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[175745_at]

Description g3220258 /REP_DB=GenBank Identifier /GB=AF070472.1 /PROTEIN_GB=AAC33275.1 /CHR=4 /FEA=Mapped Transcript /DEF=insulin-like peptide
Cluster 6grp_high1_10_10 > 27      4grp_highest_8_8 > 55      4grp_high_8_8 > 19      6grp_total > 17      4grp_total > 21      6grp_cgc > 11      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 571
1 2 3 4 range
A 582.7
(3)
P 		386.4
(0)
P 		184.1
(-3)
P 		554
(2.6)
P 		399
B 650.4
(1.9)
P 		365.5
(-1.2)
P 		201.1
(-3)
P 		754.7
(3)
P 		554
C 601.6
(2.2)
P 		329.6
(-1.3)
P 		196.6
(-3)
P 		664.7
(3)
P 		468
avr 611.6
(2.4) 		360.5
(-0.8) 		193.9
(-3) 		657.8
(3) 		464

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 373
1 2 3 4 5 6 range
A 543.4
(3)
P 		288.9
(-1.1)
P 		331.4
(-0.4)
P 		233.1
(-2)
P 		170.7
(-3)
P 		244.2
(-1.8)
P 		373
B 491.4
(3)
P 		370.3
(0.3)
P 		218.6
(-3)
M 		284.2
(-1.6)
P 		289.3
(-1.4)
P 		310.6
(-1)
P 		273
C 490.3
(3)
P 		353.6
(0.1)
P 		276.8
(-1.6)
P 		305
(-1)
P 		211.3
(-3)
P 		322
(-0.6)
P 		279
avr 508.4
(3) 		337.6
(-0.6) 		275.6
(-1.9) 		274.1
(-1.9) 		223.8
(-3) 		292.3
(-1.6) 		285
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 166
1 2 3 4 range
A 212.1
(3)
P 		83.9
(-1.7)
P 		48.1
(-3)
P 		81.1
(-1.8)
P 		164
B 207.4
(3)
P 		90.3
(-1.4)
P 		46.6
(-3)
P 		160.8
(1.3)
P 		161
C 199.1
(3)
P 		94.8
(-1.8)
P 		68.5
(-3)
P 		112.1
(-1)
P 		131
avr 206.2
(3) 		89.7
(-1.6) 		54.4
(-3) 		118
(-0.5) 		152

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 130
1 2 3 4 5 6 range
A 100.2
(2.7)
P 		102.7
(3)
P 		72.2
(-0.6)
P 		59.1
(-2.1)
P 		51.5
(-3)
P 		59.5
(-2.1)
P 		51
B 171
(3)
P 		123.3
(0.6)
P 		53.1
(-3)
M 		75.3
(-1.9)
P 		117.5
(0.3)
P 		115.8
(0.2)
P 		118
C 181.7
(3)
P 		107.9
(-0.7)
P 		70.4
(-2.5)
P 		61.3
(-3)
P 		68.4
(-2.6)
P 		107.8
(-0.7)
P 		120
avr 151
(3) 		111.3
(0.2) 		65.2
(-3) 		65.2
(-3) 		79.1
(-2) 		94.4
(-1) 		86
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175745_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19878
Sequence Type Exemplar sequence
Representative Public ID 3220258 NCBI
Target Description g3220258 /REP_DB=GenBank Identifier /GB=AF070472.1 /PROTEIN_GB=AAC33275.1 /CHR=4 /FEA=Mapped Transcript /DEF=insulin-like peptide
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_069525(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_069525 NCBI Caenorhabditis elegans INSulin related family member (ins-1) (ins-1) mRNA, complete cds. 11/11 None
F13B12.5 ENSEMBL cdna:known chromosome:CEL140:IV:10436680:10438382:1 gene:F13B12.5 11/11 None
Annotation Notes
Cross Hybridizing mRNA Matched Probes Nature of Assignment Related Probesets by Grade
SNAP00000009876 2/11 Cross Hyb Matching Probes None
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrIV:10436685-10438388(+) 95.84 100.0
Public Domain and Genome References
Gene Title
Gene Symbol ins-1
Ensembl F13B12.5
Entrez Gene 177936 Entrez gene
SwissProt O76469 EMBL-EBI
WormBase CE27955 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
7582 physiological process inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5576 extracellular region inferred from electronic annotation QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
5179 hormone activity inferred from electronic annotation QuickGO AmiGO
18445 prothoracicotrophic hormone activity inferred from electronic annotation QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast CAC42282 Hypothetical protein F13B12.5 [Caenorhabditis elegans] ref|NP_501926.1| INSulin related family member (ins-1) [Caenorhabditis elegans] gb|AAC33275.1| insulin-like peptide [Caenorhabditis elegans] 7.0E-61
blast CAE63820 Hypothetical protein CBG08370 [Caenorhabditis briggsae] 5.0E-44
Protein Domains
Database ID Description E-Value
Pfam IPR004825 EMBL-EBI Insulin/IGF/relaxin 1.8E-5
Sequence
Target Sequence 
>C. ELEGANS:175745_AT
caacaacccttttagcagtatgccggaatcagctgtgcactggattaaccgctttcaaac
gttccgccgaccaatcctatgcaccaacaactcgcgatctttttcacattcaccaccaac
aaaagcgaggcggaattgcgacagaatgttgtgagaagcgatgttcatttgcatatctca
aaacattctgctgcaatcaggacgataattgataacttattcatcatattttctcatcat
cgggtctcgtgcgctgcattattagactgcgcggaggcctctgatcaggtcaatatcgtt
ggcagctcaccggaccctaaccttttcttttctatctgaaaatatttgaattgttctctc
ctctctttgttttctcttctctgtcgttgccgtttccaatctttctctttcttgataata
atccaaatttttaattgcataatatgacaatacaaagtgataatagtgaaattgcctatc
cttcctctatttccctgtccaacagtttctggtctttattagtttgccacgatatatctt
tcaatccagtttgacctgatctcaacttcacagtcgtctgatgcatccattcttttta

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CAACAACCCTTTTAGCAGTATGCCG 75 191 177 Antisense
TGTGCACTGGATTAACCGCTTTCAA 21 555 210 Antisense
CAACAACTCGCGATCTTTTTCACAT 701 189 261 Antisense
ATCATATTTTCTCATCATCGGGTCT 79 31 399 Antisense
TCAATATCGTTGGCAGCTCACCGGA 650 631 466 Antisense
ATATTTGAATTGTTCTCTCCTCTCT 417 19 518 Antisense
TTTCCAATCTTTCTCTTTCTTGATA 544 669 569 Antisense
TAGTGAAATTGCCTATCCTTCCTCT 504 653 640 Antisense
ATTTCCCTGTCCAACAGTTTCTGGT 671 15 665 Antisense
GATCTCAACTTCACAGTCGTCTGAT 529 419 734 Antisense
TCGTCTGATGCATCCATTCTTTTTA 596 601 750 Antisense
Sequence Source GenBank