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[175850_at]

Description R05G9.C /REP_DB=WormBase Gene ID /WP=CE21109 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster 6grp_highest_10_10 > 21      6grp_high1_10_10 > 12      4grp_highest_8_8 > 40      4grp_high_8_8 > 43      6grp_highest_nonorm > 31      6grp_total > 23      4grp_total > 53      6grp_highest_sub > De      6grp_high1_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1036
1 2 3 4 range
A 1220.3
(3)
P 		926.7
(1)
P 		728.2
(-0.4)
P 		353.9
(-3)
P 		866
B 1229.5
(3)
P 		1132.4
(2.4)
P 		621.2
(-1)
P 		309
(-3)
P 		921
C 1345.4
(3)
P 		1178.6
(2)
P 		903.6
(0.3)
P 		376.1
(-3)
P 		969
avr 1265.1
(3) 		1079.2
(1.8) 		751
(-0.4) 		346.3
(-3) 		919

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1445
1 2 3 4 5 6 range
A 1759.9
(3)
P 		1250.5
(0.9)
P 		1324.2
(1.2)
P 		711.9
(-1.4)
P 		490.3
(-2.3)
P 		315.2
(-3)
P 		1445
B 1522.5
(3)
P 		1171.4
(1.1)
P 		975.6
(0.1)
P 		888.4
(-0.4)
P 		532.5
(-2.3)
P 		406.7
(-3)
P 		1116
C 1103.9
(3)
P 		1068.3
(2.7)
P 		979.8
(2)
P 		889.1
(1.3)
P 		405.6
(-2.5)
P 		341.1
(-3)
P 		763
avr 1462.1
(3) 		1163.4
(1.4) 		1093.2
(1) 		829.8
(-0.4) 		476.1
(-2.3) 		354.3
(-3) 		1108
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 394
1 2 3 4 range
A 444.3
(3)
P 		201.2
(-0.7)
P 		190.4
(-0.9)
P 		51.8
(-3)
P 		393
B 392.1
(3)
P 		279.7
(0.9)
P 		144
(-1.6)
P 		65.8
(-3)
P 		326
C 445.4
(3)
P 		338.9
(1.3)
P 		314.9
(1)
P 		63.4
(-3)
P 		382
avr 427.3
(3) 		273.3
(0.5) 		216.4
(-0.4) 		60.3
(-3) 		367

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 453
1 2 3 4 5 6 range
A 324.4
(1)
P 		444.8
(3)
P 		288.4
(0.5)
P 		180.6
(-1.3)
P 		147.9
(-1.8)
P 		76.7
(-3)
P 		368
B 529.8
(3)
P 		389.9
(0.8)
P 		236.9
(-1.6)
P 		235.3
(-1.7)
P 		216.4
(-2)
P 		151.7
(-3)
P 		378
C 409.2
(3)
P 		326
(1.3)
P 		249.4
(-0.3)
P 		178.8
(-1.7)
P 		131.2
(-2.7)
P 		114.2
(-3)
P 		295
avr 421.1
(3) 		386.9
(2.3) 		258.2
(-0.2) 		198.2
(-1.4) 		165.2
(-2) 		114.2
(-3) 		307
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175850_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18881
Sequence Type Exemplar sequence
Representative Public ID R05G9.C NCBI
Target Description R05G9.C /REP_DB=WormBase Gene ID /WP=CE21109 /CHR=2 /FEA=Sanger Annotation /DEF=(ST.LOUIS)
Cluster Evidence This cluster is supported by a Sanger Annotation.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027097(11), NM_001027096(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027097 NCBI Caenorhabditis elegans R05G9.2b (R05G9.2) mRNA, complete cds. 11/11 None
NM_001027096 NCBI Caenorhabditis elegans R05G9.2a (R05G9.2) mRNA, complete cds. 11/11 None
GENEFINDER00000037112 ENSEMBL cdna:GeneFinder chromosome:CEL140:II:6493810:6497655:-1 11/11 None
R05G9.2b ENSEMBL cdna:known chromosome:CEL140:II:6493697:6498577:-1 gene:R05G9.2 11/11 None
R05G9.2a ENSEMBL cdna:known chromosome:CEL140:II:6493810:6497655:-1 gene:R05G9.2 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000037104 7/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:6493806-6497652(-) 100.0 100.0
Public Domain and Genome References
Ensembl R05G9.2
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAU20842 Hypothetical protein R05G9.2b [Caenorhabditis elegans] ref|NP_001022268.1| R05G9.2b [Caenorhabditis elegans] 1.0E-151
blast AAK68392 Hypothetical protein R05G9.2a [Caenorhabditis elegans] ref|NP_001022267.1| R05G9.2a [Caenorhabditis elegans] 1.0E-138
blast AAU20842 Hypothetical protein R05G9.2b [Caenorhabditis elegans] ref|NP_001022268.1| R05G9.2b [Caenorhabditis elegans] 1.0E-138
Protein Domains
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001022268.1 5 47-69,145-164,174-196,217-239,270-292
NP_001022267.1 5 25-47,123-142,152-174,195-217,248-270
R05G9.2b 5 47-69,145-164,174-196,217-239,270-292
R05G9.2a 5 25-47,123-142,152-174,195-217,248-270
GENEFINDER00000037112 5 25-47,123-142,152-174,195-217,248-270
Sequence
Target Sequence 
>C. ELEGANS:175850_AT
ctccttctcgtcgtgaatggtcttggattagcagttttaattttgcgtattctcttcttt
tgacgatcggtggtggattcaaagttccagctactgttggttcccagatatttgctgtat
tctattgtctaattggtattccattgttctacagtactctgatactcatcgtctgtcgat
tggtatctccagttttaaagtggccatcactaactcgttcgcgtcgtttcctgctcattc
ttgcggcctttggtctctttattctctgggcaatcctcattggcctttgtctctattatc
aggtgatcaatgacttctggcttagcattttacatgctttcactggatctcttactgtcc
aaattccaagccccgcacaaattacaacttgcggaatcctgttcttgaactttgctgcta
ccatttcggtttctcttcttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTCCTTCTCGTCGTGAATGGTCTTG 519 233 551 Antisense
GTATTCTCTTCTTTTGACGATCGGT 690 451 597 Antisense
TTCCAGCTACTGTTGGTTCCCAGAT 128 701 635 Antisense
TACTCTGATACTCATCGTCTGTCGA 162 647 705 Antisense
TTATTCTCTGGGCAATCCTCATTGG 501 671 809 Antisense
CATTGGCCTTTGTCTCTATTATCAG 322 217 828 Antisense
GGTGATCAATGACTTCTGGCTTAGC 229 501 852 Antisense
TACATGCTTTCACTGGATCTCTTAC 119 645 881 Antisense
TTCCAAGCCCCGCACAAATTACAAC 15 699 914 Antisense
TGTTCTTGAACTTTGCTGCTACCAT 556 561 950 Antisense
CTACCATTTCGGTTTCTCTTCTTAT 379 223 968 Antisense
Sequence Source Affymetrix Proprietary Database