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[175760_at]

Description g3372520 /REP_DB=GenBank Identifier /GB=AF051401.1 /PROTEIN_GB=AAC28321.1 /CHR=4 /FEA=Mapped Transcript /DEF=fibulin-1 isoform D precursor
Cluster 6grp_highest_10_10 > 62      6grp_high1_10_10 > 26      4grp_highest_8_8 > 47      4grp_high_8_8 > 11      6grp_highest_nonorm > 80      6grp_total > 27      4grp_total > 13      6grp_highest_sub > Cu      6grp_cgc > 05      6grp_high1_sub > De      6grp_cgc_sub > Cu      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 409
1 2 3 4 range
A 294.6
(0.9)
P 		139.1
(-3)
P 		137.6
(-3)
P 		377.7
(3)
P 		240
B 225.3
(-1.1)
P 		153.9
(-2.1)
P 		94
(-3)
P 		502.7
(3)
P 		409
C 297.7
(0.5)
P 		194.2
(-1.3)
P 		95.9
(-3)
P 		440
(3)
P 		344
avr 272.5
(-0) 		162.4
(-2) 		109.2
(-3) 		440.1
(3) 		331

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 170
1 2 3 4 5 6 range
A 229.3
(3)
P 		223.1
(2.5)
P 		171.9
(-1.3)
P 		159
(-2.3)
P 		152.3
(-2.8)
P 		149.3
(-3)
P 		80
B 220.4
(2.7)
P 		223.9
(3)
P 		179.7
(-1.3)
P 		172
(-2.1)
P 		162.5
(-3)
P 		213.7
(2)
P 		61
C 311.2
(3)
P 		233.2
(0.2)
P 		178.9
(-1.7)
P 		141.3
(-3)
P 		204.4
(-0.8)
P 		240.5
(0.5)
P 		170
avr 253.6
(3) 		226.7
(1.3) 		176.8
(-1.8) 		157.4
(-3) 		173.1
(-2) 		201.2
(-0.3) 		96
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 86
1 2 3 4 range
A 107.3
(3)
P 		30.2
(-3)
P 		36
(-2.5)
P 		55.3
(-1)
P 		77
B 71.8
(0.5)
P 		38
(-1.9)
P 		21.8
(-3)
P 		107.1
(3)
P 		85
C 98.6
(3)
P 		55.8
(-0.9)
P 		33.4
(-3)
P 		74.2
(0.8)
P 		65
avr 92.6
(3) 		41.3
(-1.9) 		30.4
(-3) 		78.9
(1.7) 		62

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 87
1 2 3 4 5 6 range
A 42.3
(-2.2)
P 		79.4
(3)
P 		37.4
(-2.9)
P 		40.3
(-2.5)
P 		46
(-1.7)
P 		36.4
(-3)
P 		43
B 76.7
(2.5)
P 		74.5
(2.1)
P 		43.6
(-3)
P 		45.6
(-2.7)
P 		66
(0.7)
P 		79.7
(3)
P 		36
C 115.3
(3)
P 		71.1
(-0.1)
P 		45.5
(-1.8)
P 		28.4
(-3)
P 		66.1
(-0.4)
P 		80.5
(0.6)
P 		87
avr 78.1
(3) 		75
(2.5) 		42.2
(-2.4) 		38.1
(-3) 		59.4
(0.2) 		65.5
(1.1) 		40
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175760_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19844
Sequence Type Exemplar sequence
Representative Public ID 3372520 NCBI
Target Description g3372520 /REP_DB=GenBank Identifier /GB=AF051401.1 /PROTEIN_GB=AAC28321.1 /CHR=4 /FEA=Mapped Transcript /DEF=fibulin-1 isoform D precursor
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069293(11), NM_001028066(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069293 NCBI Caenorhabditis elegans FiBuLin family member (fbl-1) (fbl-1) mRNA, complete cds. 11/11 None
NM_001028066 NCBI Caenorhabditis elegans FiBuLin family member (fbl-1) (fbl-1) mRNA, complete cds. 11/11 None
SNAP00000033935 ENSEMBL cdna:SNAP chromosome:CEL140:IV:9545262:9546072:1 11/11 None
GENEFINDER00000033951 ENSEMBL cdna:GeneFinder chromosome:CEL140:IV:9544153:9546072:1 11/11 None
F56H11.1b ENSEMBL cdna:known chromosome:CEL140:IV:9540785:9546072:1 gene:F56H11.1 11/11 None
F56H11.1c ENSEMBL cdna:known chromosome:CEL140:IV:9540829:9546060:1 gene:F56H11.1 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
177788 3 2 NM_001028065 188908_s_at (11)
NM_001028066 175760_at (11)
NM_069293 175760_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:9540834-9546078(+) 100.0 100.0
Public Domain and Genome References
Ensembl F56H11.1
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast CAA92483 Hypothetical protein F56H11.1c [Caenorhabditis elegans] emb|CAA92962.2| Hypothetical protein F56H11.1c [Caenorhabditis elegans] gb|AAW34127.1| fibulin-1D splice variant [Caenorhabditis elegans] ref|NP_501694.2| FiBuLin family member (fbl-1) [Caenorhabditis elegans] sp|O77469|FBLN1_CAEEL Fibulin-1 precursor 0.0
blast CAC35827 Hypothetical protein F56H11.1b [Caenorhabditis elegans] emb|CAC35818.1| Hypothetical protein F56H11.1b [Caenorhabditis elegans] ref|NP_001023237.1| FiBuLin family member (fbl-1) [Caenorhabditis elegans] gb|AAC28324.1| fibulin-1 isoform D precursor [Caenorhabditis elegans] gb|AAC28321.1| fibulin-1 isoform D precursor [Caenorhabditis elegans] 0.0
blast CAE72775 Hypothetical protein CBG20030 [Caenorhabditis briggsae] 0.0
blast AAC24035 fibulin-1D precursor [Caenorhabditis elegans] pir||T43210 fibulin-1D precursor - Caenorhabditis elegans (fragment) 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR006209 EMBL-EBI EGF-like domain 3.5E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 7.9E-9
Pfam IPR006209 EMBL-EBI EGF-like domain 4.3E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 5.9E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 3.5E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 7.9E-9
Pfam IPR006209 EMBL-EBI EGF-like domain 4.3E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 5.9E-6
Pfam IPR006209 EMBL-EBI EGF-like domain 3.5E-8
Pfam IPR006209 EMBL-EBI EGF-like domain 7.9E-9
Pfam IPR006209 EMBL-EBI EGF-like domain 4.3E-7
Pfam IPR006209 EMBL-EBI EGF-like domain 5.9E-6
Sequence
Target Sequence 
>C. ELEGANS:175760_AT
ccaaattgctgatggttattcttgcatcaaagtgtgctcaacagaagataccgaatgtct
tggaaatcatacacgagaagttctttatcaattcagagcagtcccatcattgaaaacaat
tatctcaccgattgaagtgtcaagaattgttacacatatgggagttccattttcagttga
ttacaacttggattatgttggacaaaggcactttagaattgttcaagaaagaaatattgg
tatcgttcaattggtaaaaccaattagtgggcctacagtcgaaacaataaaagtgaatat
tcacacaaaatcccgaactgga

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CCAAATTGCTGATGGTTATTCTTGC 585 275 1701 Antisense
ATTCTTGCATCAAAGTGTGCTCAAC 599 11 1718 Antisense
AGATACCGAATGTCTTGGAAATCAT 520 63 1746 Antisense
GAAATCATACACGAGAAGTTCTTTA 403 363 1763 Antisense
TTATCAATTCAGAGCAGTCCCATCA 391 679 1785 Antisense
CAGAGCAGTCCCATCATTGAAAACA 12 207 1794 Antisense
TTATCTCACCGATTGAAGTGTCAAG 303 679 1820 Antisense
ATATGGGAGTTCCATTTTCAGTTGA 457 19 1856 Antisense
GGATTATGTTGGACAAAGGCACTTT 614 507 1890 Antisense
TTAGTGGGCCTACAGTCGAAACAAT 105 591 1964 Antisense
TATTCACACAAAATCCCGAACTGGA 603 663 1998 Antisense
Sequence Source GenBank