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[175721_s_at]

Description g1518843 /REP_DB=GenBank Identifier /GB=U63744.1 /PROTEIN_GB=AAC47308.1 /CHR=X /FEA=Mapped Transcript /DEF=p21-activated kinase CePAK
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 27      4grp_highest_8_8 > 41      4grp_high_8_8 > 49      6grp_highest_nonorm > 50      6grp_total > 07      4grp_total > 62      6grp_highest_sub > De      6grp_cgc > 11      6grp_high1_sub > Cu      6grp_cgc_sub > De      6grp_total_sub > Decrease      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 380
1 2 3 4 range
A 657.8
(3)
P 		482
(-0.4)
P 		513.5
(0.2)
P 		345.8
(-3)
P 		312
B 696.2
(3)
P 		512.4
(0.1)
P 		481.6
(-0.4)
P 		316.4
(-3)
P 		380
C 624.5
(3)
P 		462.8
(-0.7)
P 		459.9
(-0.7)
P 		359
(-3)
P 		266
avr 659.5
(3) 		485.7
(-0.3) 		485
(-0.3) 		340.4
(-3) 		319

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 384
1 2 3 4 5 6 range
A 653
(3)
P 		508
(-0.6)
P 		452
(-2)
P 		416
(-2.9)
P 		412.1
(-3)
P 		491.7
(-1)
P 		241
B 662
(3)
P 		450.4
(-0.7)
P 		452.1
(-0.7)
P 		535.5
(0.8)
P 		322.1
(-3)
P 		394.7
(-1.7)
P 		340
C 706
(3)
P 		461.2
(-1.1)
P 		502
(-0.5)
P 		379.7
(-2.5)
P 		351.3
(-3)
P 		444.3
(-1.4)
P 		355
avr 673.7
(3) 		473.2
(-0.9) 		468.7
(-0.9) 		443.7
(-1.4) 		361.8
(-3) 		443.6
(-1.4) 		312
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 189
1 2 3 4 range
A 239.5
(3)
P 		104.6
(-1.3)
P 		134.2
(-0.3)
P 		50.6
(-3)
P 		189
B 222
(3)
P 		126.5
(-0.7)
P 		111.6
(-1.3)
P 		67.4
(-3)
P 		155
C 206.7
(3)
P 		133
(-0)
P 		160.3
(1.1)
P 		60.5
(-3)
P 		146
avr 222.7
(3) 		121.4
(-0.7) 		135.4
(-0.2) 		59.5
(-3) 		163

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 185
1 2 3 4 5 6 range
A 120.4
(-1.4)
P 		180.7
(3)
P 		98.4
(-3)
P 		105.5
(-2.5)
P 		124.4
(-1.1)
P 		119.7
(-1.4)
P 		82
B 230.4
(3)
P 		149.9
(-1)
P 		109.8
(-3)
P 		141.8
(-1.4)
P 		130.8
(-2)
P 		147.2
(-1.1)
P 		121
C 261.7
(3)
P 		140.7
(-0.9)
P 		127.8
(-1.3)
P 		76.4
(-3)
P 		113.7
(-1.8)
P 		148.7
(-0.7)
P 		185
avr 204.2
(3) 		157.1
(0.1) 		112
(-2.7) 		107.9
(-3) 		123
(-2.1) 		138.5
(-1.1) 		96
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175721_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19956
Sequence Type Exemplar sequence
Representative Public ID 1518843 NCBI
Target Description g1518843 /REP_DB=GenBank Identifier /GB=U63744.1 /PROTEIN_GB=AAC47308.1 /CHR=X /FEA=Mapped Transcript /DEF=p21-activated kinase CePAK
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001029209(11), NM_001029207(11), NM_001029208(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001029209 NCBI Caenorhabditis elegans P21-Activated Kinase family member (pak-1) (pak-1) mRNA, complete cds. 11/11 None
NM_001029207 NCBI Caenorhabditis elegans P21-Activated Kinase family member (pak-1) (pak-1) mRNA, complete cds. 11/11 None
NM_001029208 NCBI Caenorhabditis elegans P21-Activated Kinase family member (pak-1) (pak-1) mRNA, complete cds. 11/11 None
C09B8.7e.3 ENSEMBL cdna:known chromosome:CEL140:X:6044266:6048568:-1 gene:C09B8.7 11/11 A 
C09B8.7e.2 ENSEMBL cdna:known chromosome:CEL140:X:6044267:6048568:-1 gene:C09B8.7 11/11 A 
C09B8.7e.1 ENSEMBL cdna:known chromosome:CEL140:X:6044275:6046119:-1 gene:C09B8.7 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
180873 4 3 NM_001029207 175721_s_at (11), 194208_x_at (11)
NM_001029208 175721_s_at (11), 194208_x_at (11)
NM_001029209 175721_s_at (11), 194208_x_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrX:6044265-6048562(-) 100.0 100.0
Public Domain and Genome References
Ensembl C09B8.7
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAL65775 P21-activated kinase family protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024378.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] dbj|BAA11844.1| protein kinase [Caenorhabditis elegans] 0.0
blast AAA68805 P21-activated kinase family protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024377.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] sp|Q17850|PAK1_CAEEL Serine/threonine-protein kinase pak-1 (p21-activated kinase 1) (PAK1) (CePAK) 0.0
blast AAC47308 p21-activated kinase CePAK [Caenorhabditis elegans] 0.0
blast AAO61438 P21-activated kinase family protein 1, isoform c [Caenorhabditis elegans] ref|NP_001024379.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] 0.0
blast AAA68805 P21-activated kinase family protein 1, isoform a [Caenorhabditis elegans] ref|NP_001024377.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] sp|Q17850|PAK1_CAEEL Serine/threonine-protein kinase pak-1 (p21-activated kinase 1) (PAK1) (CePAK) 7.0E-67
blast AAL65775 P21-activated kinase family protein 1, isoform b [Caenorhabditis elegans] ref|NP_001024378.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] dbj|BAA11844.1| protein kinase [Caenorhabditis elegans] 7.0E-67
blast AAO61440 P21-activated kinase family protein 1, isoform e [Caenorhabditis elegans] ref|NP_001024380.1| P21-Activated Kinase family member (pak-1) [Caenorhabditis elegans] 7.0E-67
Protein Families
Method ID Description E-Value
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 1.0E-62
ec A2S7_HUMAN (Q96Q40) Serine/threonine-protein kinase ALS2CR7 (EC 2.7.1.37) (Amyotrophic lateral sclerosis 2 chromosomal region candidate gene protein 7) 6.0E-63
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 7.0E-71
hanks 2.1.17 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; TWITCH 5.0E-71
hanks 1.1.1 AGC group; AGC I Cyclic nucleotide regulated protein kinase (PKA & PKG); cAPKa 4.0E-22
Protein Domains
Database ID Description E-Value
scop a.1.1.Globins All alpha proteins; Globin-like; Globin-like; Globins 8.0
scop a.1.1.Globins All alpha proteins; Globin-like; Globin-like; Globins 7.59999990463257
Pfam IPR000719 EMBL-EBI Protein kinase 3.8E-31
Pfam IPR000719 EMBL-EBI Protein kinase 5.0E-93
Pfam IPR000095 EMBL-EBI PAK-box/P21-Rho-binding 1.2E-29
Pfam IPR000719 EMBL-EBI Protein kinase 5.0E-93
Pfam IPR000095 EMBL-EBI PAK-box/P21-Rho-binding 1.2E-29
Sequence
Target Sequence 
>C. ELEGANS:175721_S_AT
atgttttctggaatcgatcacgagtaaaaattcaagccccttcattatttcacatcttcc
ccctctttatctcttctcgcaatgcacaatttggactatctcttttttcttgttgttgag
cgttcccaatcagacgacacacttcaattatttatgtaagttttttgtcttcactttgtc
ttcttttcattcggtttcttgcatttgtgtcgttcgaacggaaatctacagttaaccagc
tttgcccaaatttccctatttctttttcaagtgtctgtaggtgggaactattgaaactct
gttttcatgtggtttccaa

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
ATGTTTTCTGGAATCGATCACGAGT 681 47 1914 Antisense
AATTCAAGCCCCTTCATTATTTCAC 109 177 1942 Antisense
TTTATCTCTTCTCGCAATGCACAAT 333 673 1979 Antisense
ATTTGGACTATCTCTTTTTTCTTGT 124 19 2002 Antisense
TTTTTCTTGTTGTTGAGCGTTCCCA 540 675 2017 Antisense
TTCCCAATCAGACGACACACTTCAA 430 695 2036 Antisense
TAAGTTTTTTGTCTTCACTTTGTCT 624 631 2070 Antisense
TCTTCTTTTCATTCGGTTTCTTGCA 370 617 2092 Antisense
TCTTGCATTTGTGTCGTTCGAACGG 492 617 2110 Antisense
TCTACAGTTAACCAGCTTTGCCCAA 707 611 2138 Antisense
AACTCTGTTTTCATGTGGTTTCCAA 526 139 2208 Antisense
Sequence Source GenBank