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[175772_s_at]

Description g2317844 /REP_DB=GenBank Identifier /GB=U15167.1 /PROTEIN_GB=AAB66360.1 /CHR=3 /FEA=Mapped Transcript /DEF=serotonin receptor precursor
Cluster 6grp_highest_10_10 > 70      6grp_high1_10_10 > 17      4grp_highest_8_8 > 61      4grp_high_8_8 > 17      6grp_highest_nonorm > 50      6grp_total > 06      4grp_total > 30      6grp_highest_sub > De      6grp_cgc > 12      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 922
1 2 3 4 range
A 1190.3
(3)
P 		563.8
(-1.4)
P 		340.5
(-3)
P 		623.1
(-1)
P 		850
B 1262.5
(3)
P 		449.9
(-2.5)
P 		379.2
(-3)
P 		942.8
(0.8)
P 		883
C 1189
(3)
P 		596.9
(-1.2)
P 		342.2
(-3)
P 		871.5
(0.8)
P 		847
avr 1213.9
(3) 		536.9
(-1.7) 		354
(-3) 		812.5
(0.2) 		860

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 582
1 2 3 4 5 6 range
A 838.8
(3)
P 		555.8
(0)
P 		367
(-1.9)
P 		266.7
(-3)
P 		377.8
(-1.8)
P 		293.9
(-2.7)
P 		572
B 825
(3)
P 		627.6
(0.7)
P 		301.5
(-3)
P 		375.6
(-2.2)
P 		405.8
(-1.8)
P 		332.4
(-2.6)
P 		524
C 848.8
(3)
P 		504.2
(-1)
P 		343.9
(-2.8)
P 		329.8
(-3)
P 		357.4
(-2.7)
P 		389.3
(-2.3)
P 		519
avr 837.5
(3) 		562.5
(-0.2) 		337.5
(-2.8) 		324
(-3) 		380.3
(-2.3) 		338.5
(-2.8) 		514
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 346
1 2 3 4 range
A 433.4
(3)
P 		122.4
(-2.4)
P 		89
(-3)
P 		91.2
(-3)
P 		344
B 402.6
(3)
P 		111.1
(-2.6)
P 		87.9
(-3)
P 		200.9
(-0.8)
P 		315
C 393.6
(3)
P 		171.6
(-1.9)
P 		119.3
(-3)
P 		146.9
(-2.4)
P 		274
avr 409.9
(3) 		135
(-2.3) 		98.7
(-3) 		146.3
(-2.1) 		311

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 248
1 2 3 4 5 6 range
A 154.6
(1)
P 		197.7
(3)
P 		79.9
(-2.4)
P 		67.6
(-3)
P 		114
(-0.9)
P 		71.6
(-2.8)
P 		130
B 287.1
(3)
P 		208.9
(0.8)
P 		73.2
(-3)
P 		99.5
(-2.3)
P 		164.9
(-0.4)
P 		123.9
(-1.6)
P 		214
C 314.6
(3)
P 		153.9
(-0.9)
P 		87.5
(-2.5)
P 		66.3
(-3)
P 		115.6
(-1.8)
P 		130.3
(-1.5)
P 		248
avr 252.1
(3) 		186.8
(0.8) 		80.2
(-2.9) 		77.8
(-3) 		131.5
(-1.2) 		108.6
(-1.9) 		174
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175772_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19834
Sequence Type Exemplar sequence
Representative Public ID 2317844 NCBI
Target Description g2317844 /REP_DB=GenBank Identifier /GB=U15167.1 /PROTEIN_GB=AAB66360.1 /CHR=3 /FEA=Mapped Transcript /DEF=serotonin receptor precursor
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_065051(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_065051 NCBI Caenorhabditis elegans SERotonin/octopamine receptor family member (ser-4) (ser-4) mRNA, complete cds. 11/11 None
Y22D7AR.13.1 ENSEMBL cdna:known chromosome:CEL140:III:1723183:1733090:-1 gene:Y22D7AR.13 11/11 None
Y22D7AR.13.2 ENSEMBL cdna:known chromosome:CEL140:III:1723189:1733092:-1 gene:Y22D7AR.13 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
GENEFINDER00000025846 1/11 Cross Hyb Matching Probes None
SNAP00000025839 6/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIII:1723188-1733092(-) 100.0 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ser-4
Ensembl Y22D7AR.13
Entrez Gene 175322 Entrez gene
SwissProt O18512 EMBL-EBI
WormBase CE29370 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7186 G-protein coupled receptor protein signaling pathway inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
16021 integral to membrane inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
1584 rhodopsin-like receptor activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAK29875 Serotonin/octopamine receptor family protein 4 [Caenorhabditis elegans] ref|NP_497452.1| SERotonin/octopamine receptor family member (ser-4) [Caenorhabditis elegans] gb|AAB66360.1| serotonin receptor precursor [Caenorhabditis elegans] 0.0
blast CAE69091 Hypothetical protein CBG15112 [Caenorhabditis briggsae] 0.0
blast AAO45883 serotonin receptor protein [Haemonchus contortus] 1.0E-141
Protein Domains
Database ID Description E-Value
Pfam IPR000276 EMBL-EBI Rhodopsin-like GPCR superfamily 1.3E-90
Trans Membrane
ID Number Of Domains Domain Boundaries
NP_497452.1 7 55-77,89-111,126-148,168-190,215-237,369-391,406-425
Y22D7AR.13.1 7 55-77,89-111,126-148,168-190,215-237,369-391,406-425
Y22D7AR.13.2 7 55-77,89-111,126-148,168-190,215-237,369-391,406-425
Sequence
Target Sequence 
>C. ELEGANS:175772_S_AT
attattacaggtacattcgttgcgtgttggaccccatttttcctggtctctatataccgc
ccaatttgcggatgtcaaatttctccggtgctggagcaagttacactgtggctgggttac
ctaaactcggccctaaatccgataatttatacggtattttcacaagattttcgcgccgca
ttcaaacgaatcatcaaacggatgtgccttattcacgattattgattttttctcaatccc
ataaaatttcgatttttcgaagaaaaacaaatcgataatcaacacacctgtattgtctcc
cttatcattccacatttgtcccatacgggttttgtacaacacacattttctcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATTATTACAGGTACATTCGTTGCGT 490 13 1141 Antisense
GGTATTTTCACAAGATTTTCGCGCC 531 505 1293 Antisense
AGATTTTCGCGCCGCATTCAAACGA 410 65 1305 Antisense
CGCGCCGCATTCAAACGAATCATCA 359 255 1312 Antisense
ATCAAACGGATGTGCCTTATTCACG 522 27 1333 Antisense
GGATGTGCCTTATTCACGATTATTG 235 515 1340 Antisense
TTGATTTTTTCTCAATCCCATAAAA 529 703 1362 Antisense
AATCAACACACCTGTATTGTCTCCC 622 169 1417 Antisense
TTCCACATTTGTCCCATACGGGTTT 564 699 1448 Antisense
TCCCATACGGGTTTTGTACAACACA 556 597 1459 Antisense
GTTTTGTACAACACACATTTTCTCC 683 445 1469 Antisense
Sequence Source GenBank