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[175686_s_at]

Description g8118452 /REP_DB=GenBank Identifier /GB=AF261891.1 /PROTEIN_GB=AAF72996.1 /CHR=5 /FEA=Mapped Transcript /DEF=beta-spectrin
Cluster 6grp_high1_10_10 > 16      4grp_high_8_8 > 34      6grp_total > 17      4grp_total > 45      6grp_cgc > 13      6grp_high1_sub > De      6grp_cgc_sub > De      6grp_total_sub > Decrease      6grp_rule_based > S1      4grp_rule_based > L1      

Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 387
1 2 3 4 range
A 566.9
(3)
P 		402.5
(-0.1)
P 		286.2
(-2.2)
P 		245
(-3)
P 		322
B 632.3
(3)
P 		451.7
(-0.2)
P 		291.1
(-3)
P 		306.5
(-2.7)
P 		341
C 435.1
(2.4)
P 		456.2
(3)
P 		371
(0.5)
A 		251.3
(-3)
P 		205
avr 544.8
(3) 		436.8
(0.7) 		316.1
(-2) 		267.6
(-3) 		277

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 511
1 2 3 4 5 6 range
A 615
(3)
P 		471
(0.1)
P 		368.4
(-1.9)
P 		314.8
(-3)
P 		407.9
(-1.1)
P 		358.9
(-2.1)
P 		300
B 526
(3)
P 		441.1
(1.1)
P 		271.4
(-2.7)
P 		373.8
(-0.4)
P 		256.1
(-3)
P 		281.3
(-2.4)
P 		270
C 699.8
(3)
P 		519.6
(0.9)
P 		345.2
(-1.2)
P 		188.8
(-3)
A 		301.6
(-1.7)
P 		362.9
(-1)
P 		511
avr 613.6
(3) 		477.2
(0.5) 		328.3
(-2.3) 		292.5
(-3) 		321.9
(-2.5) 		334.4
(-2.2) 		321
No Scale
G range Absolute graph Relative graph Expression Table Dauer Comp.
4 171
1 2 3 4 range
A 206.4
(3)
P 		87.4
(-1.2)
P 		74.8
(-1.6)
P 		35.9
(-3)
P 		171
B 201.6
(3)
P 		111.6
(-1)
P 		67.4
(-2.9)
P 		65.3
(-3)
P 		136
C 144
(3)
P 		131.2
(2.2)
P 		129.3
(2.1)
A 		42.4
(-3)
P 		102
avr 184
(3) 		110.1
(-0.3) 		90.5
(-1.1) 		47.9
(-3) 		136

1 : 1 state (S1, L1)
2 : dauer (ST, LT)
6 221
1 2 3 4 5 6 range
A 113.4
(-0.7)
P 		167.5
(3)
P 		80.2
(-3)
P 		79.8
(-3)
P 		123.1
(-0)
P 		87.4
(-2.5)
P 		88
B 183
(3)
P 		146.8
(1.1)
P 		65.9
(-3)
P 		99
(-1.3)
P 		104.1
(-1)
P 		104.9
(-1)
P 		117
C 259.4
(3)
P 		158.6
(0.3)
P 		87.9
(-1.6)
P 		38
(-3)
A 		97.6
(-1.4)
P 		121.5
(-0.7)
P 		221
avr 185.3
(3) 		157.6
(1.5) 		78
(-2.7) 		72.3
(-3) 		108.3
(-1.1) 		104.6
(-1.3) 		113
NetAffx Links Cluster Members
Consensus/Exemplar
Group Members
GeneChip Array Information
Probe Set ID 175686_s_at
GeneChip Array C. elegans Genome Array
Organism Common Name Nematode
Probe Design Information
Transcript ID(Array Design) affy.Ce.19905
Sequence Type Exemplar sequence
Representative Public ID 8118452 NCBI
Target Description g8118452 /REP_DB=GenBank Identifier /GB=AF261891.1 /PROTEIN_GB=AAF72996.1 /CHR=5 /FEA=Mapped Transcript /DEF=beta-spectrin
Cluster Evidence This cluster is supported by a Mapped Transcript.
Annotation Method Description
Annotation Description This probe set was annotated using the Matching Probes based pipeline to a RefSeq identifier using 1 transcript(s).
Annotation Grade This is a grade A annotation.
Annotation Transcript Cluster (# of Matching Probes) NM_001028881(11)
Transcript Assignments
Representative
Transcript		 Description Matching Probes Related Probesets by Grade
NM_001028881 NCBI Caenorhabditis elegans UNCoordinated family member (unc-70) (unc-70) mRNA, complete cds. 11/11 None
K11C4.3a ENSEMBL cdna:known chromosome:CEL140:V:6883937:6894814:1 gene:K11C4.3 11/11 A 
Annotation Notes
There are no noteworthy cross hybridizing mRNAs found for this probe set.
Splice Variants
Entrez Total Refseq Isoforms Total Detected By This Probeset Refseq Probesets
179077 2 1 NM_001028881 175686_s_at (11)
Genomic Alignment of Consensus/Exemplar Sequence
Assembly Wormbase March 2004
Alignment(s)
Position Identity Coverage Cytoband
chrV:6883935-6894818(+) 100.0 100.0
Public Domain and Genome References
Ensembl K11C4.3
Functional Annotations
Protein Similarities
Method ID Description E-Value
blast AAK77611 Uncoordinated protein 70, isoform a [Caenorhabditis elegans] ref|NP_001024052.1| UNCoordinated family member (unc-70) [Caenorhabditis elegans] gb|AAF72996.1| beta-spectrin [Caenorhabditis elegans] gb|AAD49859.1| beta-G spectrin [Caenorhabditis elegans] 0.0
blast AAK77612 Uncoordinated protein 70, isoform b [Caenorhabditis elegans] ref|NP_001024053.1| UNCoordinated family member (unc-70) [Caenorhabditis elegans] 0.0
blast AAD49858 beta-G spectrin [Caenorhabditis elegans] 0.0
Protein Domains
Database ID Description E-Value
scop a.2.3.Chaperone J-domain All alpha proteins; Long alpha-hairpin; Chaperone J-domain; Chaperone J-domain 5.90000009536743
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 2.4E-29
Pfam IPR001715 EMBL-EBI Calponin-like actin-binding 1.6E-38
Pfam IPR001849 EMBL-EBI Pleckstrin-like 1.3E-17
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.9E-11
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.0E-26
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.2E-15
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.2E-14
Pfam IPR002017 EMBL-EBI Spectrin repeat 6.4E-17
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.0E-29
Pfam IPR002017 EMBL-EBI Spectrin repeat 3.3E-18
Pfam IPR002017 EMBL-EBI Spectrin repeat 4.7E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.8E-19
Pfam IPR002017 EMBL-EBI Spectrin repeat 9.6E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.7E-13
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.8E-26
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.3E-25
Pfam IPR002017 EMBL-EBI Spectrin repeat 1.8E-25
Pfam IPR002017 EMBL-EBI Spectrin repeat 2.9E-19
Pfam IPR002017 EMBL-EBI Spectrin repeat 3.6E-24
Pfam IPR002017 EMBL-EBI Spectrin repeat 5.5E-13
Sequence
Target Sequence 
>C. ELEGANS:175686_S_AT
taatgtccctttacctacagtaatccccaccagaggaagtagtgagacccagaattttta
tgagaaaattcgcttgtccttccttcgattgccagcttagtctttatttgtgagaaatta
tttggatttctcttggattttatttctgtggcagctttatttctttttttgtttttcacc
tttggttcactttggaactaatgaatcttttgactaaaccatggttttttctaacattct
aacaacactcttggggccatttttggacgacgattatgtattggtttgaaatggaaaaaa
accaaaaagttggagagagagaacgaaaggaagtcgtcgttttgccaccagtttgctttg
ctttctgcaaaattgccgtcgaaatggactcgaacctcgtcaaaaagcattgtgcgttaa
gctttgaagccgtacatagaaaattaaatgttttgtggttattttctgtatagagaggac
agatgttaagctcgccgagct

BLASTn GenBank NR
Probe Info
Probe Sequence(5'-3') Probe X Probe Y Probe Interrogation
Position 		Strandedness
TAATGTCCCTTTACCTACAGTAATC 547 635 7925 Antisense
CCTACAGTAATCCCCACCAGAGGAA 252 269 7938 Antisense
TGAGAAAATTCGCTTGTCCTTCCTT 414 567 7985 Antisense
CCTTCGATTGCCAGCTTAGTCTTTA 664 267 8006 Antisense
TTCTGTGGCAGCTTTATTTCTTTTT 364 693 8068 Antisense
TGTTTTTCACCTTTGGTTCACTTTG 597 561 8094 Antisense
ACATTCTAACAACACTCTTGGGGCC 266 105 8158 Antisense
TCTTGGGGCCATTTTTGGACGACGA 628 617 8173 Antisense
CACCAGTTTGCTTTGCTTTCTGCAA 29 203 8270 Antisense
GAAATGGACTCGAACCTCGTCAAAA 451 361 8305 Antisense
GGACAGATGTTAAGCTCGCCGAGCT 120 525 8401 Antisense
Sequence Source GenBank