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[175860_s_at]

Description F22B7.5 /REP_DB=WormBase Gene ID /WP=CE24911 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 55      6grp_high1_10_10 > 54      4grp_highest_8_8 > 44      4grp_high_8_8 > 08      6grp_highest_nonorm > 81      6grp_total > 45      4grp_total > 39      6grp_cgc > 35      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 170
1 2 3 4 range
A 294.2
(3)
P 		134.3
(-2.6)
P 		124.2
(-3)
P 		274.7
(2.3)
P 		170
B 196.3
(3)
P 		151.1
(-3)
P 		194.1
(2.7)
P 		164.3
(-1.2)
P 		45
C 292.1
(3)
P 		177
(-2.9)
P 		250.8
(0.9)
P 		175.8
(-3)
P 		116
avr 260.9
(3) 		154.1
(-3) 		189.7
(-1) 		204.9
(-0.1) 		107

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 211
1 2 3 4 5 6 range
A 197
(-1.1)
P 		292.9
(2.2)
P 		317.2
(3)
P 		249.3
(0.7)
P 		141.9
(-3)
P 		225.5
(-0.1)
P 		175
B 262.9
(0.1)
P 		266.4
(0.2)
P 		320
(3)
P 		256.6
(-0.3)
P 		203.5
(-3)
P 		252.3
(-0.5)
P 		117
C 352.5
(3)
P 		277.8
(0.5)
P 		267
(0.1)
P 		239.4
(-0.8)
P 		174.4
(-3)
P 		319.6
(1.9)
P 		178
avr 270.8
(1.6) 		279
(2) 		301.4
(3) 		248.4
(0.5) 		173.3
(-3) 		265.8
(1.3) 		128
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 78
1 2 3 4 range
A 107.1
(3)
P 		29.2
(-3)
P 		32.5
(-2.7)
P 		40.2
(-2.2)
P 		78
B 62.6
(3)
P 		37.3
(-2.5)
P 		45
(-0.8)
P 		35
(-3)
P 		28
C 96.7
(3)
P 		50.9
(-1.1)
P 		87.4
(2.2)
P 		29.6
(-3)
P 		67
avr 88.8
(3) 		39.1
(-2.5) 		55
(-0.8) 		34.9
(-3) 		54

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 94
1 2 3 4 5 6 range
A 36.3
(-3)
P 		104.2
(3)
P 		69.1
(-0.1)
P 		63.2
(-0.6)
P 		42.8
(-2.4)
P 		54.9
(-1.4)
P 		68
B 91.5
(2.4)
P 		88.7
(1.8)
P 		77.7
(-0.8)
P 		68
(-3)
P 		82.7
(0.4)
P 		94.1
(3)
P 		26
C 130.6
(3)
P 		84.8
(-0.3)
P 		68
(-1.6)
P 		48.2
(-3)
P 		56.4
(-2.4)
P 		107
(1.3)
P 		82
avr 86.1
(1.8) 		92.6
(3) 		71.6
(-0.8) 		59.8
(-3) 		60.6
(-2.9) 		85.3
(1.7) 		33
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175860_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.6120
Sequence Type Exemplar sequence
Representative Public ID F22B7.5 NCBI
Target Description F22B7.5 /REP_DB=WormBase Gene ID /WP=CE24911 /CHR=3 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_066501(11), NM_066500(10)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_066501 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-10) (dnj-10) mRNA, complete cds. 11/11 None
NM_066500 NCBI Caenorhabditis elegans DNaJ domain (prokaryotic heat shock protein) family member (dnj-10) (dnj-10) mRNA, complete cds. 10/11 None
SNAP00000003831 ENSEMBL cdna:SNAP chromosome:CEL140:III:8628837:8633237:1 10/11 None
GENEFINDER00000003840 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:8628860:8633237:1 11/11 None
F22B7.5a.2 ENSEMBL cdna:known chromosome:CEL140:III:8631061:8633395:1 gene:F22B7.5 11/11 None
F22B7.5a.1 ENSEMBL cdna:known chromosome:CEL140:III:8631068:8633395:1 gene:F22B7.5 11/11 None
F22B7.5b ENSEMBL cdna:known chromosome:CEL140:III:8631078:8633431:1 gene:F22B7.5 10/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8631072-8633078(+) 99.71 99.93
Public Domain and Genome References
Ensembl F22B7.5
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK68342 Dnaj domain (prokaryotic heat shock protein) protein 10, isoform a [Caenorhabditis elegans] ref|NP_498902.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-10) [Caenorhabditis elegans] sp|Q8TA83|DNJ10_CAEEL DnaJ homolog dnj-10 (DnaJ domain protein 10) 0.0
blast AAK68343 Dnaj domain (prokaryotic heat shock protein) protein 10, isoform b [Caenorhabditis elegans] ref|NP_498901.1| DNaJ domain (prokaryotic heat shock protein) family member (dnj-10) [Caenorhabditis elegans] 0.0
blast CAE75019 Hypothetical protein CBG22923 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 2.99999998801259E-12
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 9.99999999819959E-24
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 3.00000015723206E-23
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 8.99999968283527E-24
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.1E-33
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.9E-60
Pfam IPR001305 EMBL-EBI DnaJ central domain (CXXCXGXG) 1.1E-40
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.3E-21
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.9E-60
Pfam IPR003109 EMBL-EBI GoLoco 1.6E-9
Pfam IPR001305 EMBL-EBI DnaJ central domain (CXXCXGXG) 1.1E-40
Pfam IPR001623 EMBL-EBI Heat shock protein DnaJ, N-terminal 1.1E-33
Pfam IPR002939 EMBL-EBI Chaperone DnaJ, C-terminal 1.9E-60
Pfam IPR003109 EMBL-EBI GoLoco 1.6E-9
Pfam IPR001305 EMBL-EBI DnaJ central domain (CXXCXGXG) 1.1E-40
Sequence
Target Sequence 
>C. ELEGANS:175860_S_AT
gtgaaggtcaaaccgttcaacgacgtcaagtatcattcaatgtgccagctggaactaata
atggagatagtttgaagttccaagtggggaaaaatcaattatttgttcgtttcaacgttg
caccatctttgaaattccgacgtgagaaagatgatattcactgtgacgtagatatttctc
tggctcaagctgttcttggtggtactgtaaaggttcctggaattaatggagatacatatg
ttcatattccggcaggaactggcagtcacactaaaatgagattaacaggaaaaggagtaa
aacgattgcattcttacggaaatggagatcaatatatgcatattaaagtaacggttccga
aatatttgacagccgaacagaaacaaattatgttggcttgggctgcgacggaacagctga
aagatggaaccatcaaaggattggagaaaaatcagaaaaccgaggagaaggagacgaaga
aaaatgaggaaaagaagtctgaaggtgcatcagaatcacaaaaacggagaagtgagccag
tagctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAAGGTCAAACCGTTCAACGACG 693 471 761 Antisense
GTTCAACGACGTCAAGTATCATTCA 347 443 775 Antisense
TGTTCGTTTCAACGTTGCACCATCT 354 561 864 Antisense
ATTCACTGTGACGTAGATATTTCTC 700 11 916 Antisense
GATATTTCTCTGGCTCAAGCTGTTC 383 427 931 Antisense
TGGTGGTACTGTAAAGGTTCCTGGA 233 553 957 Antisense
ATTCCGGCAGGAACTGGCAGTCACA 187 9 1006 Antisense
GGTTCCGAAATATTTGACAGCCGAA 439 507 1113 Antisense
AATTATGTTGGCTTGGGCTGCGACG 590 175 1146 Antisense
GCTTGGGCTGCGACGGAACAGCTGA 257 305 1156 Antisense
AAACGGAGAAGTGAGCCAGTAGCTG 373 117 1282 Antisense
Sequence Source Affymetrix Proprietary Database