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[175834_s_at]

Description g3063644 /REP_DB=GenBank Identifier /GB=AF057165.1 /PROTEIN_GB=AAC14129.1 /CHR=3 /FEA=Mapped Transcript /DEF=putative serine-threonine protein kinase
Cluster 6grp_highest_10_10 > 97      6grp_high1_10_10 > 78      4grp_highest_8_8 > 08      4grp_high_8_8 > 53      6grp_highest_nonorm > 98      6grp_total > 79      4grp_total > 59      6grp_highest_sub > Cu      6grp_cgc > 49      6grp_high1_sub > Cu      6grp_cgc_sub > Da      4grp_rule_based > L2      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 878
1 2 3 4 range
A 891
(0.7)
P
1163.9
(2.7)
P
1210.5
(3)
P
384.3
(-3)
P
826
B 969.5
(1.1)
P
1221.9
(3)
P
1125.3
(2.3)
P
444.3
(-3)
P
778
C 778.9
(-0.3)
P
1262.4
(3)
P
1148.5
(2.2)
P
389.1
(-3)
P
873
avr 879.8
(0.5)
1216.1
(3)
1161.4
(2.6)
405.9
(-3)
810

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1605
1 2 3 4 5 6 range
A 1034.5
(-0.7)
P
672.7
(-2.2)
P
474.2
(-3)
P
622
(-2.4)
P
1413.9
(0.9)
P
1910.3
(3)
P
1436
B 791.3
(-2)
P
695.5
(-2.3)
P
519.5
(-3)
P
849.2
(-1.7)
P
1137.8
(-0.6)
P
2079.6
(3)
P
1560
C 757.8
(-2.1)
P
622.8
(-2.7)
P
560.2
(-3)
P
797.8
(-1.9)
P
1177.4
(-0.1)
P
1853.6
(3)
P
1293
avr 861.2
(-1.6)
663.7
(-2.4)
518
(-3)
756.3
(-2)
1243
(0)
1947.8
(3)
1430
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 344
1 2 3 4 range
A 324.4
(3)
P
252.7
(1.4)
P
316.4
(2.8)
P
56.3
(-3)
P
268
B 309.2
(3)
P
301.8
(2.8)
P
260.8
(1.6)
P
94.7
(-3)
P
215
C 257.8
(0.4)
P
362.9
(2.3)
P
400.3
(3)
P
65.6
(-3)
P
335
avr 297.1
(2.3)
305.8
(2.5)
325.8
(3)
72.2
(-3)
254

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 672
1 2 3 4 5 6 range
A 190.7
(-1.6)
P
239.3
(-0.7)
P
103.3
(-3)
P
157.8
(-2.1)
P
426.7
(2.4)
P
465.1
(3)
P
362
B 275.3
(-1.6)
P
231.5
(-2)
P
126.1
(-3)
P
224.9
(-2.1)
P
462.3
(0.1)
P
775.5
(3)
P
649
C 280.9
(-1.3)
P
190
(-2.4)
P
142.6
(-3)
P
160.4
(-2.8)
P
381
(-0)
P
620.5
(3)
P
478
avr 249
(-1.5)
220.3
(-1.8)
124
(-3)
181
(-2.3)
423.3
(0.6)
620.4
(3)
496
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175834_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19818
Sequence Type Exemplar sequence
Representative Public ID 3063644 NCBI
Target Description g3063644 /REP_DB=GenBank Identifier /GB=AF057165.1 /PROTEIN_GB=AAC14129.1 /CHR=3 /FEA=Mapped Transcript /DEF=putative serine-threonine protein kinase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026002(11), NM_001026003(11)
Transcript Assignments
Representative
Transcript
Description Matching Probes Related Probesets by Grade
NM_001026002 NCBI Caenorhabditis elegans POLO Kinase family member (plk-1) (plk-1) mRNA, complete cds. 11/11 None
NM_001026003 NCBI Caenorhabditis elegans POLO Kinase family member (plk-1) (plk-1) mRNA, complete cds. 11/11 None
C14B9.4a.2 ENSEMBL cdna:known chromosome:CEL140:III:8101268:8103992:1 gene:C14B9.4 11/11 None
C14B9.4b ENSEMBL cdna:known chromosome:CEL140:III:8101269:8103997:1 gene:C14B9.4 11/11 A 
C14B9.4a.1 ENSEMBL cdna:known chromosome:CEL140:III:8101269:8103992:1 gene:C14B9.4 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:8101266-8103995(+) 100.0 100.0
Public Domain and Genome References
Ensembl C14B9.4
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAA27947 Polo kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001021173.1| POLO Kinase family member (plk-1) [Caenorhabditis elegans] gb|AAC34661.1| polo-like kinase-1; PLK-1 [Caenorhabditis elegans] 0.0
blast AAM22025 Polo kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001021174.1| POLO Kinase family member (plk-1) [Caenorhabditis elegans] gb|AAC14129.1| putative serine/threonine protein kinase [Caenorhabditis elegans] sp|P34331|PLK1_CAEEL Serine/threonine-protein kinase plk-1 (Polo-like kinase-1) 0.0
blast CAE64506 Hypothetical protein CBG09233 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-59
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 5.0E-71
hanks 2.1.8 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; DdMLCK 6.0E-73
Protein Domains
Database ID Description E-Value
Pfam IPR000959 EMBL-EBI POLO box duplicated region 2.3E-33
Pfam IPR000959 EMBL-EBI POLO box duplicated region 3.0E-24
Pfam IPR000719 EMBL-EBI Protein kinase 3.0E-82
Pfam IPR000959 EMBL-EBI POLO box duplicated region 2.3E-33
Pfam IPR000959 EMBL-EBI POLO box duplicated region 3.0E-24
Pfam IPR000719 EMBL-EBI Protein kinase 1.6E-78
Sequence
Target Sequence
>C. ELEGANS:175834_S_AT
gatagatccgtatgtgctctttgccacttccatgaatttgcttacatctcctgattaatt
ccaagaagtattattctatacatttaacccgtactcttcatttattgttgctgtttttca
tcgacccttttagtcccatgtttcagcatccaacatgtctttattccccgtcctcgatct
cccgtagctcatttcattgtaaatactgatgagacccgctgtagccctttttcattcgtt
tgtattcatgggcgaggcgat


BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position
Strandedness
GATAGATCCGTATGTGCTCTTTGCC 8 425 1958 Antisense
CTCTTTGCCACTTCCATGAATTTGC 214 231 1974 Antisense
ATGAATTTGCTTACATCTCCTGATT 108 45 1989 Antisense
CATTTAACCCGTACTCTTCATTTAT 388 209 2038 Antisense
TATTGTTGCTGTTTTTCATCGACCC 501 665 2060 Antisense
TTTAGTCCCATGTTTCAGCATCCAA 593 673 2086 Antisense
AGCATCCAACATGTCTTTATTCCCC 385 77 2102 Antisense
TCTCCCGTAGCTCATTTCATTGTAA 276 611 2135 Antisense
AAATACTGATGAGACCCGCTGTAGC 110 117 2158 Antisense
TAGCCCTTTTTCATTCGTTTGTATT 701 651 2179 Antisense
CGTTTGTATTCATGGGCGAGGCGAT 395 241 2194 Antisense
Sequence Source GenBank