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[175892_at]

Description ZK616.F /REP_DB=WormBase Gene ID /WP=CE26348 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 88      4grp_total > 0      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 9.9
(-1.1)
A 		7.5
(-3)
A 		12.7
(1.2)
A 		14.9
(3)
A 		7
B 11.8
(-1.6)
A 		6.9
(-3)
A 		15.1
(-0.7)
A 		28
(3)
A 		21
C 7.3
(0.2)
A 		6.5
(-1.3)
A 		8.8
(3)
A 		5.6
(-3)
A 		3
avr 9.7
(-1.2) 		7
(-3) 		12.2
(0.4) 		16.2
(3) 		9

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 276
1 2 3 4 5 6 range
A 4.6
(-3)
A 		6.4
(-3)
A 		38.7
(-2.2)
A 		27.6
(-2.4)
A 		115.2
(-0.3)
A 		247.9
(3)
P 		243
B 8
(-2.9)
A 		8.5
(-2.9)
A 		5.3
(-3)
A 		16.3
(-2.7)
A 		110.2
(-0.2)
P 		230.6
(3)
P 		225
C 5.4
(-3)
A 		5
(-3)
A 		9.6
(-2.9)
A 		9.3
(-2.9)
A 		162
(0.4)
P 		280.3
(3)
P 		275
avr 6
(-3) 		6.6
(-3) 		17.9
(-2.7) 		17.7
(-2.7) 		129.1
(-0) 		252.9
(3) 		247
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 5
1 2 3 4 range
A 3.6
(3)
A 		1.6
(-3)
A 		3.3
(2.1)
A 		2.2
(-1.2)
A 		2
B 3.8
(-0.1)
A 		1.7
(-3)
A 		3.5
(-0.5)
A 		6
(3)
A 		4
C 2.4
(1.1)
A 		1.9
(-0.3)
A 		3.1
(3)
A 		0.9
(-3)
A 		2
avr 3.3
(3) 		1.7
(-3) 		3.3
(3) 		3
(1.9) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 93
1 2 3 4 5 6 range
A 0.9
(-3)
A 		2.3
(-2.9)
A 		8.4
(-2.2)
A 		7
(-2.4)
A 		34.8
(0.4)
A 		60.4
(3)
P 		60
B 2.8
(-2.9)
A 		2.8
(-2.9)
A 		1.3
(-3)
A 		4.3
(-2.8)
A 		44.8
(0.1)
P 		86
(3)
P 		85
C 2
(-3)
A 		1.5
(-3)
A 		2.4
(-2.9)
A 		1.9
(-3)
A 		52.4
(0.3)
P 		93.8
(3)
P 		92
avr 1.9
(-3) 		2.2
(-3) 		4
(-2.8) 		4.4
(-2.8) 		44
(0.2) 		80.1
(3) 		78
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175892_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19286
Sequence Type Exemplar sequence
Representative Public ID ZK616.F NCBI
Target Description ZK616.F /REP_DB=WormBase Gene ID /WP=CE26348 /CHR=4 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_068399(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_068399 NCBI Caenorhabditis elegans ZK616.5 (ZK616.5) mRNA, complete cds. 9/11 A 
SNAP00000016312 ENSEMBL cdna:SNAP chromosome:CEL140:IV:5392161:5393031:1 9/11 None
GENEFINDER00000016325 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:5392161:5393031:1 9/11 None
ZK616.5 ENSEMBL cdna:known chromosome:CEL140:IV:5392161:5393031:1 gene:ZK616.5 9/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
NM_068579 1/11 Cross Hyb Matching Probes A C 
SNAP00000032057 1/11 Cross Hyb Matching Probes None
GENEFINDER00000032099 1/11 Cross Hyb Matching Probes None
Y73B6BL.23 1/11 Cross Hyb Matching Probes A C 
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:5392166-5393037(+) 100.0 100.0
chrIV:6418192-6419068(-) 94.68 94.68
Public Domain and Genome References
Gene Title
Ensembl ZK616.5
Entrez Gene 177323 Entrez gene
SwissProt Q965Z2 EMBL-EBI
WormBase CE27558 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68624 Hypothetical protein ZK616.5 [Caenorhabditis elegans] ref|NP_500800.1| ZK616.5 [Caenorhabditis elegans] 1.0E-104
blast AAK68593 Hypothetical protein Y73B6BL.23 [Caenorhabditis elegans] ref|NP_500980.1| Y73B6BL.23 [Caenorhabditis elegans] 2.0E-93
Sequence
Target Sequence 
>C. ELEGANS:175892_AT
agagcactccccgaggtatttgaataaaaaatgtcattttttcgagtactttcttttcag
caaaggcgcatacagggacaaaatctggagcggtccagtcagtgaggtcgccaggcaaca
aacggtgtacgactgggtgactacaggtgcttattcccttggatcgccagactcgtggcc
atcttatccggacgaaatcatactgatgaacgagtcccgaaatgctcagacatcccgcat
gtcgcgtcggtcggctactcccggtccaagattgtcacaagatagaaggacaagaactac
agcacccgccgttgcgccgattccgtttcagagggcacagcctcgagtagaagtggagag
acaaagaatcgacgagcctgtcgcacaggacctgattatcgatgatgggaacggtaccaa
caattcgaacgattcgagtatttgtggctttgtggaggaatccgaggaactcgtatatcc
acagtgggtt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAGCACTCCCCGAGGTATTTGAAT 435 69 72 Antisense
TTTTCGAGTACTTTCTTTTCAGCAA 10 677 110 Antisense
CTCCCGGTCCAAGATTGTCACAAGA 678 229 329 Antisense
GAAGGACAAGAACTACAGCACCCGC 632 333 356 Antisense
TGCGCCGATTCCGTTTCAGAGGGCA 476 583 384 Antisense
AGGGCACAGCCTCGAGTAGAAGTGG 121 59 403 Antisense
AAGAATCGACGAGCCTGTCGCACAG 701 151 435 Antisense
GCCTGTCGCACAGGACCTGATTATC 365 283 447 Antisense
GATGGGAACGGTACCAACAATTCGA 701 405 475 Antisense
GAACGATTCGAGTATTTGTGGCTTT 68 349 498 Antisense
GGAACTCGTATATCCACAGTGGGTT 461 529 537 Antisense
Sequence Source Affymetrix Proprietary Database