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[175886_at]

Description F54A3.2 /REP_DB=WormBase Gene ID /WP=CE25899 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Cluster 4grp_high_8_8 > 37      6grp_total > 19      4grp_total > 34      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 51.3
(-3)
P 		100.4
(0.4)
P 		88
(-0.4)
P 		137.3
(3)
P 		86
B 112.5
(1.6)
P 		130.3
(3)
P 		111.1
(1.4)
P 		56.5
(-3)
P 		74
C 92.1
(-0)
P 		105.7
(1.3)
A 		62
(-3)
P 		123.1
(3)
A 		61
avr 85.3
(-3) 		112.1
(3) 		87
(-2.6) 		105.6
(1.5) 		27

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 48
1 2 3 4 5 6 range
A 93.9
(3)
P 		70.4
(0.1)
P 		77.4
(0.9)
M 		46
(-3)
A 		78.8
(1.1)
P 		74.1
(0.5)
P 		48
B 85.4
(3)
P 		45.8
(-3)
P 		51
(-2.2)
A 		74.7
(1.4)
M 		65.8
(0)
M 		59.8
(-0.9)
P 		40
C 86.2
(3)
P 		53.9
(-2.9)
P 		73.8
(0.7)
P 		53.6
(-3)
A 		76.5
(1.2)
P 		70.2
(0.1)
P 		33
avr 88.5
(3) 		56.7
(-3) 		67.4
(-1) 		58.1
(-2.7) 		73.7
(0.2) 		68
(-0.9) 		32
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 24
1 2 3 4 range
A 18.7
(-3)
P 		21.8
(1.3)
P 		23
(3)
P 		20.1
(-1)
P 		4
B 35.9
(3)
P 		32.2
(2.1)
P 		25.8
(0.5)
P 		12
(-3)
P 		24
C 30.5
(3)
P 		30.4
(2.9)
A 		21.6
(-2.5)
P 		20.8
(-3)
A 		10
avr 28.4
(3) 		28.1
(2.8) 		23.5
(0.3) 		17.6
(-3) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 21
1 2 3 4 5 6 range
A 17.3
(-0.5)
P 		25
(3)
P 		16.8
(-0.7)
M 		11.7
(-3)
A 		23.8
(2.5)
P 		18
(-0.2)
P 		13
B 29.7
(3)
P 		15.2
(-2)
P 		12.4
(-3)
A 		19.8
(-0.4)
M 		26.8
(2)
M 		22.3
(0.4)
P 		17
C 32
(3)
P 		16.5
(-1.4)
P 		18.8
(-0.7)
P 		10.8
(-3)
A 		24.8
(1)
P 		23.5
(0.6)
P 		21
avr 26.3
(3) 		18.9
(-0.6) 		16
(-2.1) 		14.1
(-3) 		25.1
(2.4) 		21.3
(0.5) 		12
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175886_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.4795
Sequence Type Exemplar sequence
Representative Public ID F54A3.2 NCBI
Target Description F54A3.2 /REP_DB=WormBase Gene ID /WP=CE25899 /SUBMIT=ST.LOUIS /CHR=2 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_061818(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_061818 NCBI Caenorhabditis elegans F54A3.2 (F54A3.2) mRNA, complete cds. 11/11 None
F54A3.2 ENSEMBL cdna:known chromosome:CEL140:II:2244441:2247052:1 gene:F54A3.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:2243654-2247051(+) 81.04 99.64
Public Domain and Genome References
Gene Title
Ensembl F54A3.2
Entrez Gene 186196 Entrez gene
SwissProt Q9N4J7 EMBL-EBI
WormBase CE32675 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAF35964 Hypothetical protein F54A3.2 [Caenorhabditis elegans] ref|NP_494219.2| F54A3.2 [Caenorhabditis elegans] 1.0E-113
blast CAE73869 Hypothetical protein CBG21459 [Caenorhabditis briggsae] 2.0E-59
blast AAH91977 Im:7143385 protein [Danio rerio] 1.0E-9
Sequence
Target Sequence 
>C. ELEGANS:175886_AT
aaaaatccaattctcgctctaaacactcaaattgagcaaaatccgcggctctcgacctcc
acaatgacaatttacggcgaattaatggatgaattgagaaaaatattggatcgggaatcg
aatattgttgtagttacgacagcaattcgtactgtcgaactattggctcggggaatacgg
caacgatttgctggatttattggaatgctcaaaattcgaaaattgcgtaaaatcgggtgt
tttggaggtactgtagtcggtgtagtgccatttctgatcaaacgtgccaaggataagaag
aagaacgtgagagatgcgattttggcggcaatgggtgctgtggctgatacgacaacttcc
gagcgtctccaaaaggatcttatcgattggttcggaattccgtctccagagtcaaaaaag
acacttttacactttctttacgcatatttctgccgccaatgcacaccggatatccaattt
attaagacgatcgctccgttgattgttaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAAAATCCAATTCTCGCTCTAAACA 490 127 238 Antisense
TCTCGACCTCCACAATGACAATTTA 559 611 287 Antisense
GTACTGTCGAACTATTGGCTCGGGG 607 457 386 Antisense
AGTCGGTGTAGTGCCATTTCTGATC 416 61 492 Antisense
TGGCGGCAATGGGTGCTGTGGCTGA 312 545 560 Antisense
TGATACGACAACTTCCGAGCGTCTC 704 563 582 Antisense
TTCGGAATTCCGTCTCCAGAGTCAA 51 695 628 Antisense
GACACTTTTACACTTTCTTTACGCA 571 365 657 Antisense
TCTTTACGCATATTTCTGCCGCCAA 673 615 672 Antisense
CCGCCAATGCACACCGGATATCCAA 430 261 690 Antisense
AGACGATCGCTCCGTTGATTGTTAA 386 69 722 Antisense
Sequence Source Affymetrix Proprietary Database