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[175881_at]

Description F12E12.G /REP_DB=WormBase Gene ID /WP=CE20676 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_total > 30      4grp_total > 18      6grp_total_sub > Hat      6grp_rule_based > S3      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 45
1 2 3 4 range
A 4.1
(-3)
A 		10
(3)
A 		6.3
(-0.8)
A 		9.9
(2.9)
A 		6
B 1.9
(-3)
A 		9
(3)
A 		4.8
(-0.5)
A 		5.5
(0)
A 		7
C 2.4
(-3)
A 		3.1
(-2.9)
A 		2.2
(-3)
A 		46.9
(3)
A 		45
avr 2.8
(-3) 		7.4
(-1.5) 		4.4
(-2.5) 		20.8
(3) 		18

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 10
1 2 3 4 5 6 range
A 6.9
(0.3)
A 		8.4
(1.2)
A 		11.3
(3)
A 		7.4
(0.6)
A 		1.6
(-3)
A 		3.1
(-2.1)
A 		10
B 2.2
(-2.5)
A 		3.7
(-1)
A 		7.7
(3)
A 		1.8
(-2.9)
A 		1.7
(-3)
A 		2.1
(-2.6)
A 		6
C 1.9
(-3)
A 		8.8
(3)
A 		5.8
(0.4)
A 		5.6
(0.2)
A 		1.9
(-3)
A 		3.9
(-1.3)
A 		7
avr 3.7
(-1.2) 		7
(1.8) 		8.3
(3) 		4.9
(-0.1) 		1.7
(-3) 		3
(-1.8) 		7
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 7
1 2 3 4 range
A 1.5
(-2.3)
A 		2.2
(3)
A 		1.6
(-1.5)
A 		1.4
(-3)
A 		1
B 0.6
(-3)
A 		2.2
(3)
A 		1.1
(-1.1)
A 		1.2
(-0.8)
A 		2
C 0.8
(-3)
A 		0.9
(-2.9)
A 		0.8
(-3)
A 		7.9
(3)
A 		7
avr 1
(-3) 		1.8
(-1.1) 		1.2
(-2.5) 		3.5
(3) 		3

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 3
1 2 3 4 5 6 range
A 1.3
(-1.1)
A 		3
(3)
A 		2.5
(1.8)
A 		1.9
(0.4)
A 		0.5
(-3)
A 		0.8
(-2.3)
A 		3
B 0.8
(-1.7)
A 		1.2
(-0)
A 		1.9
(3)
A 		0.5
(-3)
A 		0.7
(-2.1)
A 		0.8
(-1.7)
A 		1
C 0.7
(-2.7)
A 		2.7
(3)
A 		1.5
(-0.4)
A 		1.1
(-1.6)
A 		0.6
(-3)
A 		1.3
(-1)
A 		2
avr 0.9
(-1.9) 		2.3
(3) 		2
(1.9) 		1.2
(-0.9) 		0.6
(-3) 		1
(-1.6) 		2
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175881_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18886
Sequence Type Exemplar sequence
Representative Public ID F12E12.G NCBI
Target Description F12E12.G /REP_DB=WormBase Gene ID /WP=CE20676 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062236(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062236 NCBI Caenorhabditis elegans F12E12.3 (F12E12.3) mRNA, complete cds. 9/11 None
GENEFINDER00000025188 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:3737328:3738457:1 11/11 None
F12E12.3 ENSEMBL cdna:known chromosome:CEL140:II:3737328:3738276:1 gene:F12E12.3 9/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000025170 5/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:3737700-3738275(+) 100.0 100.0
Public Domain and Genome References
Gene Title
Ensembl F12E12.3
Entrez Gene 184387 Entrez gene
SwissProt Q9BLC1 EMBL-EBI
WormBase CE26806 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK18927 Hypothetical protein F12E12.3 [Caenorhabditis elegans] ref|NP_494637.1| F12E12.3 [Caenorhabditis elegans] 1.0E-111
blast AAK18927 Hypothetical protein F12E12.3 [Caenorhabditis elegans] ref|NP_494637.1| F12E12.3 [Caenorhabditis elegans] 1.0E-103
blast AAB52884 Hypothetical protein T23C6.3 [Caenorhabditis elegans] ref|NP_510776.1| T23C6.3 [Caenorhabditis elegans] 2.0E-12
blast AAB52884 Hypothetical protein T23C6.3 [Caenorhabditis elegans] ref|NP_510776.1| T23C6.3 [Caenorhabditis elegans] 6.0E-10
Sequence
Target Sequence 
>C. ELEGANS:175881_AT
gaggcaatcctgcaaaagtctccaggcatcgaggtggatgtgagattgcaatttgaaatc
caagatgatcatcagatctacgaaaacttcaaggacaagcatcaagaagccatgaactac
aagtcttggaagcaccagcttgacattgtaatagcccagcagacgctggataatctcttg
gctacccagggaagaagctcaaagagttgtatatgggaaataattgcaaagcaacagctg
aaaagggactgctacaagaatttggagaaaattgaaactttgattgggaagctggacctg
cttcaggattgtgtggctcagttgaagcatagtatctggagaaacaagctcgaaatctac
agcatcttcttcaagctcttttgtcgaactgcggagcccgccgacactgagctagtcaac
agaatcgtcgagcatttttctaacaactttccgcaaccagagagtcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GAGGCAATCCTGCAAAAGTCTCCAG 513 405 16 Antisense
AAGTCTCCAGGCATCGAGGTGGATG 277 157 31 Antisense
GACATTGTAATAGCCCAGCAGACGC 117 371 157 Antisense
GACGCTGGATAATCTCTTGGCTACC 394 381 177 Antisense
ACCTGCTTCAGGATTGTGTGGCTCA 13 89 311 Antisense
AAGCTCGAAATCTACAGCATCTTCT 598 149 361 Antisense
AGCATCTTCTTCAAGCTCTTTTGTC 144 79 376 Antisense
GCTCTTTTGTCGAACTGCGGAGCCC 72 299 390 Antisense
CCGCCGACACTGAGCTAGTCAACAG 452 261 413 Antisense
AATCGTCGAGCATTTTTCTAACAAC 574 167 438 Antisense
ACAACTTTCCGCAACCAGAGAGTCC 103 111 458 Antisense
Sequence Source Affymetrix Proprietary Database