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[175895_at]

Description ZK353.1 /REP_DB=WormBase Gene ID /WP=CE25684 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 63      6grp_high1_10_10 > 36      4grp_high_8_8 > 18      6grp_highest_nonorm > 81      6grp_total > 36      4grp_total > 29      6grp_highest_sub > Cu      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 164
1 2 3 4 range
A 253.2
(3)
P 		203.4
(0.7)
P 		120.7
(-3)
P 		225.1
(1.7)
M 		133
B 269.3
(3)
P 		199.1
(-0.7)
P 		154.2
(-3)
P 		235.5
(1.2)
P 		115
C 284.6
(3)
P 		200.7
(-2)
P 		183.4
(-3)
P 		266.6
(1.9)
P 		101
avr 269
(3) 		201.1
(-0.5) 		152.8
(-3) 		242.4
(1.6) 		116

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 234
1 2 3 4 5 6 range
A 379.6
(3)
P 		229.6
(-0.9)
P 		248.4
(-0.4)
P 		150.4
(-3)
P 		210.5
(-1.4)
P 		237
(-0.7)
P 		229
B 259.5
(1)
P 		296.1
(3)
P 		241.8
(0.1)
P 		234.9
(-0.3)
P 		183.8
(-3)
P 		290.9
(2.7)
P 		112
C 270.9
(1.5)
P 		314
(3)
P 		259.6
(1.1)
P 		208.1
(-0.8)
P 		146
(-3)
P 		274.8
(1.6)
P 		168
avr 303.3
(3) 		279.9
(1.9) 		249.9
(0.4) 		197.8
(-2.1) 		180.1
(-3) 		267.6
(1.3) 		123
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 63
1 2 3 4 range
A 92.2
(3)
P 		44.2
(-1.7)
P 		31.5
(-3)
P 		33
(-2.9)
M 		61
B 85.9
(3)
P 		49.2
(-1.4)
P 		35.7
(-3)
P 		50.2
(-1.3)
P 		50
C 94.2
(3)
P 		57.7
(-1.5)
P 		63.9
(-0.7)
P 		45
(-3)
P 		49
avr 90.8
(3) 		50.4
(-2) 		43.7
(-2.9) 		42.7
(-3) 		48

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 70
1 2 3 4 5 6 range
A 70
(1.4)
P 		81.7
(3)
P 		54.1
(-0.8)
P 		38.2
(-3)
P 		63.5
(0.5)
P 		57.7
(-0.3)
P 		44
B 90.3
(0.8)
P 		98.6
(1.8)
P 		58.7
(-3)
P 		62.2
(-2.6)
P 		74.7
(-1.1)
P 		108.5
(3)
P 		50
C 100.4
(3)
P 		95.8
(2.5)
P 		66.1
(-0.5)
P 		41.9
(-3)
P 		47.2
(-2.5)
P 		92
(2.1)
P 		59
avr 86.9
(2.3) 		92
(3) 		59.6
(-1.4) 		47.4
(-3) 		61.8
(-1.1) 		86.1
(2.2) 		45
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175895_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.7274
Sequence Type Exemplar sequence
Representative Public ID ZK353.1 NCBI
Target Description ZK353.1 /REP_DB=WormBase Gene ID /WP=CE25684 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066457(10), NM_066456(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066457 NCBI Caenorhabditis elegans ZK353.1a (ZK353.1) mRNA, complete cds. 10/11 None
NM_066456 NCBI Caenorhabditis elegans ZK353.1b (ZK353.1) mRNA, complete cds. 10/11 None
SNAP00000044098 ENSEMBL cdna:SNAP chromosome:CEL140:III:8405923:8408755:1 10/11 None
GENEFINDER00000044103 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8405923:8408755:1 10/11 None
ZK353.1a ENSEMBL cdna:known chromosome:CEL140:III:8405789:8409317:1 gene:ZK353.1 10/11 A 
ZK353.1b ENSEMBL cdna:known chromosome:CEL140:III:8405821:8408755:1 gene:ZK353.1 10/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8405918-8408940(+) 100.0 100.0
Public Domain and Genome References
Ensembl ZK353.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAP68920 Hypothetical protein ZK353.1a [Caenorhabditis elegans] ref|NP_498858.2| ZK353.1a [Caenorhabditis elegans] 0.0
blast AAP68921 Hypothetical protein ZK353.1b [Caenorhabditis elegans] ref|NP_498857.2| ZK353.1b [Caenorhabditis elegans] sp|P34624|YOJ1_CAEEL Hypothetical protein ZK353.1 in chromosome III 0.0
blast CAE71262 Hypothetical protein CBG18144 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006671 EMBL-EBI Cyclin, N-terminal domain 4.6E-8
Pfam IPR006671 EMBL-EBI Cyclin, N-terminal domain 4.6E-8
Pfam IPR006671 EMBL-EBI Cyclin, N-terminal domain 4.6E-8
Sequence
Target Sequence 
>C. ELEGANS:175895_AT
gtttagttctgcgcagttgaccgccgaatgtgctattattactttggtgtacatcgaacg
attgcttaattacgcagaaatggatttgtgtccttcaaattggcgtcgggttgtactcgg
ttcaatcatgttggcatcaaaagtatgggatgatcaagctgtttggaatgttgattattg
ccaaattcttcgagatacaaatgttgacgatatgaacgaattagaacggcggtttttgga
atgtctcgactttaatattgaagttccgtcgtcggtgtatgccaagtattatttcgatct
tcgaacattggcacttgcaaatgatttacaattaccaattcaaccgttgtataaggagag
agcgcaaagattagaggctttgagccgtgtcttcgaggataagattcaatcatcatcgct
tccaaagcgtgcccggtcagctgaacatcttgtctttgagcatccggctgttctctcttt
caatggatttg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTTTAGTTCTGCGCAGTTGACCGCC 215 449 600 Antisense
GTTGACCGCCGAATGTGCTATTATT 224 439 615 Antisense
TTGTGTCCTTCAAATTGGCGTCGGG 551 707 685 Antisense
TCGGGTTGTACTCGGTTCAATCATG 267 605 705 Antisense
GGCGGTTTTTGGAATGTCTCGACTT 487 541 827 Antisense
TTCCGTCGTCGGTGTATGCCAAGTA 165 691 863 Antisense
GATCTTCGAACATTGGCACTTGCAA 64 419 895 Antisense
AGAGGCTTTGAGCCGTGTCTTCGAG 16 69 972 Antisense
GATTCAATCATCATCGCTTCCAAAG 343 431 1002 Antisense
TGAACATCTTGTCTTTGAGCATCCG 506 573 1041 Antisense
GCTGTTCTCTCTTTCAATGGATTTG 331 295 1066 Antisense
Sequence Source Affymetrix Proprietary Database