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[175838_at]

Description g4164605 /REP_DB=GenBank Identifier /GB=AF116529.1 /PROTEIN_GB=AAD05570.1 /CHR=1 /FEA=Mapped Transcript /DEF=synthetic multivulva protein LIN-35 Rb
Cluster 6grp_highest_10_10 > 95      6grp_high1_10_10 > 59      4grp_highest_8_8 > 26      4grp_high_8_8 > 59      6grp_highest_nonorm > 91      6grp_total > 49      4grp_total > 63      6grp_highest_sub > Cu      6grp_cgc > 19      6grp_high1_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 249
1 2 3 4 range
A 133.1
(1.2)
P 		121.1
(0.6)
P 		170
(3)
P 		48.5
(-3)
P 		122
B 181.7
(0.1)
P 		139.8
(-1.7)
P 		251
(3)
P 		109.7
(-3)
P 		141
C 269.1
(1.7)
P 		165.4
(-3)
P 		297.3
(3)
P 		165.8
(-3)
P 		132
avr 194.6
(1) 		142.1
(-1.4) 		239.4
(3) 		108
(-3) 		131

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 180
1 2 3 4 5 6 range
A 203.6
(-0.3)
P 		240
(2.9)
P 		190.5
(-1.5)
P 		173.2
(-3)
P 		240.9
(3)
P 		232.1
(2.2)
P 		68
B 178.3
(-2.3)
P 		219.3
(-0.2)
P 		179
(-2.3)
P 		164.7
(-3)
P 		281.1
(3)
P 		240.6
(0.9)
P 		116
C 304.9
(2.9)
P 		194.9
(-0.8)
P 		129
(-3)
P 		161.5
(-1.9)
P 		159.2
(-2)
P 		308.8
(3)
P 		180
avr 228.9
(1) 		218.1
(0.3) 		166.2
(-3) 		166.5
(-3) 		227.1
(0.9) 		260.5
(3) 		94
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 97
1 2 3 4 range
A 48.5
(3)
P 		26.3
(-0.2)
P 		44.4
(2.4)
P 		7.1
(-3)
P 		41
B 57.9
(2.9)
P 		34.5
(-1.1)
P 		58.2
(3)
P 		23.4
(-3)
P 		35
C 89.1
(1.9)
P 		47.6
(-1.4)
P 		103.6
(3)
P 		27.9
(-3)
P 		76
avr 65.2
(2.6) 		36.1
(-1) 		68.7
(3) 		19.5
(-3) 		49

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 82
1 2 3 4 5 6 range
A 37.5
(-3)
P 		85.4
(3)
P 		41.5
(-2.5)
P 		43.9
(-2.2)
P 		72.7
(1.4)
P 		56.5
(-0.6)
P 		48
B 62
(-1.4)
P 		73
(-0.5)
P 		43.5
(-3)
P 		43.6
(-3)
P 		114.2
(3)
P 		89.7
(0.9)
P 		71
C 113
(3)
P 		59.5
(-1)
P 		32.8
(-3)
P 		32.5
(-3)
P 		51.5
(-1.6)
P 		103.4
(2.3)
P 		81
avr 70.8
(1.3) 		72.6
(1.6) 		39.3
(-3) 		40
(-2.9) 		79.5
(2.5) 		83.2
(3) 		44
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175838_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19675
Sequence Type Exemplar sequence
Representative Public ID 4164605 NCBI
Target Description g4164605 /REP_DB=GenBank Identifier /GB=AF116529.1 /PROTEIN_GB=AAD05570.1 /CHR=1 /FEA=Mapped Transcript /DEF=synthetic multivulva protein LIN-35 Rb
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059285(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059285 NCBI Caenorhabditis elegans abnormal cell LINeage family member (lin-35) (lin-35) mRNA, complete cds. 11/11 A 
C32F10.2 ENSEMBL cdna:known chromosome:CEL140:I:5815845:5823773:1 gene:C32F10.2 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:5815842-5823773(+) 97.92 100.0
Public Domain and Genome References
Gene Title
Gene Symbol lin-35
Ensembl C32F10.2
Entrez Gene 172249 Entrez gene
SwissProt O01682 EMBL-EBI
WormBase CE24823 Wormbase
Functional Annotations
Ortholog
ID Title Organism
CANINE:1604598_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE:1592688_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE:1584401_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.12061.1.A1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.18203.1.S1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFA.19796.1.S1_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CANINE_2:CFAAFFX.15185.1.S1_S_AT similar to Retinoblastoma-like protein 2 (130 kDa retinoblastoma-associated protein) (PRB2) (P130) (RBR-2) cfa
CHICKEN:GGA.7958.3.S1_AT similar to retinoblastoma-like 2 (p130) gga
CHICKEN:GGAAFFX.25929.1.S1_AT similar to retinoblastoma-like 2 (p130) gga
CHICKEN:GGAAFFX.25929.3.S1_S_AT similar to retinoblastoma-like 2 (p130) gga
HU35KSUBD:RC_F10323_F_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:1986_AT retinoblastoma-like 2 (p130) hs
HC-G110:1986_AT retinoblastoma-like 2 (p130) hs
HU35KSUBC:RC_W58597_S_AT retinoblastoma-like 2 (p130) hs
HUGENEFL:X76061_AT retinoblastoma-like 2 (p130) hs
U133_X3P:HS.79362.0.S3_3P_AT retinoblastoma-like 2 (p130) hs
U133_X3P:HS.79362.0.S1_3P_AT retinoblastoma-like 2 (p130) hs
HG-U133A_2:212331_AT retinoblastoma-like 2 (p130) hs
HG-FOCUS:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133_PLUS_2:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133A:212331_AT retinoblastoma-like 2 (p130) hs
HG-U133A_2:212332_AT retinoblastoma-like 2 (p130) hs
HG-U133_PLUS_2:212332_AT retinoblastoma-like 2 (p130) hs
HG-U133A:212332_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:32597_AT retinoblastoma-like 2 (p130) hs
HG-U95D:79041_AT retinoblastoma-like 2 (p130) hs
HG-U95AV2:32596_AT Retinoblastoma-like 2 (p130) hs
MOUSE430_2:1425981_A_AT retinoblastoma-like 2 mm
MOE430A:1425981_A_AT retinoblastoma-like 2 mm
MOUSE430A_2:1425981_A_AT retinoblastoma-like 2 mm
MU11KSUBA:U36799_S_AT retinoblastoma-like 2 mm
MG-U74AV2:95617_AT retinoblastoma-like 2 mm
MOE430A:1418146_A_AT retinoblastoma-like 2 mm
MOUSE430A_2:1418146_A_AT retinoblastoma-like 2 mm
MOUSE430_2:1418146_A_AT retinoblastoma-like 2 mm
MU19KSUBB:TC30332_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC30333_S_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC32860_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
MU19KSUBB:TC32860_G_AT Retinoblastoma-like 2, mRNA (cDNA clone MGC:18400 IMAGE:4240592) mm
RG-U34A:RC_AI227715_AT retinoblastoma-like 2 rn
RAE230A:1368403_AT retinoblastoma-like 2 rn
RAT230_2:1368403_AT retinoblastoma-like 2 rn
RAE230B:1378190_AT Retinoblastoma-like 2 rn
RAT230_2:1378190_AT Retinoblastoma-like 2 rn
RAT230_2:1395140_AT Retinoblastoma-like 2 rn
RAE230B:1395140_AT Retinoblastoma-like 2 rn
RG-U34B:RC_AA945871_AT Retinoblastoma-like 2 rn
Protein Similarities
Method ID Description E-Value
blast AAC24266 Abnormal cell lineage protein 35 [Caenorhabditis elegans] ref|NP_491686.1| abnormal cell LINeage family member (lin-35) [Caenorhabditis elegans] gb|AAD05570.1| synthetic multivulva protein LIN-35 Rb [Caenorhabditis elegans] 0.0
blast CAE60456 Hypothetical protein CBG04064 [Caenorhabditis briggsae] 0.0
blast EAL31534 GA20332-PA [Drosophila pseudoobscura] 2.0E-28
Protein Domains
Database ID Description E-Value
Pfam IPR002720 EMBL-EBI Retinoblastoma-associated protein, A-box 1.7E-96
Pfam IPR002719 EMBL-EBI Retinoblastoma-associated protein, B-box 1.4E-90
Sequence
Target Sequence 
>C. ELEGANS:175838_AT
gtgaatactttgaactttttaatgcatttttgattaatcatttagtacttcttttctcgt
ctatttttttatcttttccttcaaattcaggcaagtaattatactttccatttctaattg
attgcttcaaaatagacgtctagttatattcaaaacaatcccccttttgaattggaatct
tcaaatatcgtattaaatattaatattgtaatcatttttcacaatcccccatgccattat
tgttactgattttttctctcttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GTGAATACTTTGAACTTTTTAATGC 126 481 2933 Antisense
ATCATTTAGTACTTCTTTTCTCGTC 514 29 2969 Antisense
TTAGTACTTCTTTTCTCGTCTATTT 229 681 2974 Antisense
TTTCCTTCAAATTCAGGCAAGTAAT 399 669 3007 Antisense
AGTAATTATACTTTCCATTTCTAAT 678 59 3026 Antisense
TTTCCATTTCTAATTGATTGCTTCA 611 669 3037 Antisense
GATTGCTTCAAAATAGACGTCTAGT 386 429 3052 Antisense
CAATCCCCCTTTTGAATTGGAATCT 355 177 3088 Antisense
ATTGGAATCTTCAAATATCGTATTA 623 3 3103 Antisense
CAATCCCCCATGCCATTATTGTTAC 570 179 3154 Antisense
ATTGTTACTGATTTTTTCTCTCTTT 490 5 3171 Antisense
Sequence Source GenBank