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[175837_s_at]

Description g2104696 /REP_DB=GenBank Identifier /GB=U93847.1 /PROTEIN_GB=AAB58269.1 /CHR=3 /FEA=Mapped Transcript /DEF=elongation factor-2 kinase EFK-1B isoform
Cluster 6grp_highest_10_10 > 59      6grp_high1_10_10 > 95      4grp_highest_8_8 > 30      4grp_high_8_8 > 05      6grp_highest_nonorm > 97      6grp_total > 75      4grp_total > 04      6grp_highest_sub > In      6grp_cgc > 67      6grp_high1_sub > In      6grp_cgc_sub > In      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1306
1 2 3 4 range
A 679.2
(-1.6)
P 		377.3
(-3)
P 		403.8
(-2.9)
P 		1683.4
(3)
P 		1306
B 596.1
(-2.2)
P 		464.2
(-3)
P 		457.8
(-3)
P 		1473
(3)
P 		1015
C 712.2
(-1.6)
P 		550.7
(-2.6)
P 		494.9
(-3)
P 		1400.2
(3)
P 		905
avr 662.5
(-1.8) 		464.1
(-2.9) 		452.2
(-3) 		1518.9
(3) 		1067

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1365
1 2 3 4 5 6 range
A 460.1
(-3)
P 		619.2
(-2.3)
P 		715.1
(-1.9)
P 		1278.3
(0.6)
P 		883.6
(-1.1)
P 		1825.3
(3)
P 		1365
B 520.7
(-3)
P 		514.1
(-3)
P 		766
(-1.5)
P 		1171.4
(1)
P 		981.6
(-0.1)
P 		1491.8
(3)
P 		978
C 818.1
(-1.2)
P 		472.4
(-3)
P 		933.1
(-0.6)
P 		1215.9
(0.8)
P 		1039.7
(-0.1)
P 		1633.3
(3)
P 		1161
avr 599.6
(-2.7) 		535.2
(-3) 		804.7
(-1.5) 		1221.9
(0.7) 		968.3
(-0.7) 		1650.1
(3) 		1115
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 232
1 2 3 4 range
A 247.3
(3)
P 		81.9
(-3)
P 		105.6
(-2.1)
P 		246.4
(3)
P 		165
B 190.1
(-0.6)
P 		114.6
(-2.8)
P 		106.1
(-3)
P 		313.9
(3)
P 		208
C 235.8
(3)
P 		158.3
(-3)
P 		172.5
(-1.9)
P 		236.1
(3)
P 		78
avr 224.4
(1.3) 		118.3
(-3) 		128.1
(-2.6) 		265.5
(3) 		147

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 472
1 2 3 4 5 6 range
A 84.8
(-3)
P 		220.2
(-0.7)
P 		155.7
(-1.8)
P 		324.2
(1)
P 		266.6
(0)
P 		444.4
(3)
P 		360
B 181.2
(-2.8)
P 		171.1
(-3)
P 		186
(-2.8)
P 		310.3
(-0.8)
P 		398.8
(0.5)
P 		556.3
(3)
P 		385
C 303.2
(-0.6)
P 		144.1
(-3)
P 		237.5
(-1.6)
P 		244.5
(-1.5)
P 		336.4
(-0.1)
P 		546.8
(3)
P 		403
avr 189.7
(-2.8) 		178.5
(-3) 		193.1
(-2.7) 		293
(-1) 		333.9
(-0.2) 		515.8
(3) 		337
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175837_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19787
Sequence Type Exemplar sequence
Representative Public ID 2104696 NCBI
Target Description g2104696 /REP_DB=GenBank Identifier /GB=U93847.1 /PROTEIN_GB=AAB58269.1 /CHR=3 /FEA=Mapped Transcript /DEF=elongation factor-2 kinase EFK-1B isoform
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001027401(11), NM_001027402(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001027401 NCBI Caenorhabditis elegans Elongation Factor Kinase family member (efk-1) (efk-1) mRNA, complete cds. 11/11 None
NM_001027402 NCBI Caenorhabditis elegans Elongation Factor Kinase family member (efk-1) (efk-1) mRNA, complete cds. 11/11 None
SNAP00000011646 ENSEMBL cdna:SNAP chromosome:CEL140:III:6165229:6168470:-1 11/11 None
GENEFINDER00000011663 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:6165229:6168470:-1 10/11 None
F42A10.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:6164840:6168494:-1 gene:F42A10.4 11/11 None
F42A10.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:6164840:6168487:-1 gene:F42A10.4 11/11 None
F42A10.4b ENSEMBL cdna:known chromosome:CEL140:III:6165229:6168470:-1 gene:F42A10.4 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:6165227-6168469(-) 100.0 100.0
Public Domain and Genome References
Ensembl F42A10.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAN63383 Elongation factor kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001022572.1| Elongation Factor Kinase family member (efk-1) [Caenorhabditis elegans] sp|O01991|EF2K_CAEEL Elongation factor 2 kinase (eEF-2 kinase) (eEF-2K) (Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase) gb|AAB58268.1| elongation factor-2 kinase EFK-1A isoform [Caenorhabditis elegans] 0.0
blast AAN63384 Elongation factor kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001022573.1| Elongation Factor Kinase family member (efk-1) [Caenorhabditis elegans] gb|AAB58269.1| elongation factor-2 kinase EFK-1B isoform [Caenorhabditis elegans] 0.0
blast T30958 hypothetical protein F42A10.4 - Caenorhabditis elegans 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004166 EMBL-EBI MHCK/EF2 kinase domain 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175837_S_AT
aatgacgctcgtgtaccaatcaaatacgacaagcagtctgcaattttccatttggatatc
gctcggaagtgtggaatccttgaggctgtgctaacatcggctcatattgttctcggatta
ccacatgaattgttgaaagaagtcaccgttgatgatctgtttcctaatgggtttggagaa
caggaaaatggaattcgagacctagaagagttcggctccgatctgatggaaattgctgca
gagatgggtgataagggtgcaatgctgtacatggcacacgcttatgaaactggtcagcat
ctcggaccgaatcgaagaacggattataagaaatcgattgattggtatcaacgcgtcgtt
ggattccaagaagaagaagaacttgactctgattgtggaaaaacgacattctcctcattt
gctccactgactcgtcacgagattctagccaaaatggctgaaatgtacaaagagggaggt
tatggcctgaatcaagacttcgaacg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AATGACGCTCGTGTACCAATCAAAT 284 155 1687 Antisense
GAATCCTTGAGGCTGTGCTAACATC 530 329 1760 Antisense
GGCTCATATTGTTCTCGGATTACCA 493 539 1785 Antisense
GTTGATGATCTGTTTCCTAATGGGT 329 437 1834 Antisense
GACCTAGAAGAGTTCGGCTCCGATC 477 383 1885 Antisense
GCAATGCTGTACATGGCACACGCTT 72 323 1945 Antisense
ATGGCACACGCTTATGAAACTGGTC 655 51 1957 Antisense
GAAACTGGTCAGCATCTCGGACCGA 639 357 1972 Antisense
GGTATCAACGCGTCGTTGGATTCCA 110 505 2030 Antisense
TGCTCCACTGACTCGTCACGAGATT 269 581 2106 Antisense
ATGGCCTGAATCAAGACTTCGAACG 675 53 2168 Antisense
Sequence Source GenBank