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[175706_s_at]

Description g5734145 /REP_DB=GenBank Identifier /GB=AF166169.1 /PROTEIN_GB=AAD49858.1 /CHR=5 /FEA=Mapped Transcript /DEF=beta-G spectrin
Cluster 6grp_highest_10_10 > 60      6grp_high1_10_10 > 17      4grp_highest_8_8 > 48      4grp_high_8_8 > 42      6grp_highest_nonorm > 20      6grp_total > 16      4grp_total > 53      6grp_highest_sub > De      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 1766
1 2 3 4 range
A 2216.5
(3)
P 		1427.6
(0.3)
P 		1163.6
(-0.6)
P 		477.9
(-3)
P 		1739
B 2114.3
(3)
P 		1364.2
(0.3)
P 		1058.8
(-0.8)
P 		466.4
(-3)
P 		1648
C 1985.2
(3)
P 		1558
(1.3)
P 		1145.9
(-0.3)
P 		450.2
(-3)
P 		1535
avr 2105.3
(3) 		1449.9
(0.6) 		1122.8
(-0.6) 		464.8
(-3) 		1641

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 1710
1 2 3 4 5 6 range
A 2333.7
(3)
P 		1432.5
(-0.3)
P 		821.4
(-2.6)
P 		710.7
(-3)
P 		1152.5
(-1.4)
P 		729.3
(-2.9)
P 		1623
B 2023.6
(3)
P 		1518.4
(0.8)
P 		654.6
(-2.9)
P 		991.6
(-1.4)
P 		736.7
(-2.5)
P 		626.6
(-3)
P 		1397
C 2240.9
(3)
P 		1496.1
(0.2)
P 		935.5
(-1.8)
P 		623.9
(-3)
P 		718.4
(-2.6)
P 		638.2
(-2.9)
P 		1617
avr 2199.4
(3) 		1482.3
(0.2) 		803.8
(-2.5) 		775.4
(-2.6) 		869.2
(-2.2) 		664.7
(-3) 		1535
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 737
1 2 3 4 range
A 807
(3)
P 		309.9
(-1)
P 		304.2
(-1.1)
P 		69.9
(-3)
P 		737
B 674.2
(3)
P 		336.9
(-0.5)
P 		245.3
(-1.5)
P 		99.4
(-3)
P 		575
C 657.2
(3)
P 		447.9
(0.8)
P 		399.4
(0.3)
P 		75.9
(-3)
P 		581
avr 712.8
(3) 		364.9
(-0.3) 		316.3
(-0.8) 		81.7
(-3) 		631

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 705
1 2 3 4 5 6 range
A 430.2
(1.6)
P 		509.5
(3)
P 		178.9
(-3)
P 		180.2
(-3)
P 		347.8
(0.1)
P 		177.6
(-3)
P 		332
B 704.1
(3)
P 		505.4
(0.8)
P 		159
(-3)
P 		262.6
(-1.9)
P 		299.3
(-1.5)
P 		233.7
(-2.2)
P 		545
C 830.6
(3)
P 		456.5
(-0.2)
P 		238.1
(-2)
P 		125.5
(-3)
P 		232.4
(-2.1)
P 		213.7
(-2.2)
P 		705
avr 655
(3) 		490.5
(0.9) 		192
(-3) 		189.4
(-3) 		293.2
(-1.7) 		208.3
(-2.8) 		466
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175706_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19900
Sequence Type Exemplar sequence
Representative Public ID 5734145 NCBI
Target Description g5734145 /REP_DB=GenBank Identifier /GB=AF166169.1 /PROTEIN_GB=AAD49858.1 /CHR=5 /FEA=Mapped Transcript /DEF=beta-G spectrin
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028881(11), NM_001028882(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028881 NCBI Caenorhabditis elegans UNCoordinated family member (unc-70) (unc-70) mRNA, complete cds. 11/11 None
NM_001028882 NCBI Caenorhabditis elegans UNCoordinated family member (unc-70) (unc-70) mRNA, complete cds. 11/11 None
K11C4.3a ENSEMBL cdna:known chromosome:CEL140:V:6883937:6894814:1 gene:K11C4.3 11/11 A 
K11C4.3b ENSEMBL cdna:known chromosome:CEL140:V:6883983:6893996:1 gene:K11C4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6884182-6893997(+) 100.0 100.0
Public Domain and Genome References
Ensembl K11C4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK77611 Uncoordinated protein 70, isoform a [Caenorhabditis elegans] ref|NP_001024052.1| UNCoordinated family member (unc-70) [Caenorhabditis elegans] gb|AAF72996.1| beta-spectrin [Caenorhabditis elegans] gb|AAD49859.1| beta-G spectrin [Caenorhabditis elegans] 0.0
blast AAK77612 Uncoordinated protein 70, isoform b [Caenorhabditis elegans] ref|NP_001024053.1| UNCoordinated family member (unc-70) [Caenorhabditis elegans] 0.0
blast AAD49858 beta-G spectrin [Caenorhabditis elegans] 0.0
blast CAE71967 Hypothetical protein CBG19038 [Caenorhabditis briggsae] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 6.0
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 5.90000009536743
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.4E-29
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-38
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.3E-17
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.9E-11
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.0E-26
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.2E-15
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.2E-14
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.4E-17
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.0E-29
Pfam IPR002017 EMBL-EBI Spectrin repeat 3.3E-18
Pfam IPR002017 EMBL-EBI Spectrin repeat 4.7E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.8E-19
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.6E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.7E-13
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.8E-26
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.3E-25
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.8E-25
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.9E-19
Pfam IPR002017 EMBL-EBI Spectrin repeat 3.6E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 5.5E-13
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.3E-17
Sequence
Target Sequence 
>C. ELEGANS:175706_S_AT
taatcccagtcagccaatgagctccccaattcatacggtttcattcttttcaccacccat
tcttcgttttgttttgtttccccccttttttctctgtcctaaacgtcccccctattccat
attaggcattaatattatattcgtttaaatttcccccctacactttctcatactttgaaa
gctttttgcttattagccttcatatttttttgtgcagattacaagtagtagaaccaaaga
tagccttgcccgttcgtcatcggtctctttttgattgggaaatcggcgtctcaattatta
ttgatttttcttaaatatacaattagtatttgatattaaaaatcaaagaaaaaaaaagag
aaatgtgatgtacgggcaccaattcgaactcggtttgttgtatccccttaatttttaatg
tagctatctttcgttattttttgtctctttcagcttcacactgcttttcaattcaagaaa
tctctttttttcccaccataccaattctattggttccaattttgcaccaaaacccttgct
ttcattttttagactccaagaaacttccactttt

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATCCCAGTCAGCCAATGAGCTCC 442 635 7180 Antisense
CATACGGTTTCATTCTTTTCACCAC 466 213 7211 Antisense
TCCCCCCTATTCCATATTAGGCATT 656 595 7285 Antisense
AGAACCAAAGATAGCCTTGCCCGTT 539 73 7409 Antisense
TCGTCATCGGTCTCTTTTTGATTGG 533 603 7433 Antisense
GATGTACGGGCACCAATTCGAACTC 337 399 7546 Antisense
ATGTAGCTATCTTTCGTTATTTTTT 668 47 7597 Antisense
CTTTCAGCTTCACACTGCTTTTCAA 42 223 7626 Antisense
TTTCCCACCATACCAATTCTATTGG 175 671 7668 Antisense
TTTGCACCAAAACCCTTGCTTTCAT 491 667 7700 Antisense
TAGACTCCAAGAAACTTCCACTTTT 344 653 7729 Antisense
Sequence Source GenBank