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[175781_s_at]

Description g1778589 /REP_DB=GenBank Identifier /GB=U82935.1 /PROTEIN_GB=AAB40868.1 /CHR=X /FEA=Mapped Transcript /DEF=protein kinase C2 A isoform
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 51      4grp_high_8_8 > 25      6grp_highest_nonorm > 70      6grp_total > 07      4grp_total > 47      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 522
1 2 3 4 range
A 684.7
(3)
P 		224.5
(-2.3)
P 		162.5
(-3)
P 		228.2
(-2.2)
P 		522
B 641.9
(3)
P 		250.3
(-2.2)
P 		194.2
(-3)
P 		296.7
(-1.6)
P 		448
C 630.1
(3)
P 		273.6
(-2.9)
P 		265
(-3)
P 		333.9
(-1.9)
P 		365
avr 652.2
(3) 		249.5
(-2.4) 		207.2
(-3) 		286.3
(-1.9) 		445

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 372
1 2 3 4 5 6 range
A 532.3
(3)
P 		308.8
(-1.1)
P 		204.3
(-3)
P 		256.6
(-2)
P 		219.4
(-2.7)
P 		225
(-2.6)
P 		328
B 538.5
(3)
P 		312.7
(-0.6)
P 		166.9
(-3)
P 		214.8
(-2.2)
P 		211
(-2.3)
P 		212
(-2.3)
P 		372
C 517.1
(3)
P 		405.6
(1)
P 		272.6
(-1.4)
P 		209.7
(-2.6)
P 		258.3
(-1.7)
P 		187
(-3)
P 		330
avr 529.3
(3) 		342.4
(-0.5) 		214.6
(-2.9) 		227
(-2.6) 		229.6
(-2.6) 		208
(-3) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 216
1 2 3 4 range
A 249.3
(3)
P 		48.7
(-2.6)
P 		42.5
(-2.7)
P 		33.4
(-3)
P 		216
B 204.7
(3)
P 		61.8
(-2.4)
P 		45
(-3)
P 		63.2
(-2.3)
P 		160
C 208.6
(3)
P 		78.7
(-2.1)
P 		92.3
(-1.6)
P 		56.3
(-3)
P 		152
avr 220.9
(3) 		63.1
(-2.6) 		59.9
(-2.7) 		51
(-3) 		170

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 151
1 2 3 4 5 6 range
A 98.1
(1.9)
P 		109.8
(3)
P 		44.5
(-3)
P 		65.1
(-1.1)
P 		66.2
(-1)
P 		54.8
(-2.1)
P 		65
B 187.4
(3)
P 		104.1
(-0.4)
P 		40.5
(-3)
P 		56.9
(-2.3)
P 		85.7
(-1.2)
P 		79.1
(-1.4)
P 		147
C 191.7
(3)
P 		123.8
(0.3)
P 		69.4
(-1.9)
P 		42.2
(-3)
P 		83.6
(-1.3)
P 		62.6
(-2.2)
P 		150
avr 159.1
(3) 		112.6
(0.4) 		51.5
(-3) 		54.7
(-2.8) 		78.5
(-1.5) 		65.5
(-2.2) 		108
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175781_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19983
Sequence Type Exemplar sequence
Representative Public ID 1778589 NCBI
Target Description g1778589 /REP_DB=GenBank Identifier /GB=U82935.1 /PROTEIN_GB=AAB40868.1 /CHR=X /FEA=Mapped Transcript /DEF=protein kinase C2 A isoform
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029345(11), NM_001029347(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029345 NCBI Caenorhabditis elegans Protein Kinase C family member (pkc-2) (pkc-2) mRNA, complete cds. 11/11 None
NM_001029347 NCBI Caenorhabditis elegans Protein Kinase C family member (pkc-2) (pkc-2) mRNA, complete cds. 11/11 None
SNAP00000040112 ENSEMBL cdna:SNAP chromosome:CEL140:X:9385185:9385656:1 11/11 None
E01H11.1a.2 ENSEMBL cdna:known chromosome:CEL140:X:9371060:9385965:1 gene:E01H11.1 11/11 A 
E01H11.1a.1 ENSEMBL cdna:known chromosome:CEL140:X:9371086:9386328:1 gene:E01H11.1 11/11 A 
E01H11.1c ENSEMBL cdna:known chromosome:CEL140:X:9376201:9385656:1 gene:E01H11.1 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
181166 3 2 NM_001029345 175780_at (11), 175781_s_at (11)
NM_001029346 175779_at (11), 192006_s_at (11)
NM_001029347 175781_s_at (11), 186204_at (11), 192005_at (11), 192006_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:9371059-9385965(+) 100.0 100.0
Public Domain and Genome References
Ensembl E01H11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAM51503 Protein kinase c protein 2, isoform a [Caenorhabditis elegans] ref|NP_001024516.1| Protein Kinase C family member (pkc-2) [Caenorhabditis elegans] 0.0
blast AAB40868 protein kinase C2 A isoform [Caenorhabditis elegans] 0.0
blast AAM51504 Protein kinase c protein 2, isoform b [Caenorhabditis elegans] ref|NP_001024517.1| Protein Kinase C family member (pkc-2) [Caenorhabditis elegans] sp|P90980|KPC2_CAEEL Protein kinase C-like 2 (PKC2) 0.0
blast AAA68709 Protein kinase c protein 2, isoform c [Caenorhabditis elegans] ref|NP_001024518.1| Protein Kinase C family member (pkc-2) [Caenorhabditis elegans] 0.0
blast T15903 protein kinase C homolog - Caenorhabditis elegans 0.0
blast CAE69829 Hypothetical protein CBG16150 [Caenorhabditis briggsae] 0.0
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 2.0E-68
hanks 1.2.3 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; DPKC53b 1.0E-125
hanks 1.2.3 AGC group; AGC I Diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family; DPKC53b 1.0E-126
Protein Domains
Database ID Description E-Value
Pfam IPR000008 EMBL-EBI C2 domain 1.6E-37
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.0E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.2E-24
Pfam IPR000719 EMBL-EBI Protein kinase 3.0E-81
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 2.2E-35
Pfam IPR000008 EMBL-EBI C2 domain 1.6E-37
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 2.0E-24
Pfam IPR002219 EMBL-EBI Protein kinase C, phorbol ester/diacylglycerol binding 6.2E-24
Pfam IPR000719 EMBL-EBI Protein kinase 3.0E-81
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 2.7E-21
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 7.5E-21
Pfam IPR000961 EMBL-EBI Protein kinase C-terminal domain 1.8E-21
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_001024518.1 3 588-610,824-843,848-870
E01H11.1c 3 588-610,824-843,848-870
Sequence
Target Sequence 
>C. ELEGANS:175781_S_AT
cgccgatgatacttccaacttcgattcagagtttactcacgaggtccctaagcttactcc
gattgaccgtctattcctcatgaatctggatcaaactgaattcgaaggattctcttttgt
caacccagaatatgttcaag

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CGCCGATGATACTTCCAACTTCGAT 455 257 1934 Antisense
GATACTTCCAACTTCGATTCAGAGT 686 423 1941 Antisense
TCAGAGTTTACTCACGAGGTCCCTA 405 621 1959 Antisense
TACTCACGAGGTCCCTAAGCTTACT 271 643 1967 Antisense
TAAGCTTACTCCGATTGACCGTCTA 101 635 1982 Antisense
TTACTCCGATTGACCGTCTATTCCT 414 681 1987 Antisense
GACCGTCTATTCCTCATGAATCTGG 196 383 1998 Antisense
TCCTCATGAATCTGGATCAAACTGA 687 581 2008 Antisense
TTCGAAGGATTCTCTTTTGTCAACC 369 693 2034 Antisense
AGGATTCTCTTTTGTCAACCCAGAA 360 57 2039 Antisense
TTTGTCAACCCAGAATATGTTCAAG 153 667 2049 Antisense
Sequence Source GenBank