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[175944_at]

Description Y105E8B.P /REP_DB=WormBase Gene ID /WP=CE25170 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster 6grp_total > 95      4grp_total > 09      6grp_total_sub > Increase      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 93
1 2 3 4 range
A 43.7
(-1.4)
A 		42.2
(-1.5)
A 		22.6
(-3)
A 		102.6
(3)
A 		80
B 34.2
(-1.8)
A 		24.4
(-3)
A 		38.9
(-1.3)
A 		75
(3)
P 		51
C 14.8
(-3)
A 		56.2
(-0.3)
A 		78.7
(1.1)
A 		107.5
(3)
A 		93
avr 30.9
(-3) 		40.9
(-2.1) 		46.7
(-1.5) 		95
(3) 		64

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 69
1 2 3 4 5 6 range
A 15.2
(-2.9)
A 		40.7
(-0.5)
A 		13.7
(-3)
A 		47.9
(0.1)
A 		35.9
(-1)
A 		79.5
(3)
A 		66
B 27.4
(0)
A 		44
(3)
A 		10.5
(-3)
A 		31.5
(0.8)
A 		34.8
(1.4)
A 		26
(-0.2)
A 		34
C 70
(3)
A 		21
(-3)
A 		26.9
(-2.3)
A 		61.5
(2)
A 		63.1
(2.2)
A 		27.3
(-2.2)
A 		49
avr 37.5
(1.1) 		35.2
(0.6) 		17
(-3) 		47
(3) 		44.6
(2.5) 		44.3
(2.5) 		30
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 23
1 2 3 4 range
A 15.9
(3)
A 		9.2
(-1)
A 		5.9
(-3)
A 		15
(2.5)
A 		10
B 10.9
(-0.1)
A 		6
(-3)
A 		9
(-1.2)
A 		16
(3)
P 		10
C 4.9
(-3)
A 		16.2
(0)
A 		27.4
(3)
A 		18.1
(0.5)
A 		23
avr 10.6
(-2.9) 		10.5
(-3) 		14.1
(0.7) 		16.4
(3) 		6

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 23
1 2 3 4 5 6 range
A 2.8
(-3)
A 		14.5
(1.3)
A 		3
(-2.9)
A 		12.2
(0.4)
A 		10.8
(-0.1)
A 		19.3
(3)
A 		17
B 9.6
(0.5)
A 		14.7
(3)
A 		2.5
(-3)
A 		8.3
(-0.1)
A 		14.2
(2.8)
A 		9.7
(0.5)
A 		12
C 25.9
(3)
A 		6.4
(-3)
A 		6.8
(-2.9)
A 		12.4
(-1.2)
A 		20.4
(1.3)
A 		9.1
(-2.2)
A 		20
avr 12.8
(1.7) 		11.9
(1.3) 		4.1
(-3) 		11
(0.8) 		15.1
(3) 		12.7
(1.7) 		11
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175944_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18799
Sequence Type Exemplar sequence
Representative Public ID Y105E8B.P NCBI
Target Description Y105E8B.P /REP_DB=WormBase Gene ID /WP=CE25170 /CHR=1 /FEA=Sanger Annotation /DEF=(HINXTON)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Consensus/Exemplar Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade C annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_170944
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
SNAP00000013331 ENSEMBL cdna:SNAP chromosome:CEL140:I:14443426:14466242:-1 11/11 None
GENEFINDER00000013501 ENSEMBL cdna:GeneFinder chromosome:CEL140:I:14445159:14463961:-1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_170944 8/11 Cross Hyb Matching Probes A 
Y105E8A.12 8/11 Cross Hyb Matching Probes A 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:14452456-14463892(-) 100.0 100.0
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAD21662 Hypothetical protein Y105E8A.12 [Caenorhabditis elegans] ref|NP_740940.1| Y105E8A.12 [Caenorhabditis elegans] 0.0
blast CAE64135 Hypothetical protein CBG08751 [Caenorhabditis briggsae] 0.0
blast AAC24161 Hypothetical protein C02E7.1 [Caenorhabditis elegans] ref|NP_504328.1| C02E7.1 [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A1A1_HUMAN (P05023) Sodium/potassium-transporting ATPase alpha-1 chain precursor (EC 3.6.3.9) (Sodium pump 1) (Na+/K+ ATPase 1) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 1.0E-25
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 5.3E-46
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 5.1E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.8E-6
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 5.6E-10
Pfam IPR006068 EMBL-EBI Cation transporting ATPase, C-terminal 4.2E-29
Pfam IPR008250 EMBL-EBI E1-E2 ATPase-associated region 3.3E-39
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 5.1E-8
Pfam IPR005834 EMBL-EBI Haloacid dehalogenase-like hydrolase 1.8E-6
Pfam IPR004014 EMBL-EBI Cation transporting ATPase, N-terminal 5.6E-10
Trans Membrane
ID Number Of Domains Domain Boundaries
SNAP00000013331 8 132-154,158-180,324-346,356-378,944-966,1009-1031,1044-1066,1081-1098
GENEFINDER00000013501 7 147-166,173-195,359-381,388-410,983-1005,1081-1103,1118-1135
Sequence
Target Sequence 
>C. ELEGANS:175944_AT
gcgactttccgatgacttcagaacttgtcgataacccggaagagggtcaatcgagattca
tgattttcacaaaaggagcgcccgaggtgatcctgggcaagtgctccaactatagacagg
gcaaggagctgaagactatcgatgagacttataggacagagtgtcaggccgcctgggaga
tgctcggcaacgagggtcgccgcgtgatcgcctttgctcaaaaaagcttcaacgccgatg
acagcaccaagttcacaggccaggagtacaacggagacctagtatttcttgggatggcag
ccattatggatcccccacgtcctgaaacggcagctgctattgaacagtgtaagatggccg
gggtgaaggtgttcatgatcaccggggatcatccgacgacggcgacggcgatcgcaaggc
agattggcttgatcgggtcgagt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GCGACTTTCCGATGACTTCAGAACT 589 289 1922 Antisense
AGCGCCCGAGGTGATCCTGGGCAAG 473 79 1998 Antisense
GTGTCAGGCCGCCTGGGAGATGCTC 655 485 2082 Antisense
GAGATGCTCGGCAACGAGGGTCGCC 161 395 2098 Antisense
TCAACGCCGATGACAGCACCAAGTT 45 631 2150 Antisense
AGCACCAAGTTCACAGGCCAGGAGT 472 77 2164 Antisense
GGATGGCAGCCATTATGGATCCCCC 66 515 2213 Antisense
ACGTCCTGAAACGGCAGCTGCTATT 177 95 2238 Antisense
TGATCACCGGGGATCATCCGACGAC 429 563 2297 Antisense
GACGGCGATCGCAAGGCAGATTGGC 250 377 2325 Antisense
GCAGATTGGCTTGATCGGGTCGAGT 173 313 2340 Antisense
Sequence Source Affymetrix Proprietary Database