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[175751_s_at] myosin heavy chain

Description g1477558 /REP_DB=GenBank Identifier /GB=U49263.1 /PROTEIN_GB=AAC47238.1 /CHR=1 /FEA=Mapped Transcript /DEF=non-muscle myosin heavy chain II
Cluster 6grp_highest_10_10 > 84      6grp_high1_10_10 > 57      4grp_highest_8_8 > 58      4grp_high_8_8 > 33      6grp_highest_nonorm > 58      6grp_total > 59      4grp_total > 47      6grp_highest_sub > Cu      6grp_cgc > 28      6grp_high1_sub > Cu      6grp_cgc_sub > Cu      6grp_total_sub > Cup      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 890
1 2 3 4 range
A 2413.4
(3)
P 		1749.2
(-2.8)
P 		1731.6
(-3)
P 		1840.5
(-2)
P 		682
B 2245.6
(3)
P 		1767.7
(-1)
P 		1523.5
(-3)
P 		1646.5
(-2)
P 		722
C 2074.1
(3)
P 		1655.6
(-1.7)
P 		1658.6
(-1.7)
P 		1542.1
(-3)
P 		532
avr 2244.4
(3) 		1724.2
(-2.1) 		1637.9
(-3) 		1676.4
(-2.6) 		607

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 2076
1 2 3 4 5 6 range
A 2922.8
(1.1)
P 		2102.3
(-1.3)
P 		1505.2
(-3)
P 		1999
(-1.6)
P 		2631.2
(0.3)
P 		3581.1
(3)
P 		2076
B 2834.6
(0.9)
P 		2106.8
(-1.8)
P 		1786.2
(-3)
P 		2322.2
(-1)
P 		2071.5
(-1.9)
P 		3396.4
(3)
P 		1610
C 2672.4
(2.3)
P 		2012.3
(-3)
P 		2461.4
(0.6)
P 		2053.8
(-2.7)
P 		2137.9
(-2)
P 		2765.3
(3)
P 		753
avr 2809.9
(1) 		2073.8
(-2.3) 		1917.6
(-3) 		2125
(-2.1) 		2280.2
(-1.4) 		3247.6
(3) 		1330
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 619
1 2 3 4 range
A 878.7
(3)
P 		379.7
(-1.9)
P 		452.7
(-1.2)
P 		269.4
(-3)
P 		609
B 716.1
(3)
P 		436.6
(-1.6)
P 		353
(-3)
P 		350.9
(-3)
P 		365
C 686.6
(3)
P 		476
(0)
P 		578.1
(1.5)
P 		260
(-3)
P 		427
avr 760.5
(3) 		430.8
(-1.2) 		461.3
(-0.8) 		293.4
(-3) 		467

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 939
1 2 3 4 5 6 range
A 538.8
(-0.7)
P 		747.7
(1.6)
P 		327.8
(-3)
P 		507
(-1)
P 		794
(2.1)
P 		871.9
(3)
P 		544
B 986.3
(1)
P 		701.3
(-1.1)
P 		433.7
(-3)
P 		615.1
(-1.7)
P 		841.6
(-0.1)
P 		1266.5
(3)
P 		833
C 990.5
(3)
P 		614
(-0.9)
P 		626.5
(-0.8)
P 		413
(-3)
P 		691.8
(-0.1)
P 		925.7
(2.3)
P 		578
avr 838.5
(1) 		687.7
(-0.6) 		462.7
(-3) 		511.7
(-2.5) 		775.8
(0.4) 		1021.4
(3) 		559
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175751_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19677
Sequence Type Exemplar sequence
Representative Public ID 1477558 NCBI
Target Description g1477558 /REP_DB=GenBank Identifier /GB=U49263.1 /PROTEIN_GB=AAC47238.1 /CHR=1 /FEA=Mapped Transcript /DEF=non-muscle myosin heavy chain II
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_059785(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_059785 NCBI Caenorhabditis elegans Non-muscle MYosin family member (nmy-2) (nmy-2) mRNA, complete cds. 11/11 A 
F20G4.3 ENSEMBL cdna:known chromosome:CEL140:I:7934771:7942859:-1 gene:F20G4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrI:7934721-7942790(-) 100.0 100.0
Public Domain and Genome References
Gene Title myosin heavy chain
Gene Symbol nmy-2
Ensembl F20G4.3
Entrez Gene 172562 Entrez gene
SwissProt Q19658 EMBL-EBI
WormBase CE27133 Wormbase
Functional Annotations
Ortholog
ID Title Organism
HG-U133_PLUS_2:217545_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:217545_AT myosin, heavy polypeptide 14 hs
HG-U133A:217545_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:234290_X_AT myosin, heavy polypeptide 14 hs
HG-U133B:234290_X_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:219946_X_AT myosin, heavy polypeptide 14 hs
HG-U133A:219946_X_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:219946_X_AT myosin, heavy polypeptide 14 hs
HU35KSUBA:RC_AA402720_AT myosin, heavy polypeptide 14 hs
U133_X3P:G13376043_3P_A_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.287317.0.S1_3P_X_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.287317.0.S1_3P_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.250389.0.S1_3P_X_AT myosin, heavy polypeptide 14 hs
U133_X3P:HS.250389.0.S1_3P_AT myosin, heavy polypeptide 14 hs
HG-U133A_2:217660_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:217660_AT myosin, heavy polypeptide 14 hs
HG-U133A:217660_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:226988_S_AT myosin, heavy polypeptide 14 hs
HG-U133B:226988_S_AT myosin, heavy polypeptide 14 hs
HG-U133_PLUS_2:232977_X_AT myosin, heavy polypeptide 14 hs
HG-U133B:232977_X_AT myosin, heavy polypeptide 14 hs
HG-U95C:59370_AT myosin, heavy polypeptide 14 hs
MG-U74CV2:168959_R_AT myosin, heavy polypeptide 14 mm
MOUSE430A_2:1428835_AT myosin, heavy polypeptide 14 mm
MOE430A:1428835_AT myosin, heavy polypeptide 14 mm
MOUSE430_2:1428835_AT myosin, heavy polypeptide 14 mm
MU11KSUBA:AA688923_AT myosin, heavy polypeptide 14 mm
MG-U74AV2:93481_AT myosin, heavy polypeptide 14 mm
RAE230B:1384264_AT myosin, heavy polypeptide 14 (predicted) rn
RAT230_2:1384264_AT myosin, heavy polypeptide 14 (predicted) rn
Gene Ontology
GO Cellular Component (view graph)
ID Description Evidence Links
16459 myosin inferred from electronic annotation QuickGO AmiGO
16460 myosin II inferred from sequence similarity QuickGO AmiGO
5819 spindle inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
3774 motor activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAA99931 Hypothetical protein F20G4.3 [Caenorhabditis elegans] emb|CAA99841.2| Hypothetical protein F20G4.3 [Caenorhabditis elegans] ref|NP_492186.3| Non-muscle MYosin family member (nmy-2) [Caenorhabditis elegans] 0.0
blast AAC47238 non-muscle myosin heavy chain II 0.0
blast CAE63725 Hypothetical protein CBG08250 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
Pfam IPR004009 EMBL-EBI Myosin N-terminal SH3-like domain 5.9E-14
Pfam IPR002928 EMBL-EBI Myosin tail 1.0E-126
Pfam IPR001609 EMBL-EBI Myosin head (motor domain) 1.0E-126
Sequence
Target Sequence 
>C. ELEGANS:175751_S_AT
tcagttcgcaactaacctctaaagtttttaattccggattatttttttgtttaatttact
gacctgattgtatccaaacgttcccaattcttatccattcccctatgtttttagaccatg
attgtccccttgatcaacatttccctgcacaatctgctttaatagtattcgccgtgttat
aaatttgcacaatatcgcatgtcacgtcgttcttctcgtttcatgtctatcaccatcatc
acctcagcttcaatatgtatttttatcatttaccgcttcc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TCAGTTCGCAACTAACCTCTAAAGT 392 601 6144 Antisense
TTTACTGACCTGATTGTATCCAAAC 193 665 6198 Antisense
TATCCAAACGTTCCCAATTCTTATC 132 651 6214 Antisense
CTTATCCATTCCCCTATGTTTTTAG 460 219 6233 Antisense
TTAGACCATGATTGTCCCCTTGATC 573 681 6254 Antisense
CTTGATCAACATTTCCCTGCACAAT 669 217 6272 Antisense
ATTTCCCTGCACAATCTGCTTTAAT 144 17 6282 Antisense
GTATTCGCCGTGTTATAAATTTGCA 209 453 6308 Antisense
AATATCGCATGTCACGTCGTTCTTC 270 173 6334 Antisense
ATCACCTCAGCTTCAATATGTATTT 357 29 6381 Antisense
TGTATTTTTATCATTTACCGCTTCC 280 561 6399 Antisense
Sequence Source GenBank