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[175946_at]

Description F54D8.6 /REP_DB=WormBase Gene ID /WP=CE25904 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 46      4grp_total > 06      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 185
1 2 3 4 range
A 110.5
(-1.1)
A 		82.5
(-2.4)
A 		68.7
(-3)
A 		203.4
(3)
A 		135
B 163.1
(2.4)
A 		107.2
(-1.9)
A 		93.3
(-3)
A 		170.7
(3)
P 		77
C 154.7
(0.8)
M 		39.2
(-3)
A 		81.8
(-1.6)
A 		223.8
(3)
P 		185
avr 142.8
(0.2) 		76.3
(-3) 		81.3
(-2.8) 		199.3
(3) 		123

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 125.2
(2.9)
A 		64
(-1.1)
A 		40.8
(-2.6)
A 		127.4
(3)
P 		35.4
(-3)
A 		76.5
(-0.3)
A 		92
B 68.3
(-3)
A 		78.6
(-2.3)
A 		155.8
(3)
P 		98.9
(-0.9)
A 		94.2
(-1.2)
A 		133.1
(1.4)
M 		88
C 101.4
(0.1)
P 		145.7
(3)
A 		66.5
(-2.1)
A 		53.2
(-3)
A 		67.7
(-2.1)
A 		128.8
(1.9)
A 		93
avr 98.3
(1.1) 		96.1
(0.9) 		87.7
(-0.2) 		93.2
(0.5) 		65.8
(-3) 		112.8
(3) 		47
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 41
1 2 3 4 range
A 40.2
(3)
A 		17.9
(-3)
A 		17.9
(-3)
A 		29.8
(0.2)
A 		22
B 52
(3)
A 		26.5
(-2)
A 		21.6
(-3)
A 		36.4
(-0.1)
P 		30
C 51.2
(3)
M 		11.3
(-3)
A 		28.5
(-0.4)
A 		37.7
(1)
P 		40
avr 47.8
(3) 		18.6
(-3) 		22.7
(-2.2) 		34.6
(0.3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 41
1 2 3 4 5 6 range
A 23.1
(0.6)
A 		22.8
(0.6)
A 		8.9
(-3)
A 		32.3
(3)
P 		10.7
(-2.5)
A 		18.6
(-0.5)
A 		23
B 23.8
(-3)
A 		26.2
(-2.4)
A 		37.8
(0.3)
P 		26.2
(-2.4)
A 		38.3
(0.4)
A 		49.6
(3)
M 		26
C 37.6
(1.8)
P 		44.5
(3)
A 		16.9
(-1.9)
A 		10.7
(-3)
A 		21.9
(-1)
A 		43.1
(2.8)
A 		34
avr 28.2
(-0.4) 		31.2
(0.8) 		21.2
(-3) 		23.1
(-2.3) 		23.6
(-2.1) 		37.1
(3) 		16
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175946_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.8060
Sequence Type Exemplar sequence
Representative Public ID F54D8.6 NCBI
Target Description F54D8.6 /REP_DB=WormBase Gene ID /WP=CE25904 /SUBMIT=ST.LOUIS /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027434(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027434 NCBI Caenorhabditis elegans F54D8.6 (F54D8.6) mRNA, complete cds. 11/11 None
GENEFINDER00000022635 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:5101947:5106777:1 10/11 None
SNAP00000022627 ENSEMBL cdna:SNAP chromosome:CEL140:III:5102950:5106777:1 10/11 None
F54D8.6 ENSEMBL cdna:known chromosome:CEL140:III:5101944:5106912:1 gene:F54D8.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:5101942-5106776(+) 95.2 100.0
Public Domain and Genome References
Gene Title
Ensembl F54D8.6
Entrez Gene 175695 Entrez gene
SwissProt Q8MQ25 EMBL-EBI
WormBase CE31000 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM48533 Hypothetical protein F54D8.6 [Caenorhabditis elegans] ref|NP_001022605.1| F54D8.6 [Caenorhabditis elegans] 0.0
blast H88449 protein F54D8.1 [imported] - Caenorhabditis elegans 0.0
blast CAE60043 Hypothetical protein CBG03554 [Caenorhabditis briggsae] 7.0E-89
blast CAE60043 Hypothetical protein CBG03554 [Caenorhabditis briggsae] 9.0E-69
Sequence
Target Sequence 
>C. ELEGANS:175946_AT
ggaggtggaaggcgcggagttgatagaccaccaggatctggaatgcctggaatcgatcgg
ccacctggaggggatcgaatgggtgataagcaaagaagaggagcgagaggaggtgttaag
gaaagagaaagaaaacgaagaagagatgaattccagcgaggaggtggtggtggtggccga
gcggtggccgtgatggtggaagagattatggtggtcctccgcaaaaatctgggcgacgtg
atcgatatgatgattacgatcgatcgtatcaaggaacaagtggatatggacaagattatg
attcacaaacatatggagaacgtggttacgatggacattatcaaggacaagaggattatt
atggaagtcaagaaggatatggagaatataatcagcaacactacggtg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGGTGGAAGGCGCGGAGTTGATA 360 515 1804 Antisense
CGCGGAGTTGATAGACCACCAGGAT 315 251 1816 Antisense
GAATGCCTGGAATCGATCGGCCACC 50 331 1844 Antisense
ATCGGCCACCTGGAGGGGATCGAAT 178 35 1859 Antisense
GAATTCCAGCGAGGAGGTGGTGGTG 8 325 1951 Antisense
CCGAGCGGTGGCCGTGATGGTGGAA 661 261 1980 Antisense
GAAGAGATTATGGTGGTCCTCCGCA 418 339 2002 Antisense
GGTCCTCCGCAAAAATCTGGGCGAC 497 501 2016 Antisense
ATCTGGGCGACGTGATCGATATGAT 254 33 2030 Antisense
GGAGAACGTGGTTACGATGGACATT 567 519 2118 Antisense
GAATATAATCAGCAACACTACGGTG 450 325 2187 Antisense
Sequence Source Affymetrix Proprietary Database