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[175905_at]

Description Y67D8A.F /REP_DB=WormBase Gene ID /WP=CE22768 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_high1_10_10 > 07      4grp_high_8_8 > 35      6grp_total > 06      4grp_total > 53      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 243
1 2 3 4 range
A 468.7
(3)
P 		435
(2.2)
P 		306
(-1)
P 		225.3
(-3)
A 		243
B 393.1
(3)
P 		365.5
(1.3)
P 		297.5
(-3)
P 		297.3
(-3)
P 		96
C 444.7
(3)
P 		405.7
(1.3)
P 		368.2
(-0.3)
P 		307.2
(-3)
A 		138
avr 435.5
(3) 		402.1
(1.7) 		323.9
(-1.2) 		276.6
(-3) 		159

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 390
1 2 3 4 5 6 range
A 618.3
(3)
P 		468.2
(0)
P 		315.6
(-3)
P 		386.2
(-1.6)
P 		410.1
(-1.1)
P 		346.7
(-2.4)
P 		303
B 517.1
(3)
P 		489.7
(2.4)
P 		244.3
(-3)
P 		411
(0.7)
P 		279.2
(-2.2)
P 		382.8
(0)
P 		273
C 517.7
(2.5)
P 		415.8
(0.6)
P 		544.2
(3)
P 		306.3
(-1.5)
A 		388.1
(0)
P 		227.9
(-3)
P 		316
avr 551
(3) 		457.9
(0.6) 		368
(-1.7) 		367.8
(-1.7) 		359.1
(-2) 		319.1
(-3) 		232
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 138
1 2 3 4 range
A 170.6
(3)
P 		94.4
(-0.3)
P 		80
(-1)
P 		33
(-3)
A 		138
B 125.3
(3)
P 		90.3
(-0.4)
P 		68.9
(-2.5)
P 		63.4
(-3)
P 		62
C 147.2
(3)
P 		116.6
(1.1)
P 		128.3
(1.8)
P 		51.8
(-3)
A 		95
avr 147.7
(3) 		100.4
(0.1) 		92.4
(-0.4) 		49.4
(-3) 		98

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 133
1 2 3 4 5 6 range
A 114
(-0.2)
P 		166.5
(3)
P 		68.7
(-3)
P 		97.9
(-1.2)
P 		123.8
(0.4)
P 		84.4
(-2)
P 		98
B 179.9
(3)
P 		163
(2.2)
P 		59.3
(-3)
P 		108.9
(-0.5)
P 		113.4
(-0.3)
P 		142.7
(1.1)
P 		121
C 191.9
(3)
P 		126.9
(0)
P 		138.5
(0.5)
P 		61.6
(-3)
A 		125.6
(-0.1)
P 		76.3
(-2.3)
P 		130
avr 161.9
(3) 		152.1
(2.2) 		88.8
(-3) 		89.5
(-2.9) 		120.9
(-0.4) 		101.1
(-2) 		73
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175905_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19219
Sequence Type Exemplar sequence
Representative Public ID Y67D8A.F NCBI
Target Description Y67D8A.F /REP_DB=WormBase Gene ID /WP=CE22768 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067903(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067903 NCBI Caenorhabditis elegans Y67D8A.1 (Y67D8A.1) mRNA, complete cds. 10/11 A 
GENEFINDER00000035506 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:3222440:3248145:1 10/11 None
Y67D8A.1.2 ENSEMBL cdna:known chromosome:CEL140:IV:3225606:3252571:1 gene:Y67D8A.1 10/11 A 
Y67D8A.1.1 ENSEMBL cdna:known chromosome:CEL140:IV:3225606:3252575:1 gene:Y67D8A.1 10/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000035501 8/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:3239916-3248151(+) 96.43 100.0
Public Domain and Genome References
Gene Title
Ensembl Y67D8A.1
Entrez Gene 177094 Entrez gene
SwissProt Q95XU2 EMBL-EBI
WormBase CE31660 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1598099_AT similar to phosphorylase kinase beta cfa
CANINE_2:CFA.10856.1.S1_AT similar to phosphorylase kinase beta cfa
CANINE_2:CFAAFFX.15996.1.S1_AT similar to phosphorylase kinase beta cfa
DROSOPHILA_2:1625204_A_AT dm
DROSGENOME1:152396_AT dm
CHICKEN:GGAAFFX.2485.1.S1_S_AT phosphorylase kinase, beta gga
CHICKEN:GGAAFFX.2485.2.S1_AT phosphorylase kinase, beta gga
CHICKEN:GGAAFFX.2485.3.S1_S_AT phosphorylase kinase, beta gga
HG-U133_PLUS_2:202739_S_AT phosphorylase kinase, beta hs
HG-U133A:202739_S_AT phosphorylase kinase, beta hs
HG-FOCUS:202739_S_AT phosphorylase kinase, beta hs
HG-U133A_2:202739_S_AT phosphorylase kinase, beta hs
HUGENEFL:X84908_AT phosphorylase kinase, beta hs
U133_X3P:G4505782_3P_A_AT phosphorylase kinase, beta hs
U133_X3P:HS.78060.0.A1_3P_A_AT phosphorylase kinase, beta hs
U133_X3P:HS.269887.0.A1_3P_X_AT phosphorylase kinase, beta hs
U133_X3P:HS.269887.0.A1_3P_AT phosphorylase kinase, beta hs
HG-U133A_2:202738_S_AT phosphorylase kinase, beta hs
HG-U133_PLUS_2:202738_S_AT phosphorylase kinase, beta hs
HG-U133A:202738_S_AT phosphorylase kinase, beta hs
HG-U95AV2:37392_AT phosphorylase kinase, beta hs
HG-U133_PLUS_2:242248_AT Phosphorylase kinase, beta hs
HG-U133B:242248_AT Phosphorylase kinase, beta hs
HG-U95D:85900_R_AT Phosphorylase kinase, beta hs
HG-U133_PLUS_2:238601_AT Phosphorylase kinase, beta hs
HG-U133B:238601_AT Phosphorylase kinase, beta hs
HG-U95E:83040_AT Phosphorylase kinase, beta hs
U133_X3P:HS.148154.0.A1_3P_AT Phosphorylase kinase, beta hs
MU11KSUBB:MSA.40053.0_S_AT phosphorylase kinase beta mm
MU11KSUBB:MSA.24732.0_S_AT phosphorylase kinase beta mm
MG-U74BV2:109086_AT phosphorylase kinase beta mm
MOE430A:1434511_AT phosphorylase kinase beta mm
MOUSE430A_2:1434511_AT phosphorylase kinase beta mm
MOUSE430_2:1434511_AT phosphorylase kinase beta mm
MG-U74AV2:96722_AT phosphorylase kinase beta mm
MG-U74BV2:113682_AT Phosphorylase kinase beta (Phkb), mRNA mm
MOE430B:1442053_AT Phosphorylase kinase beta (Phkb), mRNA mm
MOUSE430_2:1442053_AT Phosphorylase kinase beta (Phkb), mRNA mm
MU19KSUBC:TC35952_AT Phosphorylase kinase beta (Phkb), mRNA mm
MU19KSUBC:TC35953_AT Phosphorylase kinase beta (Phkb), mRNA mm
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
5977 glycogen metabolism inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3824 catalytic activity inferred from electronic annotation QuickGO AmiGO
5516 calmodulin binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK68543 Hypothetical protein Y67D8A.1 [Caenorhabditis elegans] ref|NP_500304.2| Y67D8A.1 [Caenorhabditis elegans] 0.0
blast CAE59898 Hypothetical protein CBG03382 [Caenorhabditis briggsae] 0.0
blast AAF52613 CG8475-PA, isoform A [Drosophila melanogaster] ref|NP_609189.2| CG8475-PA, isoform A [Drosophila melanogaster] sp|Q9VLS1|KPBB_DROME Probable phosphorylase b kinase beta regulatory chain (Phosphorylase kinase beta subunit) 0.0
blast AAA31448 phosphorylase kinase beta-subunit 1.0E-163
Protein Domains
Database ID Description E-Value
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.2E-31
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 6.3E-37
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.0E-126
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 1.2E-31
Pfam IPR008734 EMBL-EBI Phosphorylase kinase alphabeta 2.0E-46
Sequence
Target Sequence 
>C. ELEGANS:175905_AT
ttggcatttgttatcctctctgcaaacccgccggctcaacggagcactcaaccggatgcc
ggtcaacttttacgatcgagtctggacgatactggaaagatcaacgcatggaatcatcat
tgccgatcagttcctaccacaacagccgacactcagcgatatgacaagattcgagttgac
gttctcttataagattgaggcgatgcttagcagaatatcacatcctgagtataggcagtt
gctcgttgagaatagggccgaaaaattccgattttcttcaaaaattgctgaaaattgccg
attttttccgcagttgctgaaaatgctcctctcgatcatcgccacaattctggaacgtaa
cccggaaatcgcgttcactcattcccgaatcgattgtgatagtttgatcaaaaagggatt
cgccctctttgctgcagaagagggcatcaaggatctcaacgatttgacaccgttctatca
actagaaggacaggctctctccacttcaacggccacgtttttgacaaaggtaatcccact
gactgcaactttttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TTGGCATTTGTTATCCTCTCTGCAA 205 709 687 Antisense
TCAACGGAGCACTCAACCGGATGCC 490 631 722 Antisense
GGATGCCGGTCAACTTTTACGATCG 557 513 740 Antisense
GAATCATCATTGCCGATCAGTTCCT 514 327 797 Antisense
ACAACAGCCGACACTCAGCGATATG 196 113 825 Antisense
CAGTTGCTGAAAATGCTCCTCTCGA 199 207 997 Antisense
GTTCACTCATTCCCGAATCGATTGT 365 445 1059 Antisense
AAAAAGGGATTCGCCCTCTTTGCTG 688 129 1096 Antisense
AACTAGAAGGACAGGCTCTCTCCAC 585 139 1166 Antisense
TCCACTTCAACGGCCACGTTTTTGA 284 589 1186 Antisense
TAATCCCACTGACTGCAACTTTTTT 117 637 1217 Antisense
Sequence Source Affymetrix Proprietary Database