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[175914_at]

Description F12E12.F /REP_DB=WormBase Gene ID /WP=CE20675 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 93      4grp_total > 26      6grp_total_sub > Hat      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 94
1 2 3 4 range
A 30.7
(-1.8)
A 		22.5
(-3)
A 		65
(3)
A 		57.8
(2)
A 		43
B 29.1
(-3)
A 		91.5
(3)
A 		44.3
(-1.5)
A 		80
(1.9)
A 		62
C 58.2
(-0.5)
A 		75.7
(0.7)
A 		18.7
(-3)
A 		112.2
(3)
A 		94
avr 39.3
(-3) 		63.2
(0.3) 		42.7
(-2.5) 		83.3
(3) 		44

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 88
1 2 3 4 5 6 range
A 35.1
(-1.8)
A 		19.9
(-2.9)
A 		58.9
(-0.2)
A 		18.6
(-3)
A 		103.7
(3)
A 		48.8
(-0.9)
A 		85
B 60.9
(0.9)
A 		66
(1.6)
A 		31
(-2.8)
A 		32.2
(-2.7)
A 		77.4
(3)
A 		29.4
(-3)
A 		48
C 16.9
(-2.8)
A 		46.8
(0.9)
A 		15.5
(-3)
A 		15.7
(-3)
A 		24.7
(-1.8)
A 		63.1
(3)
A 		48
avr 37.6
(-1) 		44.2
(-0.2) 		35.1
(-1.3) 		22.2
(-3) 		68.6
(3) 		47.1
(0.2) 		46
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 18
1 2 3 4 range
A 11.2
(0.1)
A 		4.9
(-3)
A 		17
(3)
A 		8.5
(-1.2)
A 		12
B 9.3
(-3)
A 		22.6
(3)
A 		10.3
(-2.5)
A 		17.1
(0.5)
A 		13
C 19.3
(2.1)
A 		21.7
(3)
A 		6.5
(-3)
A 		18.9
(1.9)
A 		15
avr 13.3
(-0.6) 		16.4
(3) 		11.3
(-3) 		14.8
(1.1) 		5

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 28
1 2 3 4 5 6 range
A 6.5
(-2.6)
A 		7.1
(-2.5)
A 		12.8
(-1.2)
A 		4.7
(-3)
A 		31.3
(3)
A 		11.9
(-1.4)
A 		27
B 21.2
(0.4)
A 		22
(0.6)
A 		7.5
(-3)
A 		8.5
(-2.8)
A 		31.5
(3)
A 		11
(-2.1)
A 		24
C 6.3
(-2)
A 		14.3
(0.7)
A 		3.9
(-2.8)
A 		3.2
(-3)
A 		8
(-1.4)
A 		21.1
(3)
A 		18
avr 11.3
(-1.1) 		14.5
(-0) 		8.1
(-2.1) 		5.5
(-3) 		23.6
(3) 		14.7
(0) 		18
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175914_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18880
Sequence Type Exemplar sequence
Representative Public ID F12E12.F NCBI
Target Description F12E12.F /REP_DB=WormBase Gene ID /WP=CE20675 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Genome Target Overlap based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade B annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062236
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
GENEFINDER00000025188 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3737328:3738457:1 None
F12E12.3 ENSEMBL cdna:known chromosome:CEL140:II:3737328:3738276:1 gene:F12E12.3 A 
SNAP00000025170 ENSEMBL cdna:SNAP chromosome:CEL140:II:3737328:3738046:1 None
NM_062236 NCBI Caenorhabditis elegans F12E12.3 (F12E12.3) mRNA, complete cds. A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3732018-3737520(+) 94.18 100.0
Public Domain and Genome References
Gene Title
Ensembl F12E12.3
Entrez Gene 184387 Entrez gene
SwissProt Q9BLC1 EMBL-EBI
WormBase CE26806 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18927 Hypothetical protein F12E12.3 [Caenorhabditis elegans] ref|NP_494637.1| F12E12.3 [Caenorhabditis elegans] 1.0E-111
blast AAK18927 Hypothetical protein F12E12.3 [Caenorhabditis elegans] ref|NP_494637.1| F12E12.3 [Caenorhabditis elegans] 1.0E-103
blast AAK18927 Hypothetical protein F12E12.3 [Caenorhabditis elegans] ref|NP_494637.1| F12E12.3 [Caenorhabditis elegans] 2.0E-53
blast AAB52884 Hypothetical protein T23C6.3 [Caenorhabditis elegans] ref|NP_510776.1| T23C6.3 [Caenorhabditis elegans] 2.0E-12
blast AAB52884 Hypothetical protein T23C6.3 [Caenorhabditis elegans] ref|NP_510776.1| T23C6.3 [Caenorhabditis elegans] 6.0E-10
blast AAA82378 Hypothetical protein K09E3.7 [Caenorhabditis elegans] ref|NP_510775.1| K09E3.7 [Caenorhabditis elegans] 2.0E-9
blast AAB52884 Hypothetical protein T23C6.3 [Caenorhabditis elegans] ref|NP_510776.1| T23C6.3 [Caenorhabditis elegans] 3.0E-9
Sequence
Target Sequence 
>C. ELEGANS:175914_AT
tttgagattcaagtcctcgttctgcttggcttcctgagctctcttgagctttcgattcag
ccttctcatttcggatctcatctcttccttgtgcttcatgcatttgtccagaagcaagga
atgatggagctccgcttgtgtctggattatgctcatcgtcttactatggatgaactcctt
catttgctctcggagctttcgcctatttttaatgtaatgtatgcttgcctgcctctttgc
cttgccacaggcgtacctgccacttacaataagacacaggagtatgtgccctctttgtgc
ctgagtttacgtcaaaatgcagagagaattataacttgcgatcaaaagcacgatgcctgc
agctccaaactacagaattatggtaagcagctcctacctatcaaaatttccaactttaaa
tctttccagacccttgccgacttgaagaaagccactcaaaagcttgatatcaccattttc
gaccgcaataac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTTGAGATTCAAGTCCTCGTTCTGC 183 667 997 Antisense
GCTCTCTTGAGCTTTCGATTCAGCC 235 301 1034 Antisense
TCGATTCAGCCTTCTCATTTCGGAT 595 603 1048 Antisense
AGCTCCGCTTGTGTCTGGATTATGC 246 81 1124 Antisense
GGATTATGCTCATCGTCTTACTATG 662 509 1140 Antisense
CTCGGAGCTTTCGCCTATTTTTAAT 388 33 1185 Antisense
ATGTAATGTATGCTTGCCTGCCTCT 272 47 1208 Antisense
ACAGGAGTATGTGCCCTCTTTGTGC 572 105 1272 Antisense
ATGCCTGCAGCTCCAAACTACAGAA 342 41 1349 Antisense
GCAGCTCCTACCTATCAAAATTTCC 656 311 1383 Antisense
TATCACCATTTTCGACCGCAATAAC 407 657 1464 Antisense
Sequence Source Affymetrix Proprietary Database