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[175809_at]

Description g3694832 /REP_DB=GenBank Identifier /GB=AF072381.1 /PROTEIN_GB=AAC62468.1 /CHR=X /FEA=Mapped Transcript /DEF=Akt-PKB serine-threonine kinase
Cluster 6grp_highest_10_10 > 93      6grp_high1_10_10 > 48      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 85      6grp_total > 48      4grp_total > 15      6grp_highest_sub > Cu      6grp_cgc > 18      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 908
1 2 3 4 range
A 1061
(3)
P 		442.8
(-3)
P 		829.1
(0.7)
P 		855
(1)
P 		618
B 1351.2
(3)
P 		599.9
(-2.2)
P 		480.5
(-3)
P 		1207.4
(2)
P 		871
C 833.7
(-0.1)
P 		620.4
(-3)
P 		794.4
(-0.6)
P 		1061.4
(3)
P 		441
avr 1082
(3) 		554.4
(-3) 		701.3
(-1.3) 		1041.3
(2.5) 		528

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 848
1 2 3 4 5 6 range
A 967.1
(2.6)
P 		693.4
(-1.6)
P 		603.5
(-3)
P 		640.2
(-2.4)
P 		710.9
(-1.3)
P 		993.1
(3)
P 		390
B 941.9
(1.6)
P 		665.5
(-0.4)
P 		296
(-3)
P 		739.4
(0.1)
P 		692.1
(-0.2)
P 		1144
(3)
P 		848
C 970.7
(3)
P 		686.1
(-1.5)
P 		588.4
(-3)
P 		617.3
(-2.5)
P 		642.1
(-2.2)
P 		895.8
(1.8)
P 		382
avr 959.9
(2.4) 		681.7
(-0.8) 		496
(-3) 		665.6
(-1) 		681.7
(-0.8) 		1011
(3) 		515
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 335
1 2 3 4 range
A 386.3
(3)
P 		96.1
(-3)
P 		216.7
(-0.5)
P 		125.2
(-2.4)
P 		290
B 430.9
(3)
P 		148.2
(-2.3)
P 		111.3
(-3)
P 		257.3
(-0.3)
P 		320
C 276
(2.9)
P 		178.4
(-3)
P 		276.9
(3)
P 		179
(-3)
P 		99
avr 364.4
(3) 		140.9
(-3) 		201.6
(-1.4) 		187.2
(-1.8) 		224

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 355
1 2 3 4 5 6 range
A 178.3
(-0.6)
P 		246.6
(3)
P 		131.4
(-3)
P 		162.4
(-1.4)
P 		214.5
(1.3)
P 		241.8
(2.8)
P 		115
B 327.7
(1.3)
P 		221.5
(-0.5)
P 		71.9
(-3)
P 		195.9
(-0.9)
P 		281.2
(0.5)
P 		426.6
(3)
P 		355
C 359.8
(3)
P 		209.3
(-0.8)
P 		149.8
(-2.3)
P 		124.1
(-3)
P 		207.8
(-0.9)
P 		299.9
(1.5)
P 		236
avr 288.6
(2) 		225.8
(0.2) 		117.7
(-3) 		160.8
(-1.7) 		234.5
(0.4) 		322.8
(3) 		205
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175809_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.20012
Sequence Type Exemplar sequence
Representative Public ID 3694832 NCBI
Target Description g3694832 /REP_DB=GenBank Identifier /GB=AF072381.1 /PROTEIN_GB=AAC62468.1 /CHR=X /FEA=Mapped Transcript /DEF=Akt-PKB serine-threonine kinase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029441(11), NM_077956(9)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029441 NCBI Caenorhabditis elegans AKT kinase family member (akt-2) (akt-2) mRNA, complete cds. 11/11 None
NM_077956 NCBI Caenorhabditis elegans AKT kinase family member (akt-2) (akt-2) mRNA, complete cds. 9/11 A 
F28H6.1b.1 ENSEMBL cdna:known chromosome:CEL140:X:14147618:14151990:1 gene:F28H6.1 11/11 None
F28H6.1b.2 ENSEMBL cdna:known chromosome:CEL140:X:14147620:14151990:1 gene:F28H6.1 11/11 None
F28H6.1a ENSEMBL cdna:known chromosome:CEL140:X:14147627:14151862:1 gene:F28H6.1 9/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:14147619-14151990(+) 100.0 100.0
Public Domain and Genome References
Ensembl F28H6.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAC70087 Hypothetical protein F28H6.1b [Caenorhabditis elegans] emb|CAD21654.1| Hypothetical protein F28H6.1b [Caenorhabditis elegans] ref|NP_001024612.1| AKT kinase family member (akt-2) [Caenorhabditis elegans] gb|AAC62468.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAA20936 Hypothetical protein F28H6.1a [Caenorhabditis elegans] emb|CAB07403.1| Hypothetical protein F28H6.1a [Caenorhabditis elegans] ref|NP_510357.3| AKT kinase family member (akt-2) [Caenorhabditis elegans] 0.0
blast CAA98240 Hypothetical protein C12D8.10a [Caenorhabditis elegans] ref|NP_001023645.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62466.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 7.0E-67
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 6.0E-99
hanks 1.3.1 AGC group; AGC III RAC(Akt); Akt1/Raca 3.0E-99
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 3.3E-87
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.7E-21
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 3.5E-12
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.0E-19
Sequence
Target Sequence 
>C. ELEGANS:175809_AT
tgtacgtttttttctcattcactcaattttgtcaacatttcattgtctcctttgaaaact
tgttatctctttcatgaacttaacaaaatctctttcaaacatttcaacctttccaaaaac
tgaaactttgcgtagagcacatttcgtgttgctcacatcctctgggctaatttccattcc
tttcctgttaaaactccccatttcgcatcacttttttgacaatgctcttttatcatgttt
ttctgtagtacttgattcatatacccatctctcgggtatatttaaaacttttaaaacaca
gttccttttcttgataacatctttttcttgatattttgtcgccgacattttttctcggga
atgaacctttctcaca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TGTACGTTTTTTTCTCATTCACTCA 100 561 1913 Antisense
TGTCAACATTTCATTGTCTCCTTTG 505 559 1942 Antisense
TCTTTCAAACATTTCAACCTTTCCA 407 613 2003 Antisense
CATCCTCTGGGCTAATTTCCATTCC 385 211 2068 Antisense
CCCATTTCGCATCACTTTTTTGACA 519 259 2109 Antisense
TGCTCTTTTATCATGTTTTTCTGTA 423 579 2135 Antisense
TACTTGATTCATATACCCATCTCTC 449 645 2161 Antisense
AACACAGTTCCTTTTCTTGATAACA 378 135 2207 Antisense
TTTTTCTTGATATTTTGTCGCCGAC 561 675 2234 Antisense
TGTCGCCGACATTTTTTCTCGGGAA 707 557 2249 Antisense
TTCTCGGGAATGAACCTTTCTCACA 554 691 2264 Antisense
Sequence Source GenBank