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[175814_at]

Description Y34F4.B /REP_DB=WormBase Gene ID /CHR=3 /FEA=Sanger Annotation
Cluster 6grp_total > 25      4grp_total > 08      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 74
1 2 3 4 range
A 41.8
(1.4)
A 		3.7
(-3)
A 		25.5
(-0.5)
A 		56
(3)
A 		52
B 2.4
(-3)
A 		12.1
(-1)
A 		31.3
(3)
A 		9.9
(-1.4)
A 		29
C 12.5
(-3)
A 		48.1
(0.4)
A 		29
(-1.4)
A 		76.2
(3)
A 		64
avr 18.9
(-3) 		21.3
(-2.5) 		28.6
(-1) 		47.4
(3) 		29

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 44
1 2 3 4 5 6 range
A 45.4
(3)
A 		22.6
(-0.1)
A 		33.6
(1.4)
A 		1.7
(-3)
A 		19.7
(-0.5)
A 		3.8
(-2.7)
A 		44
B 10.7
(-2.1)
A 		29.3
(3)
A 		23.1
(1.3)
A 		19.3
(0.2)
A 		20.1
(0.5)
A 		7.6
(-3)
A 		22
C 30.9
(2.1)
A 		35.9
(3)
A 		2.4
(-2.9)
A 		6
(-2.3)
A 		2.1
(-3)
A 		27.4
(1.5)
A 		34
avr 29
(2.9) 		29.3
(3) 		19.7
(0.2) 		9
(-3) 		14
(-1.5) 		12.9
(-1.8) 		20
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 14
1 2 3 4 range
A 15.2
(3)
A 		0.8
(-3)
A 		6.7
(-0.5)
A 		8.2
(0.1)
A 		14
B 0.8
(-3)
A 		3
(-0.9)
A 		7.2
(3)
A 		2.1
(-1.8)
A 		6
C 4.1
(-3)
A 		13.8
(3)
A 		10.1
(0.7)
A 		12.8
(2.4)
A 		10
avr 6.7
(-0.7) 		5.9
(-3) 		8
(3) 		7.7
(2.1) 		2

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 11
1 2 3 4 5 6 range
A 8.4
(3)
A 		8.1
(2.8)
A 		7.3
(2.2)
A 		0.4
(-3)
A 		6
(1.2)
A 		0.9
(-2.6)
A 		8
B 3.7
(-2.2)
A 		9.8
(3)
A 		5.6
(-0.6)
A 		5.1
(-1)
A 		8.2
(1.6)
A 		2.8
(-3)
A 		7
C 11.4
(3)
A 		11
(2.8)
A 		0.6
(-3)
A 		1.2
(-2.7)
A 		0.7
(-2.9)
A 		9.2
(1.8)
A 		11
avr 7.8
(1.5) 		9.6
(3) 		4.5
(-1.1) 		2.2
(-3) 		5
(-0.7) 		4.3
(-1.3) 		7
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175814_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.18979
Sequence Type Exemplar sequence
Representative Public ID Y34F4.B NCBI
Target Description Y34F4.B /REP_DB=WormBase Gene ID /CHR=3 /FEA=Sanger Annotation
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_064913(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_064913 NCBI Caenorhabditis elegans Y34F4.3 (Y34F4.3) mRNA, complete cds. 11/11 A 
GENEFINDER00000027781 ENSEMBL cdna:GeneFinder chromosome:CEL140:III:1015014:1016278:-1 11/11 None
SNAP00000027777 ENSEMBL cdna:SNAP chromosome:CEL140:III:1015671:1023485:-1 11/11 None
Y34F4.3 ENSEMBL cdna:known chromosome:CEL140:III:1015671:1016084:-1 gene:Y34F4.3 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000015408 1/11 Cross Hyb Matching Probes None
B0391.8 1/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1015670-1016084(-) 89.34 100.0
Public Domain and Genome References
Gene Title
Ensembl Y34F4.3
Entrez Gene 189592 Entrez gene
SwissProt Q9GR67 EMBL-EBI
WormBase CE26639 Wormbase
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAG37940 Hypothetical protein Y34F4.3 [Caenorhabditis elegans] ref|NP_497314.1| Y34F4.3 [Caenorhabditis elegans] 2.0E-68
blast AAG37940 Hypothetical protein Y34F4.3 [Caenorhabditis elegans] ref|NP_497314.1| Y34F4.3 [Caenorhabditis elegans] 4.0E-68
blast AAG37940 Hypothetical protein Y34F4.3 [Caenorhabditis elegans] ref|NP_497314.1| Y34F4.3 [Caenorhabditis elegans] 2.0E-67
blast AAA62540 Hypothetical protein K10D2.2 [Caenorhabditis elegans] ref|NP_498100.1| K10D2.2 [Caenorhabditis elegans] 2.0E-42
blast AAA62540 Hypothetical protein K10D2.2 [Caenorhabditis elegans] ref|NP_498100.1| K10D2.2 [Caenorhabditis elegans] 8.0E-42
blast AAA62540 Hypothetical protein K10D2.2 [Caenorhabditis elegans] ref|NP_498100.1| K10D2.2 [Caenorhabditis elegans] 5.0E-41
blast CAE60032 Hypothetical protein CBG03540 [Caenorhabditis briggsae] 1.0E-40
blast CAE60032 Hypothetical protein CBG03540 [Caenorhabditis briggsae] 5.0E-39
Protein Domains
Database ID Description E-Value
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 7.9E-17
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 7.9E-17
Pfam IPR002058 EMBL-EBI PAP/25A-associated domain 7.9E-17
Sequence
Target Sequence 
>C. ELEGANS:175814_AT
atgctctcgtttcgatgccagagtcgtgcttctgtcgttgatttgttcgttggattcttg
gattcttggattcttggattcttggattgctatgccacatactttgactactccacaaat
tttatccagatggtgtccaagaaattggagtacaaaccagatcgctggtgcaagtatcca
atgtgtattgctgatccgttcaagacggatcataacttggaacaaggagttgtgctgcaa
atgttcgaattcattcgatcttgtatggaacactcgatggaagtattcacggattgccgc
attcgttcggattgtcactcgagatacgag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGCTCTCGTTTCGATGCCAGAGTC 223 41 16 Antisense
CGATGCCAGAGTCGTGCTTCTGTCG 301 249 28 Antisense
ATTGGAGTACAAACCAGATCGCTGG 329 3 159 Antisense
GTACAAACCAGATCGCTGGTGCAAG 667 459 165 Antisense
AGACGGATCATAACTTGGAACAAGG 123 65 218 Antisense
GGAGTTGTGCTGCAAATGTTCGAAT 183 519 241 Antisense
CATTCGATCTTGTATGGAACACTCG 693 215 267 Antisense
GGAACACTCGATGGAAGTATTCACG 227 529 282 Antisense
GTATTCACGGATTGCCGCATTCGTT 129 453 298 Antisense
GATTGCCGCATTCGTTCGGATTGTC 674 433 307 Antisense
TTCGGATTGTCACTCGAGATACGAG 326 695 321 Antisense
Sequence Source Affymetrix Proprietary Database