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[175823_s_at]

Description g2618976 /REP_DB=GenBank Identifier /GB=AF020342.1 /PROTEIN_GB=AAB84390.1 /CHR=1 /FEA=Mapped Transcript /DEF=fork head-related transcription factor DAF-16a1
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 57      4grp_highest_8_8 > 31      4grp_high_8_8 > 05      6grp_highest_nonorm > 96      6grp_total > 59      4grp_total > 03      6grp_highest_sub > Cu      6grp_cgc > 39      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L4      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 2165
1 2 3 4 range
A 949.3
(-2.1)
P 		808.6
(-2.5)
P 		645.4
(-3)
P 		2606.7
(3)
P 		1961
B 1078.2
(-1.5)
P 		553.5
(-3)
P 		550.5
(-3)
P 		2715.9
(3)
P 		2165
C 803.4
(-2.3)
P 		710.2
(-2.6)
P 		560
(-3)
P 		2652.7
(3)
P 		2093
avr 943.6
(-2) 		690.8
(-2.7) 		585.3
(-3) 		2658.4
(3) 		2073

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1066
1 2 3 4 5 6 range
A 1199.4
(1)
P 		728.1
(-1.6)
P 		485.4
(-3)
P 		656.2
(-2)
P 		893.2
(-0.7)
P 		1551.3
(3)
P 		1066
B 913.6
(0.6)
P 		699.9
(-1.2)
P 		490.4
(-3)
P 		753
(-0.8)
P 		665.1
(-1.5)
P 		1200.2
(3)
P 		710
C 989.4
(1.9)
P 		709.7
(-3)
P 		711.5
(-3)
P 		772.2
(-1.9)
P 		877.5
(-0.1)
P 		1054.7
(3)
P 		345
avr 1034.1
(1) 		712.6
(-1.7) 		562.4
(-3) 		727.1
(-1.6) 		811.9
(-0.9) 		1268.7
(3) 		706
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 451
1 2 3 4 range
A 345.7
(2)
P 		175.5
(-2.8)
P 		168.7
(-3)
P 		381.6
(3)
P 		213
B 343.8
(-0.1)
P 		136.7
(-2.9)
P 		127.6
(-3)
P 		578.8
(3)
P 		451
C 266
(-1.3)
P 		204.2
(-2.8)
P 		195.2
(-3)
P 		447.3
(3)
P 		252
avr 318.5
(0) 		172.1
(-2.8) 		163.8
(-3) 		469.2
(3) 		305

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 342
1 2 3 4 5 6 range
A 221.1
(-0.5)
P 		259
(0.4)
P 		105.7
(-3)
P 		166.4
(-1.7)
P 		269.5
(0.6)
P 		377.7
(3)
P 		272
B 317.9
(0.6)
P 		233
(-0.9)
P 		119.1
(-3)
P 		199.5
(-1.5)
P 		270.2
(-0.2)
P 		447.5
(3)
P 		328
C 366.7
(3)
P 		216.6
(-1.3)
P 		181.1
(-2.3)
P 		155.3
(-3)
P 		283.9
(0.6)
P 		353.1
(2.6)
P 		211
avr 301.9
(0.9) 		236.2
(-0.6) 		135.3
(-3) 		173.7
(-2.1) 		274.5
(0.2) 		392.8
(3) 		258
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175823_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19642
Sequence Type Exemplar sequence
Representative Public ID 2618976 NCBI
Target Description g2618976 /REP_DB=GenBank Identifier /GB=AF020342.1 /PROTEIN_GB=AAB84390.1 /CHR=1 /FEA=Mapped Transcript /DEF=fork head-related transcription factor DAF-16a1
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001026427(11), NM_001026424(11), NM_001026423(11), NM_001026422(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001026427 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026424 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026423 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
NM_001026422 NCBI Caenorhabditis elegans abnormal DAuer Formation family member (daf-16) (daf-16) mRNA, complete cds. 11/11 None
R13H8.1b ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784824:1 gene:R13H8.1 11/11 A 
R13H8.1c ENSEMBL cdna:known chromosome:CEL140:I:10770891:10784821:1 gene:R13H8.1 11/11 A 
R13H8.1a ENSEMBL cdna:known chromosome:CEL140:I:10779578:10784583:1 gene:R13H8.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
172981 6 4 NM_001026422 175823_s_at (11)
NM_001026423 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026424 175678_at (11), 175823_s_at (11), 181992_s_at (11)
NM_001026427 175678_at (11), 175823_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:10770856-10784331(+) 100.0 100.0
Public Domain and Genome References
Ensembl R13H8.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast NP_001021598 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021596 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] 0.0
blast NP_001021595 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82919.1| Abnormal dauer formation protein 16, isoform c [Caenorhabditis elegans] gb|AAC47803.1| DAF-16 [Caenorhabditis elegans] gb|AAB84390.1| fork head-related transcription factor DAF-16a1 [Caenorhabditis elegans] 0.0
blast NP_001021594 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82918.1| Abnormal dauer formation protein 16, isoform b [Caenorhabditis elegans] gb|AAB84391.1| fork head-related transcription factor DAF-16a2 [Caenorhabditis elegans] 0.0
blast NP_001021593 abnormal DAuer Formation family member (daf-16) [Caenorhabditis elegans] gb|AAK82917.1| Abnormal dauer formation protein 16, isoform a [Caenorhabditis elegans] gb|AAB84392.1| fork head-related transcription factor DAF-16b [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 8.968310171678829E-44
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 2.00385680398449E-43
scop a.4.5.Forkhead DNA-binding domain All alpha proteins; DNA/RNA-binding 3-helical bundle; "Winged helix" DNA-binding domain; Forkhead DNA-binding domain 9.99999991097579E-38
Pfam IPR001766 EMBL-EBI Fork head transcription factor 5.4E-22
Pfam IPR001766 EMBL-EBI Fork head transcription factor 8.7E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 6.2E-23
Pfam IPR001766 EMBL-EBI Fork head transcription factor 9.1E-17
Sequence
Target Sequence 
>C. ELEGANS:175823_S_AT
ctattttctattttctgtcgtttgttcccctttctctcgtctaattccaacacattcatc
ccagtgacgtcgtgtaataataatataaaatacctcttctctctttcttcccctaatgcg
aaatatcgaaaaaccgttgattattacctcttttttcttgttttttttttctctctctct
ctcccgtcatccaggttcttcactctttaaatgctacctctatcccatctttttcgctgt
aaatttgtttcgcaatcaaaactgctaaaacacattccccaatctgtcttttttaattga
atttttcaaaaaatttgatttcttgatttctcttgtaattctttaattttcctctttttt
ttccccctggtagcaaatgtctagcgattctctttctttttttgtttaactttcacatct
ggccgattcgaatcctccgtatacacacacacatagtaatctacctccaaaattttactg
aaagatgtgatcccctctct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTATTTTCTATTTTCTGTCGTTTGT 129 225 2032 Antisense
AACACATTCATCCCAGTGACGTCGT 496 135 2080 Antisense
ATATAAAATACCTCTTCTCTCTTTC 374 7 2114 Antisense
TGATTATTACCTCTTTTTTCTTGTT 27 565 2169 Antisense
CATCCAGGTTCTTCACTCTTTAAAT 253 211 2219 Antisense
TATCCCATCTTTTTCGCTGTAAATT 288 659 2252 Antisense
AAAACACATTCCCCAATCTGTCTTT 57 131 2298 Antisense
TTTTCCCCCTGGTAGCAAATGTCTA 28 661 2390 Antisense
AAATGTCTAGCGATTCTCTTTCTTT 87 121 2406 Antisense
TCACATCTGGCCGATTCGAATCCTC 159 629 2444 Antisense
TACTGAAAGATGTGATCCCCTCTCT 567 645 2507 Antisense
Sequence Source GenBank