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[175826_s_at]

Description g5353962 /REP_DB=GenBank Identifier /GB=AF130406.1 /PROTEIN_GB=AAD42307.1 /CHR=X /FEA=Mapped Transcript /DEF=phophoinositide-dependent protein kinase 1a
Cluster 6grp_highest_10_10 > 01      6grp_high1_10_10 > 08      4grp_highest_8_8 > 52      4grp_high_8_8 > 16      6grp_highest_nonorm > 81      6grp_total > 19      4grp_total > 31      6grp_cgc > 10      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 196
1 2 3 4 range
A 215.8
(2.1)
P 		108.1
(-3)
P 		208.2
(1.8)
P 		233.7
(3)
P 		126
B 303.8
(3)
P 		188.9
(-1.9)
P 		163
(-3)
P 		224.5
(-0.4)
P 		141
C 219
(3)
P 		204.1
(1.9)
P 		139
(-3)
P 		145.2
(-2.5)
P 		80
avr 246.2
(3) 		167
(-3) 		170.1
(-2.8) 		201.1
(-0.4) 		79

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 164
1 2 3 4 5 6 range
A 383.1
(3)
P 		236.5
(-3)
P 		251.1
(-2.4)
P 		269.8
(-1.6)
P 		341.9
(1.3)
P 		322.2
(0.5)
P 		147
B 331
(0.5)
P 		302.1
(-0.6)
P 		400.7
(3)
P 		329.6
(0.4)
P 		322.7
(0.2)
P 		236.3
(-3)
P 		164
C 354.1
(3)
P 		284.6
(-3)
P 		315.9
(-0.3)
P 		329.6
(0.9)
P 		301.8
(-1.5)
P 		299.2
(-1.7)
P 		70
avr 356.1
(3) 		274.4
(-3) 		322.6
(0.5) 		309.7
(-0.4) 		322.1
(0.5) 		285.9
(-2.2) 		82
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 73
1 2 3 4 range
A 78.6
(3)
P 		23.5
(-3)
P 		54.4
(0.4)
P 		34.2
(-1.8)
P 		55
B 96.9
(3)
P 		46.6
(-2.1)
P 		37.8
(-3)
P 		47.8
(-2)
P 		59
C 72.5
(3)
P 		58.7
(1.3)
P 		48.4
(-0)
P 		24.5
(-3)
P 		48
avr 82.7
(3) 		42.9
(-2.1) 		46.9
(-1.6) 		35.5
(-3) 		47

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 77
1 2 3 4 5 6 range
A 70.6
(-1)
P 		84.1
(0.6)
P 		54.7
(-3)
P 		68.4
(-1.3)
P 		103.2
(3)
P 		78.5
(-0.1)
P 		49
B 115.2
(0.8)
P 		100.6
(-1.2)
P 		97.3
(-1.6)
P 		87.3
(-3)
P 		131.1
(3)
P 		88.1
(-2.9)
P 		44
C 131.3
(3)
P 		86.8
(-1.1)
P 		80.4
(-1.7)
P 		66.3
(-3)
P 		97.7
(-0.1)
P 		100.2
(0.1)
P 		65
avr 105.7
(2.2) 		90.5
(-0.3) 		77.5
(-2.4) 		74
(-3) 		110.7
(3) 		88.9
(-0.6) 		37
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175826_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20034
Sequence Type Exemplar sequence
Representative Public ID 5353962 NCBI
Target Description g5353962 /REP_DB=GenBank Identifier /GB=AF130406.1 /PROTEIN_GB=AAD42307.1 /CHR=X /FEA=Mapped Transcript /DEF=phophoinositide-dependent protein kinase 1a
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029572(11), NM_001029571(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029572 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
NM_001029571 NCBI Caenorhabditis elegans PDK-class protein kinase family member (pdk-1) (pdk-1) mRNA, complete cds. 11/11 None
H42K12.1b ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
H42K12.1a ENSEMBL cdna:known chromosome:CEL140:X:1321348:1327980:1 gene:H42K12.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:1321347-1327980(+) 100.0 100.0
Public Domain and Genome References
Ensembl H42K12.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK82905 Pdk-class protein kinase protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024743.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42307.1| phophoinositide-dependent protein kinase 1a [Caenorhabditis elegans] 0.0
blast AAK82904 Pdk-class protein kinase protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024742.1| PDK-class protein kinase family member (pdk-1) [Caenorhabditis elegans] gb|AAD42308.1| phophoinositide-dependent protein kinase 1b [Caenorhabditis elegans] 0.0
blast CAE63571 Hypothetical protein CBG08059 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-16
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 4.0E-16
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 6.0E-65
hanks 1.8.7 AGC group; AGC VIII Flowering plant PVPK1 homologs; PsPK5 1.0E-65
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.2E-68
Pfam IPR000719 EMBL-EBI Protein kinase 2.2E-68
Sequence
Target Sequence 
>C. ELEGANS:175826_S_AT
agaagtcgccttgaaggctcacctcccttctactccccacaaaatcaccatcaaacaaat
cacacttttgtatcattttgcgtccttacatctcctttcattcctcactttggttttttc
caactgtcattaaatatctgtaggtactgagatatccccccacgtcacacttgctctcat
cacacacaggctgtcatagttgtttcacacctttttacacactttttctccattccaact
gctagaatgcacactgtagacgtagacgtctttgtcttgtactattttgttttttttttg
cttaaattgggtttaatcctttttcaaaaatttcacaatttttatacactgcgtacgtag
aaccacactctgttttttttgtcatttttttttgtttcccttacaatgatctgtttttct
ttttttttctccctgtcgattcgtaccatttgttagctg

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AGAAGTCGCCTTGAAGGCTCACCTC 208 73 1920 Antisense
TGTATCATTTTGCGTCCTTACATCT 347 561 1988 Antisense
TCATTCCTCACTTTGGTTTTTTCCA 691 617 2017 Antisense
CTGTAGGTACTGAGATATCCCCCCA 246 239 2057 Antisense
CTCTCATCACACACAGGCTGTCATA 231 231 2093 Antisense
GTTGTTTCACACCTTTTTACACACT 600 437 2118 Antisense
TTTTTCTCCATTCCAACTGCTAGAA 381 675 2142 Antisense
AATGCACACTGTAGACGTAGACGTC 599 165 2165 Antisense
GACGTCTTTGTCTTGTACTATTTTG 676 377 2184 Antisense
GTACGTAGAACCACACTCTGTTTTT 352 459 2272 Antisense
GTCGATTCGTACCATTTGTTAGCTG 440 473 2354 Antisense
Sequence Source GenBank