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[175854_s_at]

Description M70.3B /REP_DB=WormBase Gene ID /WP=CE25959 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Cluster 6grp_total > 86      4grp_total > 05      6grp_total_sub > Increase      6grp_rule_based > S6      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 102
1 2 3 4 range
A 74.5
(0)
A 		23.1
(-3)
A 		58.4
(-0.9)
A 		125.3
(3)
A 		102
B 75.2
(3)
A 		42.7
(-3)
A 		44.5
(-2.7)
A 		44.3
(-2.7)
A 		33
C 51.1
(-3)
A 		72.9
(-1.2)
A 		60.9
(-2.2)
A 		123.6
(3)
A 		73
avr 66.9
(-0.6) 		46.2
(-3) 		54.6
(-2) 		97.7
(3) 		52

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 171
1 2 3 4 5 6 range
A 18.3
(-2.8)
A 		16.8
(-2.8)
A 		11.8
(-3)
A 		20.3
(-2.7)
A 		53.6
(-1.5)
A 		183
(3)
P 		171
B 19.3
(-3)
A 		18.9
(-3)
A 		43.6
(-1.5)
A 		90.9
(1.4)
A 		87.5
(1.2)
A 		116.4
(3)
A 		98
C 16.9
(-3)
A 		29.1
(-2.4)
A 		76.4
(0.1)
A 		82.4
(0.4)
A 		91.8
(0.8)
A 		133.7
(3)
P 		117
avr 18.2
(-3) 		21.6
(-2.8) 		43.9
(-1.8) 		64.5
(-0.8) 		77.6
(-0.2) 		144.4
(3) 		126
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 22
1 2 3 4 range
A 27.1
(3)
A 		5
(-3)
A 		15.3
(-0.2)
A 		18.3
(0.6)
A 		22
B 24
(3)
A 		10.5
(-2.5)
A 		10.3
(-2.6)
A 		9.4
(-3)
A 		15
C 16.9
(-3)
A 		21
(2.7)
A 		21.2
(3)
A 		20.8
(2.4)
A 		4
avr 22.7
(3) 		12.2
(-3) 		15.6
(-1.1) 		16.2
(-0.7) 		11

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 42
1 2 3 4 5 6 range
A 3.4
(-2.9)
A 		6
(-2.5)
A 		2.6
(-3)
A 		5.1
(-2.6)
A 		16.2
(-1.1)
A 		44.6
(3)
P 		42
B 6.7
(-2.9)
A 		6.3
(-3)
A 		10.6
(-2.3)
A 		24.1
(-0.1)
A 		35.5
(1.7)
A 		43.4
(3)
A 		37
C 6.3
(-3)
A 		8.9
(-2.6)
A 		19.4
(-1)
A 		16.6
(-1.4)
A 		29.7
(0.6)
A 		44.8
(3)
P 		39
avr 5.5
(-3) 		7.1
(-2.8) 		10.9
(-2.2) 		15.3
(-1.5) 		27.1
(0.3) 		44.3
(3) 		39
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175854_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.11198
Sequence Type Exemplar sequence
Representative Public ID M70.3B NCBI
Target Description M70.3B /REP_DB=WormBase Gene ID /WP=CE25959 /SUBMIT=ST.LOUIS /CHR=4 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_067779(11), NM_067778(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_067779 NCBI Caenorhabditis elegans M70.3b (M70.3) mRNA, complete cds. 11/11 None
NM_067778 NCBI Caenorhabditis elegans M70.3a (M70.3) mRNA, complete cds. 11/11 None
SNAP00000012653 ENSEMBL cdna:SNAP chromosome:CEL140:IV:2237716:2241561:-1 11/11 None
GENEFINDER00000012660 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:2237716:2242392:-1 11/11 None
M70.3b ENSEMBL cdna:known chromosome:CEL140:IV:2237682:2241588:-1 gene:M70.3 11/11 None
M70.3a ENSEMBL cdna:known chromosome:CEL140:IV:2237716:2242416:-1 gene:M70.3 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:2237721-2241567(-) 100.0 100.0
Public Domain and Genome References
Ensembl M70.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK71389 Hypothetical protein M70.3b [Caenorhabditis elegans] ref|NP_500180.1| M70.3b [Caenorhabditis elegans] 0.0
blast AAK71388 Hypothetical protein M70.3a [Caenorhabditis elegans] ref|NP_500179.1| M70.3a [Caenorhabditis elegans] 0.0
blast AAC04435 Hypothetical protein M70.1 [Caenorhabditis elegans] ref|NP_500182.1| M70.1 [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 7.9E-16
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 7.9E-16
Pfam IPR003125 EMBL-EBI Protein of unknown function WSN 7.9E-16
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_500180.1 2 5-24,780-802
NP_500179.1 2 5-24,809-831
M70.3b 2 5-24,780-802
M70.3a 2 5-24,809-831
SNAP00000012653 2 5-24,760-782
GENEFINDER00000012660 2 5-24,809-831
Sequence
Target Sequence 
>C. ELEGANS:175854_S_AT
tgttacccttacctatatctcaaaagccaaagcttgacgagctcaagagttcactccaaa
gtgttgagccattggatttgcaattttccaaattcaatggagcattgaaggacatttcaa
caactttcaacgggcttcaattggtgttctcaagtggaaaaacgggaaataaaactgttt
cgaaaggcccagctgcagtgaagaaagcgcaggcttcaactccacctccagcatcagaca
attccatgatgatcatcattgctgtctcggttgcagttgtgctcgtcgcatccatcctga
ttgtcgctggtttgtggttctttggatttttgccgttttgcccgagaaagaagaactcga
agactgccggaagagatcaaaaggggaaaagcaagaagactaagaagggcaagagcaagt
cgggcaaggagccggtgccaatttccgaagcaacaaactcgacaccctctggggaagatg
gatccaagattgacactattagtaaatcgaagacggagccaattcgagcaccaccaaagg
ac

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTTACCCTTACCTATATCTCAAAA 266 559 2096 Antisense
TCAACAACTTTCAACGGGCTTCAAT 182 629 2212 Antisense
AACTGTTTCGAAAGGCCCAGCTGCA 158 141 2268 Antisense
ACCTCCAGCATCAGACAATTCCATG 509 87 2319 Antisense
GATGATCATCATTGCTGTCTCGGTT 74 411 2343 Antisense
ATCCATCCTGATTGTCGCTGGTTTG 423 39 2385 Antisense
TGTGGTTCTTTGGATTTTTGCCGTT 666 553 2408 Antisense
TTTGCCGTTTTGCCCGAGAAAGAAG 312 669 2424 Antisense
CAAGGAGCCGGTGCCAATTTCCGAA 292 181 2520 Antisense
AAACTCGACACCCTCTGGGGAAGAT 250 127 2550 Antisense
GCCAATTCGAGCACCACCAAAGGAC 9 277 2613 Antisense
Sequence Source Affymetrix Proprietary Database