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[175941_at]

Description Y50D4C.G /REP_DB=WormBase Gene ID /WP=CE22292 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 93      4grp_total > 50      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 4
1 2 3 4 range
A 3.8
(-0.6)
A 		5.7
(3)
A 		5.7
(3)
A 		2.5
(-3)
A 		3
B 2.4
(-2.3)
A 		3.1
(3)
A 		2.9
(1.5)
A 		2.3
(-3)
A 		1
C 1.9
(-3)
A 		3
(3)
A 		2
(-2.5)
A 		2.9
(2.5)
A 		1
avr 2.7
(-2.5) 		3.9
(3) 		3.5
(1.2) 		2.6
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 14
1 2 3 4 5 6 range
A 2.9
(-1.1)
A 		1.3
(-3)
A 		6.3
(3)
A 		1.6
(-2.6)
A 		4.3
(0.6)
A 		2.9
(-1.1)
A 		5
B 3.1
(-2.6)
A 		4.4
(-2)
A 		3.6
(-2.4)
A 		2.3
(-3)
A 		15.5
(3)
A 		2.3
(-3)
A 		13
C 1.7
(-3)
A 		3.9
(-0.1)
A 		1.9
(-2.7)
A 		5.2
(1.6)
A 		6.3
(3)
A 		3.6
(-0.5)
A 		5
avr 2.6
(-3) 		3.2
(-2.4) 		3.9
(-1.7) 		3
(-2.6) 		8.7
(3) 		2.9
(-2.7) 		6
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1
1 2 3 4 range
A 1.4
(2.5)
A 		1.2
(1.4)
A 		1.5
(3)
A 		0.4
(-3)
A 		1
B 0.8
(3)
A 		0.8
(3)
A 		0.7
(1)
A 		0.5
(-3)
A 		0
C 0.6
(-1.5)
A 		0.9
(3)
A 		0.7
(-0)
A 		0.5
(-3)
A 		0
avr 0.9
(1.8) 		1
(3) 		1
(3) 		0.5
(-3) 		1

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 6
1 2 3 4 5 6 range
A 0.5
(-2.4)
A 		0.4
(-3)
A 		1.4
(3)
A 		0.4
(-3)
A 		1.3
(2.4)
A 		0.7
(-1.2)
A 		1
B 1.1
(-2.5)
A 		1.5
(-2.1)
A 		0.9
(-2.7)
A 		0.6
(-3)
A 		6.3
(3)
A 		0.9
(-2.7)
A 		6
C 0.6
(-2.6)
A 		1.2
(-0.2)
A 		0.5
(-3)
A 		1
(-1)
A 		2
(3)
A 		1.2
(-0.2)
A 		2
avr 0.7
(-3) 		1
(-2.3) 		0.9
(-2.5) 		0.7
(-3) 		3.2
(3) 		0.9
(-2.5) 		3
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175941_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19494
Sequence Type Exemplar sequence
Representative Public ID Y50D4C.G NCBI
Target Description Y50D4C.G /REP_DB=WormBase Gene ID /WP=CE22292 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_070956(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_070956 NCBI Caenorhabditis elegans Y50D4C.2 (Y50D4C.2) mRNA, complete cds. 11/11 A 
SNAP00000044731 ENSEMBL cdna:SNAP chromosome:CEL140:V:936294:973311:1 11/11 None
GENEFINDER00000044745 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:936294:939058:1 11/11 None
Y50D4C.2 ENSEMBL cdna:known chromosome:CEL140:V:936228:939197:1 gene:Y50D4C.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:936227-939058(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl Y50D4C.2
Entrez Gene 178598 Entrez gene
SwissProt Q965R0 EMBL-EBI
WormBase CE22292 Wormbase
Functional Annotations
Gene Ontology
GO Molecular Function (view graph)
ID Description Evidence Links
4024 alcohol dehydrogenase activity, zinc-dependent inferred from electronic annotation QuickGO AmiGO
8270 zinc ion binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68507 Hypothetical protein Y50D4C.2 [Caenorhabditis elegans] ref|NP_503357.1| Y50D4C.2 [Caenorhabditis elegans] 0.0
blast AAF39899 Hypothetical protein H24K24.3a [Caenorhabditis elegans] ref|NP_741507.1| H24K24.3a [Caenorhabditis elegans] sp|Q17335|ADHX_CAEEL Alcohol dehydrogenase class III (Glutathione-dependent formaldehyde dehydrogenase) (FDH) (FALDH) gb|AAB03374.1| alcohol dehydrogenase 0.0
blast AAL32231 Hypothetical protein H24K24.3b [Caenorhabditis elegans] ref|NP_001024016.1| H24K24.3b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.039
Pfam IPR002085 EMBL-EBI Zinc-containing alcohol dehydrogenase superfamily 1.0E-126
Pfam IPR001611 EMBL-EBI Leucine-rich repeat 0.039
Pfam IPR002085 EMBL-EBI Zinc-containing alcohol dehydrogenase superfamily 1.0E-126
Pfam IPR002085 EMBL-EBI Zinc-containing alcohol dehydrogenase superfamily 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175941_AT
cgtcggaatcgacttgattgaatcgaaattcgaaaaggccaagatgttcggagccaccga
atgcattaatccgaagagtgtggagcttccggaaggaaagagcttccaatcgtggctcgt
ggagcaattcgacggagggtttgactacacttttgagtgtattggaaatgtgcacacaat
gcgtcaagccctcgaagccgctcacaaaggatggggagtctcgtgtatcatcggagtcgc
cggagccggccaagagatcgccacccgcccattccaacttgtcaccggacgcacgtggaa
gggaaccgcattcggcggatggaaatccgtggaaagtgtgccgagactcgtcgacgacta
catgaacaagaagctgctgatcgatgaattcattactcatcgctgtaacattgacgatat
caatgcggccttcgacgt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGTCGGAATCGACTTGATTGAATCG 161 247 1197 Antisense
AAGGCCAAGATGTTCGGAGCCACCG 136 161 1231 Antisense
AATGCATTAATCCGAAGAGTGTGGA 217 163 1256 Antisense
AAGAGTGTGGAGCTTCCGGAAGGAA 156 153 1270 Antisense
GGAGCTTCCGGAAGGAAAGAGCTTC 459 521 1278 Antisense
AGAGCTTCCAATCGTGGCTCGTGGA 704 69 1295 Antisense
CTTCCAATCGTGGCTCGTGGAGCAA 34 219 1299 Antisense
GTGGAGCAATTCGACGGAGGGTTTG 45 489 1315 Antisense
GCAATTCGACGGAGGGTTTGACTAC 164 317 1320 Antisense
TTGACGATATCAATGCGGCCTTCGA 487 703 1607 Antisense
ACGATATCAATGCGGCCTTCGACGT 353 91 1610 Antisense
Sequence Source Affymetrix Proprietary Database