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[175822_at]

Description g2547168 /REP_DB=GenBank Identifier /GB=AF023602.1 /PROTEIN_GB=AAC47755.1 /CHR=4 /FEA=Mapped Transcript /DEF=putative L-type calcium channel alpha 1 subunit
Cluster 6grp_highest_10_10 > 42      6grp_high1_10_10 > 14      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 30      6grp_total > 15      4grp_total > 38      6grp_highest_sub > 02      6grp_cgc > 32      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S2      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 909
1 2 3 4 range
A 1271.3
(3)
P 		502.4
(-2.2)
P 		398.6
(-2.9)
P 		390.3
(-3)
P 		881
B 1159.6
(3)
P 		535.9
(-1.7)
P 		362.3
(-3)
P 		612.5
(-1.1)
P 		797
C 1091.9
(3)
P 		694.9
(-0.6)
P 		424.2
(-3)
P 		569
(-1.7)
P 		668
avr 1174.3
(3) 		577.7
(-1.6) 		395
(-3) 		523.9
(-2) 		779

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1005
1 2 3 4 5 6 range
A 1368.1
(3)
P 		1027.7
(1)
P 		671.4
(-1.2)
P 		395.8
(-2.8)
P 		814.5
(-0.3)
P 		363.1
(-3)
P 		1005
B 1136.2
(2.6)
P 		1196.4
(3)
P 		900.9
(0.9)
P 		848.3
(0.5)
P 		548.5
(-1.7)
P 		371.5
(-3)
P 		825
C 902.8
(0)
P 		1356.9
(3)
P 		670.8
(-1.5)
P 		696.4
(-1.3)
P 		439
(-3)
P 		519.7
(-2.5)
P 		918
avr 1135.7
(2.6) 		1193.7
(3) 		747.7
(-0.5) 		646.8
(-1.2) 		600.7
(-1.6) 		418.1
(-3) 		776
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 406
1 2 3 4 range
A 462.9
(3)
P 		109.1
(-2.2)
P 		104.2
(-2.3)
P 		57.1
(-3)
P 		406
B 369.8
(3)
P 		132.4
(-2)
P 		84
(-3)
P 		130.5
(-2)
P 		286
C 361.5
(3)
P 		199.8
(-0.7)
P 		147.8
(-1.8)
P 		95.9
(-3)
P 		266
avr 398.1
(3) 		147.1
(-2) 		112
(-2.7) 		94.5
(-3) 		304

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 326
1 2 3 4 5 6 range
A 252.2
(0.5)
P 		365.5
(3)
P 		146.2
(-1.7)
P 		100.4
(-2.7)
P 		245.8
(0.4)
P 		88.4
(-3)
P 		277
B 395.3
(2.9)
P 		398.2
(3)
P 		218.8
(-1.1)
P 		224.7
(-1)
P 		222.8
(-1.1)
P 		138.5
(-3)
P 		260
C 334.6
(1.3)
P 		414
(3)
P 		170.7
(-2.3)
P 		140
(-3)
P 		142
(-3)
P 		174
(-2.3)
P 		274
avr 327.4
(1.5) 		392.6
(3) 		178.6
(-2) 		155
(-2.5) 		203.5
(-1.4) 		133.6
(-3) 		259
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175822_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19869
Sequence Type Exemplar sequence
Representative Public ID 2547168 NCBI
Target Description g2547168 /REP_DB=GenBank Identifier /GB=AF023602.1 /PROTEIN_GB=AAC47755.1 /CHR=4 /FEA=Mapped Transcript /DEF=putative L-type calcium channel alpha 1 subunit
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_171379(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_171379 NCBI Caenorhabditis elegans EGg Laying defective family member (egl-19) (egl-19) mRNA, complete cds. 11/11 A 
C48A7.1a ENSEMBL cdna:known chromosome:CEL140:IV:7405533:7418717:1 gene:C48A7.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177513 2 1 NM_171379 175822_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:7405538-7418723(+) 97.65 100.0
Public Domain and Genome References
Ensembl C48A7.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAB03158 Egg laying defective protein 19, isoform a [Caenorhabditis elegans] ref|NP_741442.1| EGg Laying defective family member (egl-19) [Caenorhabditis elegans] gb|AAC47755.1| putative L-type calcium channel alpha 1 subunit [Caenorhabditis elegans] 0.0
blast CAE61874 Hypothetical protein CBG05858 [Caenorhabditis briggsae] 0.0
blast AAM75372 Egg laying defective protein 19, isoform b [Caenorhabditis elegans] ref|NP_001023079.1| EGg Laying defective family member (egl-19) [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR005821 EMBL-EBI Ion transport protein 3.3E-73
Pfam IPR005821 EMBL-EBI Ion transport protein 2.5E-53
Pfam IPR005821 EMBL-EBI Ion transport protein 1.0E-65
Pfam IPR005821 EMBL-EBI Ion transport protein 6.7E-67
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_741442.1 18 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1152-1171,1246-1268,1341-1363
C48A7.1a 18 89-108,128-150,157-176,221-243,313-335,345-367,475-492,507-529,600-622,672-694,794-816,836-858,927-949,1043-1065,1110-1132,1152-1171,1246-1268,1341-1363
Sequence
Target Sequence 
>C. ELEGANS:175822_AT
aaaccacctcattcaatttgtacgagtttagaattcatctttttcttctttttccctcaa
tcatcccaactcgaatcttccctgtagatgatcccccatcccatcttgtctgtgtactgg
ttttttttgtttactgtataattcatttaatcttaccaaaacctgctcccatttcaaaaa
tgctcaatatctctgtctagttggctgatggatatttggctcacagagttcgctttcata
actcaacttgcatattgtccagaagtcctctagttatcattttattcatttttttcacaa
aaaaccaaatctgatcaaaagtggcctttctgcgatttcacttagaatcgttcattactc
ttatttgaagtttaaatgtatctttctttcccctttttctttcaattaatatatatctct
attttcccatatttctaagtcaaaaacttcctcttcattttctcatccctcccatccttt
acgcagtatctgcttatccttttaatagttcatttttctcatttattttgattttcaaca
acaattcgtggtgcttttcttgttttat

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
AAACCACCTCATTCAATTTGTACGA 539 125 5593 Antisense
CGAATCTTCCCTGTAGATGATCCCC 132 253 5664 Antisense
CCATCCCATCTTGTCTGTGTACTGG 367 271 5688 Antisense
CAAAACCTGCTCCCATTTCAAAAAT 94 189 5749 Antisense
AAATGCTCAATATCTCTGTCTAGTT 271 119 5770 Antisense
ATTTGGCTCACAGAGTTCGCTTTCA 249 19 5806 Antisense
TTCGCTTTCATAACTCAACTTGCAT 377 697 5821 Antisense
AAAAGTGGCCTTTCTGCGATTTCAC 173 133 5909 Antisense
CAAAAACTTCCTCTTCATTTTCTCA 92 189 6033 Antisense
CCCATCCTTTACGCAGTATCTGCTT 581 257 6063 Antisense
ATTCGTGGTGCTTTTCTTGTTTTAT 524 9 6136 Antisense
Sequence Source GenBank