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[175931_s_at] protein kinase /// ---

Description Y73B3A.F /REP_DB=WormBase Gene ID /WP=CE26574 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 97      4grp_total > 16      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 66
1 2 3 4 range
A 24.1
(-2.8)
A 		48.9
(-0)
A 		22.3
(-3)
A 		76.3
(3)
A 		54
B 49.7
(0.9)
M 		25.2
(-3)
A 		63.1
(3)
P 		38.3
(-0.9)
M 		38
C 30.8
(-3)
A 		48.3
(-1.2)
A 		47.2
(-1.3)
A 		87.9
(3)
A 		57
avr 34.9
(-3) 		40.8
(-1.9) 		44.2
(-1.3) 		67.5
(3) 		33

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 109
1 2 3 4 5 6 range
A 47.5
(-2)
A 		35.5
(-2.8)
A 		33.2
(-3)
A 		47.8
(-1.9)
A 		116.5
(3)
P 		116.4
(3)
P 		83
B 37.5
(-2.8)
A 		35.9
(-3)
A 		54.9
(-0.6)
A 		69.7
(1.3)
M 		82.9
(3)
P 		74.9
(2)
P 		47
C 30.7
(-3)
A 		48.7
(-2)
A 		61.5
(-1.3)
A 		54.3
(-1.7)
A 		77.9
(-0.4)
P 		139.9
(3)
P 		109
avr 38.6
(-3) 		40
(-2.9) 		49.9
(-2.1) 		57.3
(-1.4) 		92.4
(1.5) 		110.4
(3) 		72
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 11
1 2 3 4 range
A 8.8
(0.3)
A 		10.6
(2.3)
A 		5.8
(-3)
A 		11.2
(3)
A 		5
B 15.9
(3)
M 		6.2
(-3)
A 		14.6
(2.2)
P 		8.2
(-1.8)
M 		10
C 10.2
(-3)
A 		13.9
(0.5)
A 		16.5
(3)
A 		14.8
(1.4)
A 		6
avr 11.6
(1) 		10.2
(-3) 		12.3
(3) 		11.4
(0.4) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 40
1 2 3 4 5 6 range
A 8.8
(-2.7)
A 		12.6
(-1.8)
A 		7.2
(-3)
A 		12.1
(-2)
A 		35.2
(3)
P 		28.3
(1.5)
P 		28
B 13.1
(-2.7)
A 		12
(-3)
A 		13.3
(-2.6)
A 		18.5
(-1.2)
M 		33.7
(3)
P 		27.9
(1.4)
P 		22
C 11.4
(-2.9)
A 		14.9
(-2.3)
A 		15.6
(-2.2)
A 		10.9
(-3)
A 		25.2
(-0.6)
P 		46.8
(3)
P 		36
avr 11.1
(-3) 		13.2
(-2.5) 		12
(-2.8) 		13.8
(-2.3) 		31.4
(2.3) 		34.3
(3) 		23
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175931_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19598
Sequence Type Exemplar sequence
Representative Public ID Y73B3A.F NCBI
Target Description Y73B3A.F /REP_DB=WormBase Gene ID /WP=CE26574 /CHR=X /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062344(11), NM_075674(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062344 NCBI Caenorhabditis elegans C16A11.3 (C16A11.3) mRNA, complete cds. 11/11 A 
NM_075674 NCBI Caenorhabditis elegans Y73B3A.16 (Y73B3A.16) mRNA, complete cds. 11/11 None
SNAP00000042940 ENSEMBL cdna:SNAP chromosome:CEL140:II:4229545:4234317:1 11/11 None
GENEFINDER00000042949 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:4231379:4234317:1 11/11 None
GENEFINDER00000022346 ENSEMBL cdna:GeneFinder chromosome:CEL140:X:77429:80400:1 10/11 None
SNAP00000022303 ENSEMBL cdna:SNAP chromosome:CEL140:X:78429:80327:1 9/11 None
C16A11.3 ENSEMBL cdna:known chromosome:CEL140:II:4229545:4234610:1 gene:C16A11.3 11/11 A 
Y73B3A.16 ENSEMBL cdna:known chromosome:CEL140:X:78429:80400:1 gene:Y73B3A.16 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:4232482-4234316(+) 98.07 98.07
chrX:78587-80400(+) 100.0 100.0
Public Domain and Genome References
Gene Title protein kinase

Ensembl C16A11.3
Y73B3A.16
Entrez Gene 173756 Entrez gene
190634 Entrez gene
SwissProt O76581 EMBL-EBI
Q95XE7 EMBL-EBI
WormBase CE19353 Wormbase
CE32275 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6355 regulation of transcription, DNA-dependent inferred from electronic annotation QuickGO AmiGO
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3677 DNA binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK31409 Hypothetical protein C16A11.3 [Caenorhabditis elegans] ref|NP_494745.1| C16A11.3 [Caenorhabditis elegans] 0.0
blast AAK39370 Hypothetical protein Y73B3A.16 [Caenorhabditis elegans] ref|NP_508075.2| Y73B3A.16 [Caenorhabditis elegans] 1.0E-156
blast AAK31409 Hypothetical protein C16A11.3 [Caenorhabditis elegans] ref|NP_494745.1| C16A11.3 [Caenorhabditis elegans] 1.0E-148
blast AAK39370 Hypothetical protein Y73B3A.16 [Caenorhabditis elegans] ref|NP_508075.2| Y73B3A.16 [Caenorhabditis elegans] 1.0E-148
blast AAK39370 Hypothetical protein Y73B3A.16 [Caenorhabditis elegans] ref|NP_508075.2| Y73B3A.16 [Caenorhabditis elegans] 1.0E-147
Protein Families
Method ID Description E-Value
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 6.0E-16
hanks 3.1.5 CMCG Group; CMGC I Cyclin-dependent (CDKs) and close relatives; Cdk6 8.0E-18
Protein Domains
Database ID Description E-Value
Pfam IPR006570 EMBL-EBI SPK 2.1E-37
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-9
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Pfam IPR000719 EMBL-EBI Protein kinase 4.5E-6
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-33
Pfam IPR000719 EMBL-EBI Protein kinase 7.9E-9
Pfam IPR000719 EMBL-EBI Protein kinase 9.1E-35
Pfam IPR000719 EMBL-EBI Protein kinase 4.7E-16
Pfam IPR000719 EMBL-EBI Protein kinase 0.0015
Pfam IPR000719 EMBL-EBI Protein kinase 3.6E-25
Sequence
Target Sequence 
>C. ELEGANS:175931_S_AT
atgccggcgggaagcgaaatttcatttgaaattaacgcgattcgagttatgggaaaacag
ttgctggaggcgatggagaagctggaatctgtgaaaattgtgcacttggacctgaagccc
gaaaacatttgcttcttctcgtcctgtatcttcaagactactgtaactggaaatgtgtgc
tttattcagccatcgacattccacgtgtgtgtggtggatttcggaaatgctcggacgatt
cgtcaggataagccgccaaaatatgcacttgtccagactcaaaactacagagcacctgaa
atcttcttgggacttccgttttcagtgaaatcggatatctggtcgtttggttgtattctt
aatgattgttcaacaacctctgtcgtgtgccatgaagccgaagaaatcaaaaacacaaaa
attcggattaaaaacggcaatgtgtacatgaagcggaaatcgaaatcatcgtttgagccg
ccaaagccgcttcataagcaac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCCGGCGGGAAGCGAAATTTCAT 121 39 13 Antisense
AATTGTGCACTTGGACCTGAAGCCC 473 179 108 Antisense
AATGTGTGCTTTATTCAGCCATCGA 545 163 184 Antisense
GGACGATTCGTCAGGATAAGCCGCC 653 521 245 Antisense
AAGCCGCCAAAATATGCACTTGTCC 303 149 262 Antisense
GCACTTGTCCAGACTCAAAACTACA 451 315 277 Antisense
GAGCACCTGAAATCTTCTTGGGACT 10 391 302 Antisense
CTTCTTGGGACTTCCGTTTTCAGTG 136 221 315 Antisense
TATCTGGTCGTTTGGTTGTATTCTT 665 657 348 Antisense
TCGAAATCATCGTTTGAGCCGCCAA 384 601 472 Antisense
CCGCCAAAGCCGCTTCATAAGCAAC 40 263 490 Antisense
Sequence Source Affymetrix Proprietary Database