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[175713_at]

Description g10242309 /REP_DB=GenBank Identifier /GB=AF285179.1 /PROTEIN_GB=AAG15377.1 /CHR=2 /FEA=Mapped Transcript /DEF=zygote defective-1
Cluster 6grp_highest_10_10 > 96      6grp_high1_10_10 > 79      4grp_highest_8_8 > 43      4grp_high_8_8 > 24      6grp_highest_nonorm > 91      6grp_total > 79      4grp_total > 55      6grp_highest_sub > Cu      6grp_cgc > 59      6grp_high1_sub > Cu      6grp_cgc_sub > Da      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 147
1 2 3 4 range
A 251.4
(3)
P 		140.8
(-3)
P 		147.9
(-2.6)
P 		158
(-2.1)
P 		111
B 231.4
(3)
P 		136.5
(-3)
P 		165.1
(-1.2)
P 		200
(1)
P 		95
C 267.6
(3)
P 		121.1
(-3)
P 		242.8
(2)
P 		169.1
(-1)
P 		147
avr 250.1
(3) 		132.8
(-3) 		185.3
(-0.3) 		175.7
(-0.8) 		117

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 278
1 2 3 4 5 6 range
A 138.3
(-2.5)
P 		173.3
(-1.6)
P 		116.9
(-3)
P 		124.7
(-2.8)
P 		306.1
(1.8)
P 		354.8
(3)
P 		238
B 206.7
(-1)
P 		242.8
(-0.1)
P 		128.8
(-3)
P 		210.3
(-0.9)
P 		277
(0.8)
P 		363.9
(3)
P 		235
C 163.1
(-0.8)
P 		170.8
(-0.6)
P 		146.7
(-1.3)
P 		85.6
(-3)
P 		210.1
(0.5)
P 		296.9
(3)
P 		211
avr 169.4
(-1.9) 		195.6
(-1.1) 		130.8
(-3) 		140.2
(-2.7) 		264.4
(0.9) 		338.5
(3) 		208
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 68
1 2 3 4 range
A 91.5
(3)
P 		30.6
(-2.3)
P 		38.7
(-1.6)
P 		23.1
(-3)
P 		68
B 73.8
(3)
P 		33.7
(-3)
P 		38.3
(-2.3)
P 		42.6
(-1.7)
P 		40
C 88.6
(3)
P 		34.8
(-2.4)
P 		84.6
(2.6)
P 		28.5
(-3)
P 		60
avr 84.6
(3) 		33
(-2.8) 		53.9
(-0.5) 		31.4
(-3) 		53

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 119
1 2 3 4 5 6 range
A 25.5
(-3)
P 		61.6
(0.2)
P 		25.5
(-3)
P 		31.6
(-2.5)
P 		92.4
(3)
P 		86.4
(2.5)
P 		67
B 71.9
(-0.7)
P 		80.8
(-0.2)
P 		31.3
(-3)
P 		55.7
(-1.6)
P 		112.5
(1.7)
P 		135.7
(3)
P 		104
C 60.4
(0.2)
P 		52.1
(-0.5)
P 		37.3
(-1.5)
P 		17.2
(-3)
P 		68
(0.7)
P 		99.4
(3)
P 		82
avr 52.6
(-1.3) 		64.8
(-0.4) 		31.4
(-3) 		34.8
(-2.7) 		91
(1.7) 		107.2
(3) 		76
NetAffx Links Cluster Members
Consensus/Exemplar
GeneChip Array Information
Probe Set ID 175713_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19746
Sequence Type Exemplar sequence
Representative Public ID 10242309 NCBI
Target Description g10242309 /REP_DB=GenBank Identifier /GB=AF285179.1 /PROTEIN_GB=AAG15377.1 /CHR=2 /FEA=Mapped Transcript /DEF=zygote defective-1
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_062702(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_062702 NCBI Caenorhabditis elegans ZYGote defective: embryonic lethal family member (zyg-1) (zyg-1) mRNA, complete cds. 11/11 A 
F59E12.2.1 ENSEMBL cdna:known chromosome:CEL140:II:5649706:5654334:1 gene:F59E12.2 10/11 None
F59E12.2.2 ENSEMBL cdna:known chromosome:CEL140:II:5649717:5654391:1 gene:F59E12.2 11/11 None
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrII:5649712-5654389(+) 97.82 100.0
Public Domain and Genome References
Gene Title
Gene Symbol zyg-1
Ensembl F59E12.2
Entrez Gene 173956 Entrez gene
SwissProt Q9GT24 EMBL-EBI
WormBase CE28571 Wormbase
Functional Annotations
Gene Ontology
GO Biological Process (view graph)
ID Description Evidence Links
6468 protein amino acid phosphorylation inferred from electronic annotation QuickGO AmiGO
GO Cellular Component (view graph)
ID Description Evidence Links
5813 centrosome inferred from direct assay QuickGO AmiGO
GO Molecular Function (view graph)
ID Description Evidence Links
4672 protein kinase activity inferred from electronic annotation QuickGO AmiGO
4674 protein serine/threonine kinase activity inferred from electronic annotation QuickGO AmiGO
4713 protein-tyrosine kinase activity inferred from electronic annotation QuickGO AmiGO
5524 ATP binding inferred from electronic annotation QuickGO AmiGO
4672 protein kinase activity inferred from direct assay QuickGO AmiGO
Protein Similarities
Method ID Description E-Value
blast AAB54253 Zygote defective: embryonic lethal protein 1 [Caenorhabditis elegans] ref|NP_495103.1| ZYGote defective: embryonic lethal family member (zyg-1) [Caenorhabditis elegans] gb|AAG15377.1| zygote defective-1 [Caenorhabditis elegans] sp|Q9GT24|ZYG1_CAEEL Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) 0.0
blast Q621J7 Probable serine/threonine-protein kinase zyg-1 (Zygote defective protein 1) emb|CAE59156.1| Hypothetical protein CBG02462 [Caenorhabditis briggsae] 0.0
blast T15269 hypothetical protein F59E12.1 - Caenorhabditis elegans 8.0E-95
Protein Families
Method ID Description E-Value
hanks 2.1.25 CaMK Group; CaMK I Regulated by Ca2+/CaM and close relatives; CDPK 9.0E-61
Protein Domains
Database ID Description E-Value
Pfam IPR000719 EMBL-EBI Protein kinase 5.9E-65
Sequence
Target Sequence 
>C. ELEGANS:175713_AT
cttagtatacgatgagttttcctcttacttcatgtgctcctacattttaccacatatctt
tctgttaatcctacacattcaagtttcaaatgatcttcagtcattctaatgattctcttt
atttttcttcttc

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
CTTAGTATACGATGAGTTTTCCTCT 225 221 2171 Antisense
GATGAGTTTTCCTCTTACTTCATGT 53 409 2181 Antisense
TCTTACTTCATGTGCTCCTACATTT 80 617 2193 Antisense
GTGCTCCTACATTTTACCACATATC 469 479 2204 Antisense
CCTACATTTTACCACATATCTTTCT 201 269 2209 Antisense
TACCACATATCTTTCTGTTAATCCT 101 651 2218 Antisense
ATCTTTCTGTTAATCCTACACATTC 627 31 2226 Antisense
GTTAATCCTACACATTCAAGTTTCA 609 399 2234 Antisense
TCAAGTTTCAAATGATCTTCAGTCA 398 631 2249 Antisense
GATCTTCAGTCATTCTAATGATTCT 505 419 2262 Antisense
ATGATTCTCTTTATTTTTCTTCTTC 313 47 2279 Antisense
Sequence Source GenBank