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[175791_at]

Description g6425162 /REP_DB=GenBank Identifier /GB=AF139093.2 /PROTEIN_GB=AAD48771.1 /CHR=5 /FEA=Mapped Transcript /DEF=muscarinic acetylcholine receptor
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 36      4grp_highest_8_8 > 46      4grp_high_8_8 > 02      6grp_highest_nonorm > 40      6grp_total > 26      4grp_total > 14      6grp_highest_sub > Cu      6grp_cgc > 24      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 965
1 2 3 4 range
A 792.6
(1.8)
P 		300.3
(-2.4)
P 		234.2
(-3)
P 		936.9
(3)
P 		703
B 762.1
(0.1)
P 		353.1
(-2.7)
P 		309.1
(-3)
P 		1199.5
(3)
P 		890
C 839
(1)
P 		397
(-3)
P 		536.3
(-1.7)
P 		1061.8
(3)
P 		665
avr 797.9
(0.8) 		350.1
(-3) 		359.9
(-2.9) 		1066.1
(3) 		716

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 730
1 2 3 4 5 6 range
A 968.9
(3)
P 		692.6
(0.4)
P 		602
(-0.4)
P 		379.1
(-2.5)
P 		325.6
(-3)
P 		514
(-1.2)
P 		643
B 1015.8
(3)
P 		1003.3
(2.9)
P 		578.6
(-0.9)
P 		520.3
(-1.4)
P 		342
(-3)
P 		774.7
(0.9)
P 		674
C 877.7
(2)
P 		1001.7
(3)
P 		553.9
(-0.8)
P 		352.1
(-2.4)
P 		285.5
(-3)
P 		496.3
(-1.2)
P 		716
avr 954.1
(3) 		899.2
(2.5) 		578.2
(-0.5) 		417.2
(-2.1) 		317.7
(-3) 		595
(-0.4) 		636
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 227
1 2 3 4 range
A 288.6
(3)
P 		65.2
(-2.9)
P 		61.2
(-3)
P 		137.1
(-1)
P 		227
B 243
(2.6)
P 		87.2
(-2.5)
P 		71.6
(-3)
P 		255.6
(3)
P 		184
C 277.7
(3)
P 		114.1
(-3)
P 		186.9
(-0.3)
P 		179
(-0.6)
P 		164
avr 269.8
(3) 		88.8
(-3) 		106.6
(-2.4) 		190.6
(0.4) 		181

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 283
1 2 3 4 5 6 range
A 178.6
(0.3)
P 		246.3
(3)
P 		131.1
(-1.6)
P 		96.2
(-3)
P 		98.3
(-2.9)
P 		125.1
(-1.8)
P 		150
B 353.5
(3)
P 		334
(2.5)
P 		140.5
(-2.9)
P 		137.8
(-3)
P 		139
(-3)
P 		288.9
(1.2)
P 		216
C 325.3
(3)
P 		305.7
(2.5)
P 		141
(-1.3)
P 		70.8
(-3)
P 		92.4
(-2.5)
P 		166.1
(-0.8)
P 		255
avr 285.8
(2.7) 		295.3
(3) 		137.5
(-1.9) 		101.6
(-3) 		109.9
(-2.7) 		193.4
(-0.2) 		194
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175791_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19922
Sequence Type Exemplar sequence
Representative Public ID 6425162 NCBI
Target Description g6425162 /REP_DB=GenBank Identifier /GB=AF139093.2 /PROTEIN_GB=AAD48771.1 /CHR=5 /FEA=Mapped Transcript /DEF=muscarinic acetylcholine receptor
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029065(11), NM_001029064(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029065 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-3) (gar-3) mRNA, complete cds. 11/11 None
NM_001029064 NCBI Caenorhabditis elegans G-protein-linked Acetylcholine Receptor family member (gar-3) (gar-3) mRNA, complete cds. 11/11 None
Y40H4A.1b.1 ENSEMBL cdna:known chromosome:CEL140:V:14567341:14572585:-1 gene:Y40H4A.1 11/11 None
Y40H4A.1a ENSEMBL cdna:known chromosome:CEL140:V:14567407:14582308:-1 gene:Y40H4A.1 11/11 A 
Y40H4A.1b.2 ENSEMBL cdna:known chromosome:CEL140:V:14567415:14572583:-1 gene:Y40H4A.1 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000009304 4/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:14567406-14582310(-) 98.24 100.0
Public Domain and Genome References
Ensembl Y40H4A.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAE47465 Hypothetical protein Y40H4A.1b [Caenorhabditis elegans] emb|CAE47471.1| Hypothetical protein Y40H4A.1b [Caenorhabditis elegans] ref|NP_001024236.1| G-protein-linked Acetylcholine Receptor family member (gar-3) [Caenorhabditis elegans] gb|AAP97492.1| muscarinic acetylcholine receptor large isoform; GAR-3a [Caenorhabditis elegans] sp|Q9U7D5|ACM3_CAEEL Muscarinic acetylcholine receptor gar-3 (G-protein linked acetylcholine receptor 3) 0.0
blast CAC42272 Hypothetical protein Y40H4A.1a [Caenorhabditis elegans] emb|CAA22301.2| Hypothetical protein Y40H4A.1a [Caenorhabditis elegans] ref|NP_001024235.1| G-protein-linked Acetylcholine Receptor family member (gar-3) [Caenorhabditis elegans] gb|AAD48771.1| muscarinic acetylcholine receptor [Caenorhabditis elegans] 0.0
blast CAE60870 Hypothetical protein CBG04581 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.1E-89
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 6.4E-90
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024236.1 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
NP_001024235.1 7 68-90,102-124,139-161,182-204,228-250,500-522,532-554
Y40H4A.1b.1 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
Y40H4A.1a 7 68-90,102-124,139-161,182-204,228-250,500-522,532-554
Y40H4A.1b.2 7 68-90,102-124,139-161,182-204,228-250,526-548,558-580
Sequence
Target Sequence 
>C. ELEGANS:175791_AT
tccgacgcaactagagactcaatcaatcagtacaaaaatgtgcacaactccctttgccaa
gatctcgaaacacttgaaacagtatattagatgcatttgaaaaccggtccaacatcatca
ttttctaatttttttcaacttttttcttaacttttttcaaattagttcgaaatatttggt
cttgtttatcaacattcttctcaccgaatcattgctcattcattgttgacgggt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCCGACGCAACTAGAGACTCAATCA 224 595 1904 Antisense
AAAAATGTGCACAACTCCCTTTGCC 656 129 1937 Antisense
TCCCTTTGCCAAGATCTCGAAACAC 425 597 1952 Antisense
AGATCTCGAAACACTTGAAACAGTA 88 65 1963 Antisense
GATGCATTTGAAAACCGGTCCAACA 86 397 1993 Antisense
AAAACCGGTCCAACATCATCATTTT 182 131 2003 Antisense
CAACATCATCATTTTCTAATTTTTT 522 189 2013 Antisense
AATATTTGGTCTTGTTTATCAACAT 510 173 2074 Antisense
AACATTCTTCTCACCGAATCATTGC 243 139 2094 Antisense
TCTTCTCACCGAATCATTGCTCATT 525 617 2099 Antisense
CATTGCTCATTCATTGTTGACGGGT 551 217 2113 Antisense
Sequence Source GenBank