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[175818_s_at] alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase

Description g3420839 /REP_DB=GenBank Identifier /GB=AF082010.1 /PROTEIN_GB=AAD03022.1 /CHR=X /FEA=Mapped Transcript /DEF=UDP-N-acetylglucosamine:a-3-D-mannosideb-1,2-N-acetylglucosaminyltransferase I
Cluster 6grp_highest_10_10 > 10      6grp_high1_10_10 > 23      4grp_highest_8_8 > 36      4grp_high_8_8 > 08      6grp_highest_nonorm > 62      6grp_total > 13      4grp_total > 15      6grp_cgc > 40      6grp_high1_sub > 02      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 345
1 2 3 4 range
A 631.6
(3)
P 		333.8
(-3)
P 		505.1
(0.5)
P 		620.9
(2.8)
P 		298
B 646.2
(3)
P 		431
(-2)
P 		389.7
(-3)
P 		502.5
(-0.4)
P 		257
C 679
(3)
P 		471.1
(-3)
P 		631.7
(1.6)
P 		536.3
(-1.1)
P 		208
avr 652.3
(3) 		412
(-3) 		508.8
(-0.6) 		553.2
(0.5) 		240

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 299
1 2 3 4 5 6 range
A 520.3
(2.6)
P 		444.1
(0.1)
P 		532.4
(3)
P 		487.8
(1.5)
P 		498.4
(1.9)
P 		351.1
(-3)
P 		181
B 649.5
(3)
P 		596
(1.9)
P 		364.5
(-3)
P 		565.6
(1.2)
P 		409.8
(-2)
P 		492.2
(-0.3)
P 		285
C 485.5
(-0.1)
P 		633.9
(3)
P 		520
(0.6)
P 		583.4
(1.9)
P 		350.7
(-3)
P 		360.8
(-2.8)
P 		283
avr 551.8
(2.8) 		558
(3) 		472.3
(-0.3) 		545.6
(2.5) 		419.6
(-2.3) 		401.4
(-3) 		157
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 157
1 2 3 4 range
A 229.9
(3)
P 		72.5
(-3)
P 		132
(-0.7)
P 		90.9
(-2.3)
P 		157
B 206.1
(3)
P 		106.5
(-2.2)
P 		90.3
(-3)
P 		107.1
(-2.1)
P 		116
C 224.8
(3)
P 		135.4
(-1)
P 		220.2
(2.8)
P 		90.4
(-3)
P 		134
avr 220.3
(3) 		104.8
(-2.6) 		147.5
(-0.5) 		96.1
(-3) 		124

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 141
1 2 3 4 5 6 range
A 95.9
(-2.1)
P 		157.9
(3)
P 		116
(-0.5)
P 		123.7
(0.2)
P 		150.4
(2.4)
P 		85.5
(-3)
P 		72
B 226
(3)
P 		198.4
(1.8)
P 		88.5
(-3)
P 		149.8
(-0.3)
P 		166.5
(0.4)
P 		183.5
(1.1)
P 		138
C 180
(2)
P 		193.4
(3)
P 		132.4
(-1.6)
P 		117.3
(-2.7)
P 		113.5
(-3)
P 		120.8
(-2.5)
P 		80
avr 167.3
(1.7) 		183.2
(3) 		112.3
(-3) 		130.3
(-1.5) 		143.5
(-0.4) 		129.9
(-1.5) 		71
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175818_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19961
Sequence Type Exemplar sequence
Representative Public ID 3420839 NCBI
Target Description g3420839 /REP_DB=GenBank Identifier /GB=AF082010.1 /PROTEIN_GB=AAD03022.1 /CHR=X /FEA=Mapped Transcript /DEF=UDP-N-acetylglucosamine:a-3-D-mannosideb-1,2-N-acetylglucosaminyltransferase I
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_077165(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_077165 NCBI Caenorhabditis elegans GLYcosylation related family member (gly-13) (gly-13) mRNA, complete cds. 11/11 A 
B0416.6.1 ENSEMBL cdna:known chromosome:CEL140:X:9293605:9296554:-1 gene:B0416.6 11/11 None
B0416.6.2 ENSEMBL cdna:known chromosome:CEL140:X:9293605:9296554:-1 gene:B0416.6 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9293591-9296552(-) 100.0 100.0
Public Domain and Genome References
Gene Title alpha-1,3-mannosyl-glycoprotein beta-1,2-n-acetyglucosaminyltransferase
Gene Symbol gly-13
Ensembl B0416.6
Entrez Gene 181160 Entrez gene
SwissProt Q11068 EMBL-EBI
WormBase CE26434 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6487 protein amino acid N-linked glycosylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
139 Golgi membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3827 alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAG38885 Glycosylation related protein 13 [Caenorhabditis elegans] ref|NP_509566.1| GLYcosylation related family member (gly-13) [Caenorhabditis elegans] sp|Q11068|MGAT1_CAEEL Putative alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase (N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I) (GNT-I) (GlcNAc-T I) gb|AAD03022.1| UDP-N-acetylglucosamine:a-3-D-mannoside b-1,2-N-acetylglucosaminyltransferase I [Caenorhabditis elegans] 0.0
blast E89606 protein B0416.6 [imported] - Caenorhabditis elegans 0.0
blast CAE69842 Hypothetical protein CBG16168 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004139 EMBL-EBI Glycosyl transferase, family 13 1.0E-126
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_509566.1 1 7-22
B0416.6.1 1 7-22
B0416.6.2 1 7-22
Sequence
Target Sequence 
>C. ELEGANS:175818_S_AT
ggagagtagttttgtggctttgataatgtttaaactttatatttcacgggtttcattctt
accgaatctagtcgactctggcctgcattcctattattttgttcaactctttccagtgaa
tctgacatttgcagctagtaatttgatgtgaaaattattaccttgtttcgagatgtgatt
gccctacgaaattcatatcttttctcagcaattgcacgttttatttgctacgttggttgt
tattttaggggtattgtaattgattatcaatctttttctttccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
GGAGAGTAGTTTTGTGGCTTTGATA 102 519 1384 Antisense
TTCACGGGTTTCATTCTTACCGAAT 479 681 1426 Antisense
TACCGAATCTAGTCGACTCTGGCCT 544 649 1443 Antisense
TCTGGCCTGCATTCCTATTATTTTG 416 607 1460 Antisense
TATTTTGTTCAACTCTTTCCAGTGA 666 661 1478 Antisense
GTGAATCTGACATTTGCAGCTAGTA 658 481 1499 Antisense
TGTTTCGAGATGTGATTGCCCTACG 688 559 1547 Antisense
GATTGCCCTACGAAATTCATATCTT 664 433 1560 Antisense
TCATATCTTTTCTCAGCAATTGCAC 643 619 1576 Antisense
TATTTGCTACGTTGGTTGTTATTTT 192 661 1605 Antisense
TGATTATCAATCTTTTTCTTTCCAA 604 563 1644 Antisense
Sequence Source GenBank