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[175808_s_at]

Description g3694830 /REP_DB=GenBank Identifier /GB=AF072380.1 /PROTEIN_GB=AAC62467.1 /CHR=5 /FEA=Mapped Transcript /DEF=Akt-PKB serine-threonine kinase
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 78      4grp_highest_8_8 > 45      4grp_high_8_8 > 01      6grp_highest_nonorm > 68      6grp_total > 69      4grp_total > 15      6grp_highest_sub > Cu      6grp_cgc > 29      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1440
1 2 3 4 range
A 1995
(3)
P 		1171.5
(-3)
P 		1431.9
(-1.1)
P 		1524.8
(-0.4)
P 		824
B 2158.7
(0.7)
P 		1523.2
(-2.5)
P 		1426.1
(-3)
P 		2611.9
(3)
P 		1186
C 2253.2
(3)
P 		1695.2
(-3)
P 		1774.7
(-2.1)
P 		2223.7
(2.7)
P 		558
avr 2135.6
(3) 		1463.3
(-3) 		1544.2
(-2.3) 		2120.1
(2.9) 		672

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2006
1 2 3 4 5 6 range
A 2142.9
(-0.7)
P 		2025.1
(-1.1)
P 		1520.8
(-3)
P 		1748.8
(-2.1)
P 		2457.4
(0.5)
P 		3123.9
(3)
P 		1603
B 2083.8
(-0.3)
P 		1950.2
(-0.7)
P 		1201.4
(-3)
P 		1878.6
(-1)
P 		2277.3
(0.3)
P 		3185
(3)
P 		1984
C 2124.9
(-1.2)
P 		1752.5
(-2.6)
P 		1645.8
(-3)
P 		1713.3
(-2.7)
P 		2109.3
(-1.2)
P 		3207.7
(3)
P 		1562
avr 2117.2
(-0.7) 		1909.3
(-1.4) 		1456
(-3) 		1780.2
(-1.9) 		2281.3
(-0.1) 		3172.2
(3) 		1716
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 523
1 2 3 4 range
A 726.4
(3)
P 		254.3
(-2.6)
P 		374.3
(-1.2)
P 		223.2
(-3)
P 		503
B 688.4
(3)
P 		376.2
(-2.2)
P 		330.5
(-3)
P 		556.6
(0.8)
P 		358
C 745.9
(3)
P 		487.4
(-1.2)
P 		618.6
(0.9)
P 		375
(-3)
P 		371
avr 720.2
(3) 		372.6
(-3) 		441.1
(-1.8) 		384.9
(-2.8) 		348

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 896
1 2 3 4 5 6 range
A 395
(-2.1)
P 		720.2
(2.4)
P 		331.2
(-3)
P 		443.5
(-1.4)
P 		741.5
(2.7)
P 		760.6
(3)
P 		429
B 725
(-0.1)
P 		649.1
(-0.6)
P 		291.7
(-3)
P 		497.6
(-1.6)
P 		925.2
(1.2)
P 		1187.7
(3)
P 		896
C 787.6
(0.6)
P 		534.7
(-1.4)
P 		418.9
(-2.4)
P 		344.5
(-3)
P 		682.5
(-0.2)
P 		1073.8
(3)
P 		729
avr 635.9
(-0.4) 		634.7
(-0.4) 		347.3
(-3) 		428.5
(-2.3) 		783.1
(1) 		1007.4
(3) 		660
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175808_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19902
Sequence Type Exemplar sequence
Representative Public ID 3694830 NCBI
Target Description g3694830 /REP_DB=GenBank Identifier /GB=AF072380.1 /PROTEIN_GB=AAC62467.1 /CHR=5 /FEA=Mapped Transcript /DEF=Akt-PKB serine-threonine kinase
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028475(11), NM_001028474(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028475 NCBI Caenorhabditis elegans AKT kinase family member (akt-1) (akt-1) mRNA, complete cds. 11/11 None
NM_001028474 NCBI Caenorhabditis elegans AKT kinase family member (akt-1) (akt-1) mRNA, complete cds. 11/11 None
C12D8.10b.2 ENSEMBL cdna:known chromosome:CEL140:V:10251486:10255476:1 gene:C12D8.10 11/11 A 
C12D8.10b.1 ENSEMBL cdna:known chromosome:CEL140:V:10251488:10255476:1 gene:C12D8.10 11/11 A 
C12D8.10a ENSEMBL cdna:known chromosome:CEL140:V:10251488:10255476:1 gene:C12D8.10 11/11 A 
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179424 3 2 NM_001028474 175808_s_at (11), 194058_s_at (11)
NM_001028475 175808_s_at (11), 194058_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:10251487-10255476(+) 100.0 100.0
Public Domain and Genome References
Ensembl C12D8.10
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA98238 Hypothetical protein C12D8.10b [Caenorhabditis elegans] ref|NP_001023646.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62467.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAA98240 Hypothetical protein C12D8.10a [Caenorhabditis elegans] ref|NP_001023645.1| AKT kinase family member (akt-1) [Caenorhabditis elegans] gb|AAC62466.1| Akt/PKB serine/threonine kinase [Caenorhabditis elegans] 0.0
blast CAE64774 Hypothetical protein CBG09565 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-68
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 3.0E-69
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 1.0E-106
hanks 1.3.3 AGC group; AGC III RAC(Akt); DmRAC 1.0E-108
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 4.3E-94
Pfam IPR001849 EMBL-EBI Pleckstrin-like 4.9E-23
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Pfam IPR000719 EMBL-EBI Protein kinase 3.1E-93
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.9E-22
Sequence
Target Sequence 
>C. ELEGANS:175808_S_AT
taattgcgattttagcctgttttgcagactgtagaaaactgacccctttgtctgacattc
agatatccttttttaaacaaatatttagtcgttacagtgactatattttgatctattctc
agtaaatatcgaattaaaatgagaaatctctttgttttcatctcttcctgttctgcctcc
ttccctttgtatactatcctttttattcacctgagaccgtagttctttccatttcaaaat
tccatcatcgttttgtaatattactcacaagctacggttgtgaaaaccaaatagcgccat
cattcgtggatgtactatgttcctatatca

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATTGCGATTTTAGCCTGTTTTGC 680 631 1731 Antisense
GACTGTAGAAAACTGACCCCTTTGT 552 369 1757 Antisense
CTGACCCCTTTGTCTGACATTCAGA 198 239 1769 Antisense
GACATTCAGATATCCTTTTTTAAAC 271 369 1784 Antisense
GAAATCTCTTTGTTTTCATCTCTTC 366 363 1873 Antisense
TCCCTTTGTATACTATCCTTTTTAT 486 597 1912 Antisense
ATCCTTTTTATTCACCTGAGACCGT 507 37 1926 Antisense
TGAGACCGTAGTTCTTTCCATTTCA 670 563 1942 Antisense
AAATTCCATCATCGTTTTGTAATAT 643 115 1967 Antisense
AAACCAAATAGCGCCATCATTCGTG 533 123 2014 Antisense
GTGGATGTACTATGTTCCTATATCA 641 489 2036 Antisense
Sequence Source GenBank