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[175916_at]

Description F54A3.3 /REP_DB=WormBase Gene ID /WP=CE25900 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_highest_8_8 > 22      4grp_high_8_8 > 07      6grp_total > 59      4grp_total > 01      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 198
1 2 3 4 range
A 131.8
(-2.1)
P 		112.5
(-3)
P 		136.6
(-1.8)
P 		237.8
(3)
P 		125
B 129.2
(-2.6)
P 		115.1
(-3)
P 		138.2
(-2.3)
P 		310.6
(3)
P 		196
C 126.4
(-2)
P 		116.5
(-3)
P 		145.8
(0.1)
P 		173.4
(3)
P 		57
avr 129.1
(-2.3) 		114.7
(-3) 		140.2
(-1.8) 		240.6
(3) 		126

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 120
1 2 3 4 5 6 range
A 101.4
(-0.2)
P 		110.2
(0.6)
P 		68.8
(-3)
P 		68.7
(-3)
A 		90.8
(-1.1)
M 		138.1
(3)
P 		69
B 111.1
(3)
P 		75.3
(-0.9)
A 		56.5
(-3)
P 		102.9
(2.1)
A 		88.8
(0.5)
P 		103
(2.1)
P 		55
C 117.2
(1)
P 		106.5
(0.5)
A 		120
(1.1)
P 		37.1
(-3)
A 		102.7
(0.3)
P 		157
(3)
P 		120
avr 109.9
(0.8) 		97.3
(-0.4) 		81.8
(-1.8) 		69.6
(-3) 		94.1
(-0.7) 		132.7
(3) 		63
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 42
1 2 3 4 range
A 48
(3)
P 		24.4
(-3)
P 		35.7
(-0.1)
P 		34.8
(-0.4)
P 		24
B 41.2
(-1)
P 		28.4
(-3)
P 		32
(-2.4)
P 		66.2
(3)
P 		38
C 41.8
(0.5)
P 		33.5
(-1.8)
P 		50.8
(3)
P 		29.2
(-3)
P 		22
avr 43.7
(3) 		28.8
(-3) 		39.5
(1.3) 		43.4
(2.9) 		15

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 45
1 2 3 4 5 6 range
A 18.7
(-2.1)
P 		39.2
(3)
P 		15
(-3)
P 		17.4
(-2.4)
A 		27.4
(0.1)
M 		33.6
(1.6)
P 		24
B 38.7
(3)
P 		25.1
(-0.3)
A 		13.7
(-3)
P 		27.2
(0.2)
A 		36.1
(2.4)
P 		38.4
(2.9)
P 		25
C 43.4
(1.8)
P 		32.5
(0.3)
A 		30.5
(0.1)
P 		7.5
(-3)
A 		33.2
(0.4)
P 		52.5
(3)
P 		45
avr 33.6
(1) 		32.3
(0.7) 		19.7
(-2.4) 		17.4
(-3) 		32.2
(0.7) 		41.5
(3) 		24
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175916_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4604
Sequence Type Exemplar sequence
Representative Public ID F54A3.3 NCBI
Target Description F54A3.3 /REP_DB=WormBase Gene ID /WP=CE25900 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061817(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061817 NCBI Caenorhabditis elegans F54A3.3 (F54A3.3) mRNA, complete cds. 11/11 None
GENEFINDER00000020158 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:2233550:2242579:1 11/11 None
F54A3.3 ENSEMBL cdna:known chromosome:CEL140:II:2233541:2243540:1 gene:F54A3.3 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000022350 1/11 Cross Hyb Matching Probes None
SNAP00000022325 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2233548-2242578(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F54A3.3
Entrez Gene 173581 Entrez gene
SwissProt Q9N4J8 EMBL-EBI
WormBase CE31540 Wormbase
Functional Annotations
Ortholog
ID Title Organism
ATGENOME1:18906_AT chaperonin, putative at
ATH1-121501:246830_AT chaperonin, putative at
CANINE:1593613_AT similar to chaperonin containing TCP1, subunit 3 isoform c cfa
CANINE:1583379_AT similar to chaperonin containing TCP1, subunit 3 isoform c cfa
CANINE_2:CFA.5626.1.A1_AT similar to chaperonin containing TCP1, subunit 3 isoform c cfa
CANINE_2:CFAAFFX.7895.1.S1_S_AT similar to chaperonin containing TCP1, subunit 3 isoform c cfa
DROSOPHILA_2:1634782_S_AT dm
DROSGENOME1:153982_AT dm
HG-U95AV2:40774_AT chaperonin containing TCP1, subunit 3 (gamma) hs
HG-U133_PLUS_2:200910_AT chaperonin containing TCP1, subunit 3 (gamma) hs
HG-U133A:200910_AT chaperonin containing TCP1, subunit 3 (gamma) hs
HG-FOCUS:200910_AT chaperonin containing TCP1, subunit 3 (gamma) hs
HG-U133A_2:200910_AT chaperonin containing TCP1, subunit 3 (gamma) hs
HUGENEFL:X74801_AT chaperonin containing TCP1, subunit 3 (gamma) hs
U133_X3P:G5174726_3P_AT chaperonin containing TCP1, subunit 3 (gamma) hs
MOUSE430_2:1426067_X_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1426067_X_AT chaperonin subunit 3 (gamma) mm
MOE430A:1426067_X_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1451915_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1451915_AT chaperonin subunit 3 (gamma) mm
MOE430A:1451915_AT chaperonin subunit 3 (gamma) mm
MOE430A:1448178_A_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1448178_A_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1448178_A_AT chaperonin subunit 3 (gamma) mm
MOE430A:1416024_X_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1416024_X_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1416024_X_AT chaperonin subunit 3 (gamma) mm
MOE430A:1459987_S_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1459987_S_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1459987_S_AT chaperonin subunit 3 (gamma) mm
MU11KSUBA:C79428_RC_F_AT chaperonin subunit 3 (gamma) mm
MU11KSUBA:L20509_F_AT chaperonin subunit 3 (gamma) mm
MU11KSUBB:Z49085_S_AT chaperonin subunit 3 (gamma) mm
MG-U74AV2:98153_AT chaperonin subunit 3 (gamma) mm
MG-U74AV2:98446_S_AT chaperonin subunit 3 (gamma) mm
MG-U74AV2:161238_F_AT chaperonin subunit 3 (gamma) mm
MG-U74CV2:171548_AT chaperonin subunit 3 (gamma) mm
MOE430A:1449645_S_AT chaperonin subunit 3 (gamma) mm
MOUSE430A_2:1449645_S_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1449645_S_AT chaperonin subunit 3 (gamma) mm
MOE430B:1439679_AT chaperonin subunit 3 (gamma) mm
MOUSE430_2:1439679_AT chaperonin subunit 3 (gamma) mm
MU19KSUBB:TC33196_F_AT Chaperonin subunit 3 (gamma) (Cct3), mRNA mm
MU19KSUBC:TC39768_I_AT Chaperonin subunit 3 (gamma) (Cct3), mRNA mm
MU19KSUBC:TC39768_S_AT Chaperonin subunit 3 (gamma) (Cct3), mRNA mm
RG-U34A:RC_AA799545_AT chaperonin subunit 3 (gamma) rn
RAE230A:1371403_AT chaperonin subunit 3 (gamma) rn
RAT230_2:1371403_AT chaperonin subunit 3 (gamma) rn
RAE230B:1392091_AT Chaperonin subunit 3 (gamma) rn
RAT230_2:1392091_AT Chaperonin subunit 3 (gamma) rn
RG-U34B:RC_AI043891_AT Chaperonin subunit 3 (gamma) rn
YEAST_2:1773282_AT Subunit of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo Sc
Protein Similarities
Method ID Description E-Value
blast AAF35963 Hypothetical protein F54A3.3 [Caenorhabditis elegans] ref|NP_494218.2| F54A3.3 [Caenorhabditis elegans] 0.0
blast CAE73870 Hypothetical protein CBG21460 [Caenorhabditis briggsae] 0.0
blast XP_392814 PREDICTED: similar to T-complex protein 1, gamma subunit (TCP-1-gamma) (CCT-gamma) [Apis mellifera] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR002423 EMBL-EBI Chaperonin Cpn60/TCP-1 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175916_AT
gaacgtaatctccaagactctcttcacgtcgtccgtaacattatgatcaatccaaagctt
gtaccaggtggcggagctcttgaaatggctttgagccgtgaaattgaacaacaaggagcc
aaaatggacggagttaagaaatggccgtataaggcgattggattggctcttgaagtgatt
ccacggacattaattcagaattgtggaggttctacaattcgaaaaatgaccgaattgcgt
gcgattcacgctcaaaacgctgaaaattggacttttggagtcgacggaacctcgggagac
ttggttgacatgaacaaactggaaatctgggacccactggccgtccgtattcaagttctc
aaaacagccatcgaaacatcggttatgcttctccgtatcgacgata

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAACGTAATCTCCAAGACTCTCTTC 666 347 1369 Antisense
TCTCTTCACGTCGTCCGTAACATTA 293 613 1387 Antisense
GATCAATCCAAAGCTTGTACCAGGT 625 419 1413 Antisense
GGCTCTTGAAGTGATTCCACGGACA 403 537 1533 Antisense
AATGACCGAATTGCGTGCGATTCAC 473 165 1593 Antisense
GTGCGATTCACGCTCAAAACGCTGA 336 479 1607 Antisense
GACTTTTGGAGTCGACGGAACCTCG 624 367 1638 Antisense
GGAACCTCGGGAGACTTGGTTGACA 349 527 1654 Antisense
CTGGAAATCTGGGACCCACTGGCCG 446 241 1687 Antisense
GCCATCGAAACATCGGTTATGCTTC 2 279 1735 Antisense
GTTATGCTTCTCCGTATCGACGATA 614 445 1750 Antisense
Sequence Source Affymetrix Proprietary Database