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[175889_at]

Description F44A2.1 /REP_DB=WormBase Gene ID /WP=CE04568 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_high1_10_10 > 88      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_total > 87      4grp_total > 39      6grp_cgc > 59      6grp_high1_sub > Da      6grp_cgc_sub > Da      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 177
1 2 3 4 range
A 452.7
(3)
P 		412.4
(0.3)
P 		408.7
(0.1)
P 		362.2
(-3)
P 		91
B 482.4
(2.6)
P 		394.7
(-1.6)
P 		364.2
(-3)
P 		491
(3)
P 		127
C 539.6
(3)
P 		378.2
(-3)
P 		421.7
(-1.4)
P 		429.1
(-1.1)
P 		161
avr 491.6
(3) 		395.1
(-3) 		398.2
(-2.8) 		427.4
(-1) 		97

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 650
1 2 3 4 5 6 range
A 251.3
(-3)
A 		326.2
(-2.2)
P 		250
(-3)
P 		324.6
(-2.2)
P 		478.8
(-0.6)
P 		812.1
(3)
P 		562
B 224.8
(-2.7)
A 		197.5
(-3)
P 		298.7
(-1.9)
P 		326
(-1.6)
P 		453.1
(-0.1)
P 		734.1
(3)
P 		537
C 285.6
(-2.5)
P 		238.4
(-3)
A 		346.2
(-1.9)
P 		299
(-2.4)
P 		569.5
(0.3)
P 		847.6
(3)
P 		609
avr 253.9
(-3) 		254
(-3) 		298.3
(-2.5) 		316.5
(-2.3) 		500.5
(-0.3) 		797.9
(3) 		544
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 126
1 2 3 4 range
A 164.8
(3)
P 		89.5
(-1)
P 		106.8
(-0.1)
P 		53
(-3)
P 		112
B 153.8
(3)
P 		97.5
(-1.9)
P 		84.4
(-3)
P 		104.6
(-1.3)
P 		69
C 178.6
(3)
P 		108.7
(-0.9)
P 		147
(1.2)
P 		72.4
(-3)
P 		106
avr 165.7
(3) 		98.6
(-1.5) 		112.7
(-0.6) 		76.7
(-3) 		89

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 237
1 2 3 4 5 6 range
A 46.3
(-3)
A 		116
(-0.2)
P 		54.5
(-2.7)
P 		82.3
(-1.6)
P 		144.5
(0.9)
P 		197.7
(3)
P 		151
B 78.2
(-2.6)
A 		65.7
(-3)
P 		72.5
(-2.8)
P 		86.3
(-2.4)
P 		184.1
(0.4)
P 		273.8
(3)
P 		208
C 105.8
(-1.8)
P 		72.7
(-2.7)
A 		88.1
(-2.2)
P 		60.1
(-3)
P 		184.3
(0.3)
P 		283.7
(3)
P 		224
avr 76.8
(-2.8) 		84.8
(-2.6) 		71.7
(-3) 		76.2
(-2.9) 		171
(0.3) 		251.7
(3) 		180
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175889_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11736
Sequence Type Exemplar sequence
Representative Public ID F44A2.1 NCBI
Target Description F44A2.1 /REP_DB=WormBase Gene ID /WP=CE04568 /CHR=5 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_073008(11), NM_073009(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_073008 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-153) (tag-153) mRNA, complete cds. 11/11 None
NM_073009 NCBI Caenorhabditis elegans Temporarily Assigned Gene name family member (tag-153) (tag-153) mRNA, complete cds. 11/11 None
SNAP00000038855 ENSEMBL cdna:SNAP chromosome:CEL140:V:9327340:9329308:1 11/11 None
GENEFINDER00000038864 ENSEMBL cdna:GeneFinder chromosome:CEL140:V:9325968:9329308:1 11/11 None
F44A2.1b ENSEMBL cdna:known chromosome:CEL140:V:9325795:9329793:1 gene:F44A2.1 11/11 A 
F44A2.1a ENSEMBL cdna:known chromosome:CEL140:V:9326940:9329308:1 gene:F44A2.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:9327339-9329308(+) 100.0 100.0
Public Domain and Genome References
Ensembl F44A2.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA83450 Temporarily assigned gene name protein 153, isoform b [Caenorhabditis elegans] ref|NP_505409.1| Temporarily Assigned Gene name family member (tag-153) [Caenorhabditis elegans] 0.0
blast AAA83449 Temporarily assigned gene name protein 153, isoform a [Caenorhabditis elegans] ref|NP_505410.1| Temporarily Assigned Gene name family member (tag-153) [Caenorhabditis elegans] 0.0
blast CAE72152 Hypothetical protein CBG19250 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR007282 EMBL-EBI NOT2/NOT3/NOT5 6.9E-72
Pfam IPR007282 EMBL-EBI NOT2/NOT3/NOT5 6.9E-72
Sequence
Target Sequence 
>C. ELEGANS:175889_AT
atcacagctccacaaaacattttcactgtgccagcaagcaatcaggaagatgtgatgatt
ggaatattcgagatttatgatgccgaaaaggctcgcatttgttccgccgaattgtgcctt
cgtcgttctgacttcgaggttccagcatgggaacagaaagttccggatgcctatcaaaaa
atggtgtcggcaatgattccaaaggaaatgctgtggggaccacgtgaagatcgcaagcaa
tatggattgatgcaaggaataactggacttcttgctccaggtcatggaagaaacaaagta
tccaatttggtcattccaaagcaacaaccatcagagcgtcgtttgcttagcttcgccgag
tctccagatatcacgccaacctttgacaatccatttggcaccaatccgacaggcaacaca
gctgaggatctttaccgccaacagatgtatgcgatgattgttcagaagatgcaaatgcac
cagcaacagcagttccaacagcatcagcaacttcagtcaggaggatccgcttcaaacctg
tcgaagc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATCACAGCTCCACAAAACATTTTCA 682 27 919 Antisense
CCGAAAAGGCTCGCATTTGTTCCGC 208 261 1001 Antisense
CTTCGTCGTTCTGACTTCGAGGTTC 202 219 1036 Antisense
GGGGACCACGTGAAGATCGCAAGCA 166 499 1133 Antisense
ACTGGACTTCTTGCTCCAGGTCATG 117 97 1180 Antisense
GCAACAACCATCAGAGCGTCGTTTG 512 317 1239 Antisense
GATATCACGCCAACCTTTGACAATC 120 427 1285 Antisense
CACCAATCCGACAGGCAACACAGCT 543 201 1317 Antisense
AACACAGCTGAGGATCTTTACCGCC 72 135 1333 Antisense
AGCAACTTCAGTCAGGAGGATCCGC 288 77 1424 Antisense
GGATCCGCTTCAAACCTGTCGAAGC 262 511 1441 Antisense
Sequence Source Affymetrix Proprietary Database