
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175963_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19323
Sequence Type Exemplar sequence
Representative Public ID Y67D8A.G NCBI
Target Description Y67D8A.G /REP_DB=WormBase Gene ID /WP=CE26229 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067903
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
Y67D8A.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:3225606:3252571:1 gene:Y67D8A.1 A 
Y67D8A.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:3225606:3252575:1 gene:Y67D8A.1 A 
SNAP00000035502 ENSEMBL cdna:SNAP chromosome:CEL140:IV:3251601:3251975:1 None
GENEFINDER00000035505 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3249029:3252457:1 None
NM_067903 NCBI Caenorhabditis elegans Y67D8A.1 (Y67D8A.1) mRNA, complete cds. A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3249034-3251865(+) 64.63 100.0
Public Domain and Genome References
Gene Title
Ensembl Y67D8A.1
Entrez Gene 177094 Entrez gene
SwissProt Q95XU2 EMBL-EBI
WormBase CE31660 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598099_AT similar to phosphorylase kinase beta cfa
CANINE_2:CFA.10856.1.S1_AT similar to phosphorylase kinase beta cfa
CANINE_2:CFAAFFX.15996.1.S1_AT similar to phosphorylase kinase beta cfa
DROSOPHILA_2:1625204_A_AT dm
DROSGENOME1:152396_AT dm
CHICKEN:GGAAFFX.2485.1.S1_S_AT phosphorylase kinase, beta gga
CHICKEN:GGAAFFX.2485.2.S1_AT phosphorylase kinase, beta gga
CHICKEN:GGAAFFX.2485.3.S1_S_AT phosphorylase kinase, beta gga
HG-U133_PLUS_2:202739_S_AT phosphorylase kinase, beta hs
HG-U133A:202739_S_AT phosphorylase kinase, beta hs
HG-FOCUS:202739_S_AT phosphorylase kinase, beta hs
HG-U133A_2:202739_S_AT phosphorylase kinase, beta hs
HUGENEFL:X84908_AT phosphorylase kinase, beta hs
U133_X3P:G4505782_3P_A_AT phosphorylase kinase, beta hs
U133_X3P:HS.78060.0.A1_3P_A_AT phosphorylase kinase, beta hs
U133_X3P:HS.269887.0.A1_3P_X_AT phosphorylase kinase, beta hs
U133_X3P:HS.269887.0.A1_3P_AT phosphorylase kinase, beta hs
HG-U133A_2:202738_S_AT phosphorylase kinase, beta hs
HG-U133_PLUS_2:202738_S_AT phosphorylase kinase, beta hs
HG-U133A:202738_S_AT phosphorylase kinase, beta hs
HG-U95AV2:37392_AT phosphorylase kinase, beta hs
HG-U133_PLUS_2:242248_AT Phosphorylase kinase, beta hs
HG-U133B:242248_AT Phosphorylase kinase, beta hs
HG-U95D:85900_R_AT Phosphorylase kinase, beta hs
HG-U133_PLUS_2:238601_AT Phosphorylase kinase, beta hs
HG-U133B:238601_AT Phosphorylase kinase, beta hs
HG-U95E:83040_AT Phosphorylase kinase, beta hs
U133_X3P:HS.148154.0.A1_3P_AT Phosphorylase kinase, beta hs
MU11KSUBB:MSA.40053.0_S_AT phosphorylase kinase beta mm
MU11KSUBB:MSA.24732.0_S_AT phosphorylase kinase beta mm
MG-U74BV2:109086_AT phosphorylase kinase beta mm
MOE430A:1434511_AT phosphorylase kinase beta mm
MOUSE430A_2:1434511_AT phosphorylase kinase beta mm
MOUSE430_2:1434511_AT phosphorylase kinase beta mm
MG-U74AV2:96722_AT phosphorylase kinase beta mm
MG-U74BV2:113682_AT Phosphorylase kinase beta (Phkb), mRNA mm
MOE430B:1442053_AT Phosphorylase kinase beta (Phkb), mRNA mm
MOUSE430_2:1442053_AT Phosphorylase kinase beta (Phkb), mRNA mm
MU19KSUBC:TC35952_AT Phosphorylase kinase beta (Phkb), mRNA mm
MU19KSUBC:TC35953_AT Phosphorylase kinase beta (Phkb), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5977 glycogen metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5516 calmodulin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68543 Hypothetical protein Y67D8A.1 [Caenorhabditis elegans] ref|NP_500304.2| Y67D8A.1 [Caenorhabditis elegans] 0.0
blast CAE59898 Hypothetical protein CBG03382 [Caenorhabditis briggsae] 0.0
blast AAA31448 phosphorylase kinase beta-subunit 1.0E-163
blast AAK68543 Hypothetical protein Y67D8A.1 [Caenorhabditis elegans] ref|NP_500304.2| Y67D8A.1 [Caenorhabditis elegans] 1.0E-33
Protein Domains
Database ID Description E-Value
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.2E-31
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 6.3E-37
Trans Membrane
ID Number Of Domains Domain Boundaries
GENEFINDER00000035505 1 47-64
Sequence
Target Sequence 
>C. ELEGANS:175963_AT
aataggctccgcctttatcttggttttttttttgggtttttcgcgaaacatttctcgttc
ggcaaatttttatgtcgacaaattcggcaaaattttcaattccggcaaattgcgatttgc
cgattttcggaaattttcaatttcgacaatttgccgaatttcaattttaatcggaaaaat
tgtattttttcattctaagaacgttcctgcatctattttgaaattgctgctccgtcttat
aaaaatttccgacaaattgatgttcggcaaacgtaaaatcggcaaaccggcagtttgccg
aacttgccgaatttgccgtagtgcaattgccgcccacctgctgctgcagtggttgagttc
atcctggccggtcgtcatttctcccaagtcatcaatatctttggcgaaggaacacccgaa
gaaaagacgcttcccagtcagcatcaccgtggaaaaatgccgaaaatcgcctatactggt
cgtgcgagtagtgagaagaagaaacgacacgtcggaggatcatcatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATAGGCTCCGCCTTTATCTTGGTT 435 171 153 Antisense
TTCGCGAAACATTTCTCGTTCGGCA 456 697 192 Antisense
AAGAACGTTCCTGCATCTATTTTGA 73 151 349 Antisense
TTGAAATTGCTGCTCCGTCTTATAA 402 703 370 Antisense
GAATTTGCCGTAGTGCAATTGCCGC 691 325 461 Antisense
TGCAGTGGTTGAGTTCATCCTGGCC 279 579 497 Antisense
GTCATTTCTCCCAAGTCATCAATAT 593 467 526 Antisense
TATCTTTGGCGAAGGAACACCCGAA 573 659 548 Antisense
TTCCCAGTCAGCATCACCGTGGAAA 67 699 583 Antisense
GAAAATCGCCTATACTGGTCGTGCG 410 353 614 Antisense
ACGACACGTCGGAGGATCATCATCA 212 91 656 Antisense
Sequence Source Affymetrix Proprietary Database