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[175951_s_at]

Description C29E4.5 /REP_DB=WormBase Gene ID /WP=CE25794 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_highest_10_10 > 44      6grp_high1_10_10 > 43      4grp_highest_8_8 > 07      4grp_high_8_8 > 21      6grp_highest_nonorm > 72      6grp_total > 10      4grp_total > 18      6grp_highest_sub > 02      6grp_high1_sub > 02      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 181
1 2 3 4 range
A 461.9
(-1)
P 		445.7
(-3)
P 		449.3
(-2.6)
P 		494.9
(3)
P 		49
B 456.3
(-3)
P 		557
(1.1)
P 		479.4
(-2.1)
P 		603
(3)
P 		147
C 422.1
(-3)
P 		444.2
(-1.5)
P 		456.7
(-0.7)
P 		513.4
(3)
P 		91
avr 446.8
(-3) 		482.3
(-0.6) 		461.8
(-2) 		537.1
(3) 		90

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 453.5
(-0.5)
P 		472.1
(3)
P 		442.1
(-2.6)
P 		454.9
(-0.2)
P 		439.8
(-3)
P 		458.6
(0.5)
P 		32
B 342.2
(-3)
P 		535.5
(2.5)
P 		442.6
(-0.1)
P 		552.7
(3)
P 		389.8
(-1.6)
P 		429.5
(-0.5)
P 		211
C 355.4
(-3)
P 		477.1
(3)
P 		467.7
(2.5)
P 		447
(1.5)
P 		403.6
(-0.6)
P 		412.2
(-0.2)
P 		122
avr 383.7
(-3) 		494.9
(3) 		450.8
(0.6) 		484.9
(2.5) 		411.1
(-1.5) 		433.4
(-0.3) 		111
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 96
1 2 3 4 range
A 168.2
(3)
P 		96.8
(-1.5)
P 		117.4
(-0.2)
P 		72.4
(-3)
P 		96
B 145.5
(3)
P 		137.6
(1.6)
P 		111.1
(-3)
P 		128.5
(0)
P 		34
C 139.7
(1.4)
P 		127.7
(0.4)
P 		159.2
(3)
P 		86.6
(-3)
P 		73
avr 151.1
(3) 		120.7
(-0.3) 		129.2
(0.6) 		95.8
(-3) 		55

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 95
1 2 3 4 5 6 range
A 83.6
(-3)
P 		167.9
(3)
P 		96.3
(-2.1)
P 		115.4
(-0.7)
P 		132.7
(0.5)
P 		111.6
(-1)
P 		84
B 119.1
(-2)
P 		178.2
(3)
P 		107.5
(-3)
P 		146.4
(0.3)
P 		158.4
(1.3)
P 		160.1
(1.5)
P 		71
C 131.7
(1.5)
P 		145.6
(3)
P 		119
(0.1)
P 		89.9
(-3)
P 		130.6
(1.4)
P 		138
(2.2)
P 		56
avr 111.5
(-2.6) 		163.9
(3) 		107.6
(-3) 		117.2
(-2) 		140.6
(0.5) 		136.6
(0.1) 		56
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175951_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7595
Sequence Type Exemplar sequence
Representative Public ID C29E4.5 NCBI
Target Description C29E4.5 /REP_DB=WormBase Gene ID /WP=CE25794 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026017(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026017 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-250) (tag-250) mRNA, complete cds. 11/11 None
SNAP00000010830 ENSEMBL cdna:SNAP chromosome:CEL140:III:7918202:7921989:-1 11/11 None
GENEFINDER00000010847 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:7918202:7924839:-1 11/11 None
C29E4.5 ENSEMBL cdna:known chromosome:CEL140:III:7917918:7924791:-1 gene:C29E4.5 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
176113 2 1 NM_001026017 173750_at (11), 175951_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:7918199-7924789(-) 100.0 100.0
Public Domain and Genome References
Ensembl C29E4.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27964 Temporarily assigned gene name protein 250, isoform a [Caenorhabditis elegans] ref|NP_001021188.1| Temporarily Assigned Gene name family member (tag-250) [Caenorhabditis elegans] sp|P34344|TG250_CAEEL Hypothetical protein tag-250 0.0
blast CAE66904 Hypothetical protein CBG12287 [Caenorhabditis briggsae] 0.0
blast AAY44021 Temporarily assigned gene name protein 250, isoform b [Caenorhabditis elegans] ref|NP_001021189.1| Temporarily Assigned Gene name family member (tag-250) [Caenorhabditis elegans] 4.0E-57
Protein Domains
Database ID Description E-Value
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.9E-11
Pfam IPR008191 EMBL-EBI Maternal tudor protein 4.0E-17
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.9E-11
Pfam IPR008191 EMBL-EBI Maternal tudor protein 4.0E-17
Pfam IPR008191 EMBL-EBI Maternal tudor protein 1.7E-4
Pfam IPR008191 EMBL-EBI Maternal tudor protein 2.8E-4
Pfam IPR008191 EMBL-EBI Maternal tudor protein 1.4E-5
Sequence
Target Sequence 
>C. ELEGANS:175951_S_AT
ggacatctcattacggcaactttcattagacccgatgcccgattatatgtatgcaaagtt
gaaagacgaatgtgctctgcctgattctcaactggaaagttctcctgattttggaggatt
ctacgcggcttttatcgatgatcgttgggaacgtgttcagtgtattcgtgcgagtaaaat
tgacaaacaagcctattgtgtatatcttctggatgtgggcgctttccaatacgtgcgaaa
agaagcaatgcgacgtctcaactccacgtcaccattcaaaaaaatgctgatgttcaagtg
taaaatcggaggaatcaaaccagttgctggaggagaagtttggagccgcgagtcacatga
agctgttcgagaattctttgaagcagcatgtggtgaacctgttgtggttgaaccaactca
gcctggatggtctcaatggaaacaattaaatgctccagctgttccaacctgtgaagctcg
tttatcttgttgtggtagagatattggtgactggctcatcgcgtgtggtttggctcttcc
aataaatgcaccaattccaagtcccaattcacagtcacttcttacttttgttcca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGACATCTCATTACGGCAACTTTCA 347 525 1179 Antisense
GCAACTTTCATTAGACCCGATGCCC 24 321 1194 Antisense
AAAGACGAATGTGCTCTGCCTGATT 479 123 1240 Antisense
AGGATTCTACGCGGCTTTTATCGAT 118 57 1293 Antisense
TGTGGGCGCTTTCCAATACGTGCGA 435 551 1392 Antisense
GGAGCCGCGAGTCACATGAAGCTGT 605 521 1520 Antisense
GAACCAACTCAGCCTGGATGGTCTC 507 345 1588 Antisense
CTCCAGCTGTTCCAACCTGTGAAGC 111 237 1631 Antisense
GAGATATTGGTGACTGGCTCATCGC 305 397 1676 Antisense
TAAATGCACCAATTCCAAGTCCCAA 330 639 1721 Antisense
ACAGTCACTTCTTACTTTTGTTCCA 178 107 1749 Antisense
Sequence Source Affymetrix Proprietary Database